發(fā)布時(shí)間：2019-05-11 02:46

【摘要】：目的:體外觀察不同光敏劑濃度和不同激光能量密度的TBO-PDT抑制離體牙根管內(nèi)糞腸球菌的效果,確定最佳光敏劑參數(shù)和激光參數(shù),并在此基礎(chǔ)上探討TBO-PDT在抑制離體牙根管內(nèi)糞腸球菌上的作用優(yōu)勢(shì),為PDT應(yīng)用于根管消毒的臨床實(shí)踐提供理論與實(shí)驗(yàn)依據(jù)。方法:對(duì)離體牙進(jìn)行根管預(yù)備,接種糞腸球菌,厭氧培養(yǎng)21d后建立糞腸球菌感染根管模型。將其隨機(jī)分組:A組為陰性對(duì)照組,以生理鹽水沖洗根管;B組為陽(yáng)性對(duì)照組,以5.25%NaClO沖洗根管;其余組別根據(jù)光敏劑濃度的不同,分為C1組80μg/mL、D1組90μg/mL、E1組100μg/mL、F1組110μg/mL、G1組120μg/mL,避光孵育5min后用波長(zhǎng)為630nm功率密度為90mW/cm2半導(dǎo)體激光在根管內(nèi)上下提拉螺旋照射90s;根據(jù)光照時(shí)間的不同,分為C2組30s、D2組60s、E2組90s、F2組120s、G2組150s,光敏劑濃度為100μg/mL避光孵育5min后,用波長(zhǎng)為630nm功率密度為90mW半導(dǎo)體激光在根管內(nèi)上下提拉螺旋照射各實(shí)驗(yàn)組牙標(biāo)本,實(shí)驗(yàn)處理前后即刻取樣,厭氧培養(yǎng)24h,計(jì)數(shù)平板菌落,明確實(shí)驗(yàn)處理前后根管內(nèi)糞腸球菌的數(shù)量變化。結(jié)果:(1)不同光敏劑濃度下TBO-PDT對(duì)根管內(nèi)糞腸球菌抑菌效果的影響。平板菌落計(jì)數(shù)結(jié)果顯示:不同光敏劑濃度的各組抑菌率隨光敏劑濃度的增加而增高。與陰性對(duì)照組相比,C1組、D1組、E1組、F1組和G1組的抑菌率顯著提高,而F1組和G1組兩組間抑菌率沒(méi)有明顯差異。(2)不同光照時(shí)間對(duì)TBO-PDT抑制實(shí)驗(yàn)根管內(nèi)以生物膜形式存在的糞腸球菌的抑菌效果。平板菌落計(jì)數(shù)結(jié)果顯示:不同光照時(shí)間的各組抑菌率隨光照時(shí)間延長(zhǎng)而增高。與陰性對(duì)照組相比,其中C2組、D2組、E2組、F2組和G2組的抑菌率明顯升高,而F2組和G2組兩組間抑菌率沒(méi)有明顯差異。結(jié)論:TBO-PDT對(duì)以生物膜形式存在的糞腸球菌有明顯的抑制作用,實(shí)驗(yàn)證實(shí)TBO-PDT最佳光敏劑濃度為110μg/mL,最佳光照時(shí)間為120s。
[Abstract]:Objective: to observe the inhibitory effect of TBO-PDT with different photosensitizer concentration and different laser energy density on Enterococcus faecalis in vitro, and to determine the best photosensitizer parameters and laser parameters. On this basis, the advantages of TBO-PDT in inhibiting Enterococcus faecalis in isolated root canal were discussed, which provided theoretical and experimental basis for the clinical practice of PDT in root canal disinfection. Methods: the root canal model of Enterococcus faecalis infection was established after root canal preparation of isolated teeth and inoculated with Enterococcus faecalis for 21 days. The root canals were randomly divided into two groups: group A as negative control group, group B as positive control group, group A as negative control group, group B as positive control group, and group B as positive control group. The other groups were divided into C1 group 80 渭 g / mL,D1 group 90 渭 g / mL,E1 group 100 渭 g / mL,F1 group 110 渭 g / mL,G1 group 120 渭 g / mL, according to the concentration of photosensitizer. After 5min was incubated with light, 630nm power density was 90mW/cm2 semiconductor laser to irradiate 90 s in root canal with upper and lower Czochralski helix. According to the different light time, they were divided into C2 group (30s), D2 group (60s), E2 group (90s), F2 group (120s) and G2 group (150s). The concentration of photosensitizer was 100 渭 g / mL to avoid light to incubate 5min. The dental specimens of each experimental group were irradiated with 90mW semiconductor laser at the power density of 90mW in the root canal. The samples were sampled immediately before and after the experimental treatment and cultured for 24 hours, and the plate colonies were counted. The quantitative changes of Enterococcus faecalis in root canal before and after experimental treatment were determined. Results: (1) the effect of TBO-PDT on the bacteriostatic effect of Enterococcus faecalis in root canal under different photosensitizer concentrations. The results of plate colony count showed that the bacteriostatic rate of each group with different photosensitizer concentration increased with the increase of photosensitizer concentration. Compared with the negative control group, the bacteriostatic rate of C1 group, D1 group, E1 group, F1 group and G1 group was significantly increased. However, there was no significant difference in bacteriostatic rate between F1 group and G _ 1 group. (2) the bacteriostatic effect of different light time on Enterococcus faecalis in the form of biofilm in root canal of TBO-PDT inhibition test. The results of plate colony count showed that the bacteriostatic rate of each group increased with the prolongation of light time. Compared with the negative control group, the bacteriostatic rate of C2 group, D2 group, E2 group, F2 group and G2 group was significantly higher than that of negative control group, but there was no significant difference between F2 group and G2 group. Conclusion: TBO-PDT has obvious inhibitory effect on Enterococcus faecalis in the form of biofilm. The results show that the optimum photosensitizer concentration of TBO-PDT is 110 渭 g / mL, and the optimum light time is 120 s.
【作者單位】： 承德醫(yī)學(xué)院附屬醫(yī)院口腔科;
【分類號(hào)】：R781.05

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