【摘要】：目的比較復(fù)合羥固醇無(wú)機(jī)小牛骨塊(羥固醇骨塊)和無(wú)機(jī)小牛骨粉在比格犬下頜骨臨界骨缺損動(dòng)物模型早期愈合階段的成骨效應(yīng)。方法選取健康雄性比格犬4只,拔除雙側(cè)下頜第3和第4前磨牙以及第1磨牙(P3-M1)后,雙側(cè)下頜骨各復(fù)制2個(gè)5 mm×10 mm×10 mm標(biāo)準(zhǔn)化箱型臨界骨缺損。4周愈合期后,按半口對(duì)照設(shè)計(jì),隨機(jī)將羥固醇骨塊和無(wú)機(jī)小牛骨粉植入骨缺損。術(shù)后4周處死,切取標(biāo)本制作石蠟和硬組織切片,行組織學(xué)觀察及組織形態(tài)學(xué)測(cè)量。結(jié)果實(shí)驗(yàn)動(dòng)物愈合良好,羥固醇骨塊組及無(wú)機(jī)小牛骨粉組均見臨界骨缺損邊緣及骨材料-宿主骨交界處明顯的成骨細(xì)胞及新骨形成。HE染色組織形態(tài)學(xué)測(cè)量結(jié)果顯示羥固醇骨塊組和無(wú)機(jī)小牛骨粉組新生骨面積比分別為(37.22±2.16)%和(34.70±2.66)%,兩者比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);羥固醇骨塊組成骨細(xì)胞數(shù)[(31.13±2.85)]與無(wú)機(jī)小牛骨粉組[(24.88±2.95)]比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。硬組織切片亞甲基藍(lán)-酸性品紅染色組織形態(tài)學(xué)測(cè)量結(jié)果顯示羥固醇骨塊及無(wú)機(jī)小牛骨粉骨材料新生骨面積相對(duì)百分比分別為(20.13±1.32)%和(19.28±0.82)%,骨材料相對(duì)存留率分別為(30.80±1.16)%和(29.69±1.12)%,兩者比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論羥固醇骨塊在犬下頜骨臨界骨缺損早期骨愈合階段具有類似于小牛骨粉的成骨效果。由于羥固醇骨塊具有良好的空間維持能力,更適合于臨床大面積骨缺損的修復(fù)。
[Abstract]:Objective to compare the osteogenic effects of compound hydroxysterol inorganic calf bone mass (hydroxysterol bone mass) and inorganic calf bone powder in the early healing stage of mandibular critical bone defect in Beagle dogs. Methods four healthy male Beagle dogs were selected. After bilateral mandibular third and fourth premolars and first molar (P3-M1) were removed, two 5 mm 脳 10 mm standardized box critical bone defects were replicated in each side of the jaw. After 4 weeks of healing, the hydroxysterol bone mass and inorganic calf bone powder were randomly implanted into bone defects according to half mouth control design. Four weeks after operation, the specimens were cut to make paraffin and hard tissue sections, and the histology and histomorphology were observed. Results the experimental animals healed well. There were obvious osteoblasts and new bone formation at the edge of critical bone defect and the junction of bone material and host bone in the hydroxysterol bone mass group and the inorganic calf bone powder group. The histomorphology of HE staining showed that the ratio of new bone area in the hydroxysterol bone mass group and the inorganic calf bone powder group was (37.22 鹵2.16)% and (34.70 鹵2.66)%, respectively, and there was no significant difference between the two groups (P 0.05). The number of bone cells composed of hydroxysterol bone mass [(31.13 鹵2.85)] was significantly different from that of inorganic calf bone powder group [(24.88 鹵2.95)] (P 0.05). The relative percentage of new bone area of hydroxysterol bone mass and inorganic calf bone powder was (20.13 鹵1.32)% and (19.28 鹵0.82)%, respectively, and the relative retention rate of bone material was (30.80 鹵1.16)% and (29.69 鹵1.12)%, respectively. there was no significant difference between the two groups (P 0.05). The results showed that the relative percentage of new bone area was (20.13 鹵1.32)% and (19.28 鹵0.82)%, and the relative retention rate of bone material was (30.80 鹵1.16)% and (29.69 鹵1.12)%, respectively. Conclusion the osteogenic effect of hydroxysterol bone mass is similar to that of calf bone powder in the early stage of bone healing of mandibular critical bone defect in dogs. Because the hydroxysterol bone mass has good spatial maintenance ability, it is more suitable for the repair of large area bone defect in clinic.
【作者單位】： 南方醫(yī)科大學(xué)口腔醫(yī)院(廣東省口腔醫(yī)院);
【基金】：國(guó)家自然科學(xué)基金(No:81170998) 廣東省醫(yī)學(xué)科研基金(No:C2012034)
【分類號(hào)】：R782.2
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本文編號(hào)：2508760