發(fā)布時間：2019-07-13 16:59

【摘要】：目的:探討功能性下頜偏斜對青春期大鼠下頜髁突軟骨增殖活動的影響。方法:選用4周齡雄性SD大鼠80只,使用隨機數(shù)字表法隨機分成3組:偏斜組40只,偏斜恢復組20只,對照組20只。偏斜組及偏斜恢復組大鼠上前牙黏結(jié)冠套模擬功能性下頜偏斜。偏斜恢復組在安放冠套14 d后全部拆除,對照組不作任何處理。偏斜組及對照組分別在7、14、21 d及28 d各取10只與5只大鼠處死,偏斜恢復組在拆除冠套后的第7、14天各取10只大鼠處死。免疫組織化學SABC法檢測下頜髁突軟骨細胞增殖細胞核抗原(proliferating cell nuclear antigen,PCNA)的表達。結(jié)果:(1)PCNA陽性細胞主要分布于髁突軟骨增殖層和淺肥大層。(2)實驗第7天時,偏斜組非偏斜側(cè)髁突軟骨中、后、外區(qū)域PCNA表達較對照組增強(中部P=0.022,后部P=0.019,外部P=0.024),偏斜側(cè)髁突軟骨后、外區(qū)域PCNA表達較對照組減弱(后部P=0.015,外部P=0.017),實驗第14天差異更加顯著(非偏斜側(cè)中部P=0.0023,后部P=0.01,外部P=0.0097,偏斜側(cè)后部P=0.0005,外部P=0.006),且差異性一直持續(xù)到第28天(非偏斜側(cè)中部P=0.0001,后部P=0.0001,外部P=0.0001,偏斜側(cè)后部P=0.0001,外部P=0.0001)。當拆除冠套后,偏斜恢復組兩側(cè)髁突軟骨PCNA表達的差異逐漸減小,至實驗28 d時恢復至對照組水平。結(jié)論:功能性下頜偏斜使青春期大鼠下頜骨雙側(cè)髁突軟骨發(fā)生不對稱適應性改建,而進行早期矯治后,雙側(cè)髁突軟骨的生長改建活動逐漸趨于一致。
[Abstract]:Objective: to investigate the effect of functional mandibular deviation on the proliferation of mandibular condylar cartilage in adolescent rats. Methods: 80 4-week-old male SD rats were randomly divided into three groups: oblique group (n = 40), oblique recovery group (n = 20) and control group (n = 20). Functional mandibular deviation was simulated by bonding crown cover of upper anterior teeth in oblique group and oblique recovery group. The oblique recovery group was removed 14 days after placing the crown sleeve, while the control group was not treated with any treatment. 10 rats and 5 rats in the oblique group and the control group were killed on the 7th, 14th, 21st and 28th day, respectively, and 10 rats in the oblique recovery group were killed on the 7th and 14th day after the crown cover was removed. The expression of proliferating cell nuclear antigen (proliferating cell nuclear antigen,PCNA) in mandibular condylar cartilage cells was detected by SABC. Results: (1) PCNA positive cells were mainly distributed in the proliferating layer and superficial hypertrophic layer of condylar cartilage. (2) on the 7th day of the experiment, the expression of PCNA in the non-oblique condylar cartilage of the oblique group was higher than that of the control group (P 鈮，

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