本文選題：光波 + 消毒滅菌��； 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2014年01期

【摘要】：目的探討光波對(duì)口腔實(shí)心金屬器械消毒滅菌的溫效、時(shí)效關(guān)系。方法采用人工污染實(shí)驗(yàn)法,以無(wú)菌口鏡、鑷子和探針為載體,用不同指示菌和HBsAg純抗原進(jìn)行污染,經(jīng)不同溫度、對(duì)應(yīng)不同時(shí)間的光波消毒滅菌后進(jìn)行采樣,最后采用增菌培養(yǎng)和酶聯(lián)免疫吸附法、雅培檢測(cè)法來(lái)分別檢測(cè)光波對(duì)細(xì)菌的滅活情況和對(duì)HBsAg抗原的破壞情況。結(jié)果光波在120℃時(shí)5 min,130℃時(shí)3 min,140℃時(shí)1 min都能徹底殺滅金黃色葡萄球菌和綠膿桿菌;在120℃時(shí)7 min,130℃時(shí)5 min,140℃時(shí)3 min都能徹底殺滅白色念珠菌;在120℃時(shí)9 min,130℃時(shí)7 min,140℃時(shí)5 min都能徹底殺滅枯草芽孢桿菌黑色變種。光波在120℃時(shí)7 min,130℃時(shí)5 min,140℃時(shí)3 min,都能有效破壞HBsAg的抗原性。經(jīng)抗力實(shí)驗(yàn)比較,口腔自然菌比枯草芽孢桿菌黑色變種更易滅活。結(jié)論光波能夠?qū)崿F(xiàn)對(duì)口腔實(shí)心金屬器械的快速消毒滅菌,并且在器械能耐高溫的前提下,溫度越高則時(shí)間越短、效果越好。
[Abstract]:Objective to investigate the effect of light wave on sterilizing and sterilizing oral solid metal instruments.Methods the method of artificial contamination experiment was used, using sterilizing microscope, tweezers and probe as the carrier, using different indicator bacteria and pure antigen of HBsAg, sterilizing and sampling with different temperature and corresponding light wave at different time.In the end, bacteria culture, enzyme linked immunosorbent assay (Elisa) and Abbott assay were used to detect the inactivation of bacteria by light wave and the destruction of HBsAg antigen respectively.Results the light wave could kill Staphylococcus aureus and Pseudomonas aeruginosa completely at 1 min at 5 mins 130 鈩，

本文編號(hào)：1754860