目的檢測與Toll樣受體2（Toll-like receptor 2,TLR2）呈負(fù)相關(guān)性的微小RNA（microRNA,miRNA）在人牙周膜成纖維細胞（human periodontal ligament fibroblasts,hPDLFs）中與TLR2的作用關(guān)系及其對骨唾液酸蛋白（bone sialoprotein,BSP）的調(diào)控作用。方法查找與TLR2具有靶向結(jié)合關(guān)系且呈負(fù)相關(guān)性的miRNA（miR-23a、miR-144、miR-19a）, Real-time PCR方法檢測正常及牙齦卟啉單胞菌（Porphyromonas gingivals,P.gingivalis）胞壁成分脂多糖（lipopolysaccharide,LPS）誘導(dǎo)下hPDLFs中目標(biāo)miRNA的mRNA表達水平;分空白對照組、陰性對照（LPS）組、miRNA過表達對照組、miRNA過表達組、miRNA抑制對照組、miRNA抑制組,用Lipofectamine 2000分別轉(zhuǎn)染hPDLFs,Real-time PCR及Western Blot法分別測定TLR2、BSP的mRNA及蛋白表達水平。結(jié)果經(jīng)LPS... 

【文章來源】：口腔醫(yī)學(xué). 2020,40(07)

【文章頁數(shù)】：6 頁

【部分圖文】：

第4代hPDLFs(倒置顯微鏡 ×40)

LPS誘導(dǎo)hPDLFs后TLR2的表達

正常及LPS誘導(dǎo)下hPDLFs中miRNA的表達

【參考文獻】：
期刊論文
[1]MicroRNAs and immunity in periodontal health and disease[J]. Xianghong Luan,Xiaofeng Zhou,Afsar Naqvi,Marybeth Francis,Deborah Foyle,Salvador Nares,Thomas G.H.Diekwisch.  International Journal of Oral Science. 2018(03)
[2]微小RNAs在牙周致病過程中的調(diào)控機制研究進展[J]. 姜慕舟,黃婉怡,林莉.  中國實用口腔科雜志. 2017(01)
[3]Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues[J]. Yu-feng Xie1, Rong Shu1*, Shao-yun Jiang1, Da-li Liu1, Xiu-li Zhang2 1Department of Periodontology, Shanghai Ninth People’s Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 2Shanghai Research Institute of Stomatology, Shanghai Ninth People’s Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.  International Journal of Oral Science. 2011(03)



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