發(fā)布時間：2019-03-13 13:47

【摘要】：目的:探討大蒜油聯(lián)合順鉑對人腺樣囊性癌細(xì)胞株ACC-M細(xì)胞增殖、周期及凋亡的影響，為大蒜油、順鉑聯(lián)合用于臨床防治腺樣囊性癌提供理論基礎(chǔ)和實驗依據(jù)。 方法:實驗分大蒜油組、順鉑組、大蒜油聯(lián)合順鉑組(1:1)，以未用藥物處理的ACC-M細(xì)胞為空白對照組。人腺樣囊性癌ACC-M細(xì)胞進(jìn)行體外培養(yǎng)。MTT法檢測2、4、8、16、32μg/mL大蒜油、順鉑、大蒜油聯(lián)合順鉑作用4、8、12h后人腺樣囊性癌細(xì)胞株ACC-M體外生長抑制情況；流式細(xì)胞儀檢測2、8、32μg/mL大蒜油、順鉑、大蒜油聯(lián)合順鉑作用4、8h后人腺樣囊性癌細(xì)胞株ACC-M的細(xì)胞周期分布和凋亡率情況。 結(jié)果:①隨著藥物濃度增加，各藥物組對ACC-M細(xì)胞的體外增殖抑制率明顯上升(P＜0.01)；32μg/mL聯(lián)合組作用12h效果最佳，抑制率為77.16%。大蒜油和順鉑聯(lián)合用藥組抑制癌細(xì)胞生長作用明顯強(qiáng)于單一用藥組(P＜0.01)。②大蒜油、順鉑、大蒜油聯(lián)合順鉑能使ACC-M細(xì)胞發(fā)生周期分布變化：G0/G1期細(xì)胞比例下降，G2/M期細(xì)胞比例上升，，S期細(xì)胞則無明顯變化，呈濃度依賴性(P＜0.01)。與單獨用藥組相比，32μg/mL聯(lián)合組作用8h作用后G2/M期阻滯更明顯(P＜0.01)。③隨藥物濃度增高，大蒜油、順鉑、大蒜油聯(lián)合順鉑誘導(dǎo)ACC-M細(xì)胞凋亡率增高，32μg/mL聯(lián)合組作用8h凋亡率達(dá)7.64%。聯(lián)合用藥組凋亡率明顯高于大蒜油和順鉑單獨應(yīng)用組(P＜0.01)。 結(jié)論:①2、4、8、16、32μg/mL大蒜油、順鉑、大蒜油聯(lián)合順鉑作用4、8、12h對ACC-M細(xì)胞有體外生長抑制作用，存在一定的濃度依耐性；32μg/mL大蒜油聯(lián)合順鉑作用12h抑制增殖作用明顯。②2、8、32μg/mL大蒜油、順鉑、大蒜油聯(lián)合順鉑作用4、8h能誘導(dǎo)ACC-M細(xì)胞株凋亡并阻滯細(xì)胞于G2/M期，隨藥物濃度增加，凋亡率上升，周期分布更明顯，32μg/mL大蒜油聯(lián)合順鉑作用8h效果教佳。③大蒜油聯(lián)合順鉑應(yīng)用對ACC-M細(xì)胞的作用強(qiáng)于大蒜油、順鉑單獨應(yīng)用。
[Abstract]:Aim: to investigate the effects of garlic oil combined with cisplatin on the proliferation, cell cycle and apoptosis of human adenoid cystic carcinoma cell line ACC-M in order to provide theoretical and experimental basis for the clinical use of garlic oil and cisplatin in the prevention and treatment of adenoid cystic carcinoma. Methods: the experiment was divided into three groups: garlic oil group, cisplatin group, garlic oil combined with cisplatin group (1:1). ACC-M cells were taken as blank control group. Human adenoid cystic carcinoma ACC-M cells were cultured in vitro. The growth inhibition of human adenoid cystic carcinoma cell line ACC-M was detected by MTT assay after 2,4,8,16,32 渭 g / mL garlic oil, cisplatin, garlic oil combined with cisplatin for 4, 8, 12 hours. Flow cytometry was used to detect the cell cycle distribution and apoptosis rate of human adenoid cystic carcinoma cell line ACC-M after 2,8,32 渭 g / mL garlic oil, cisplatin and garlic oil combined with cisplatin for 4 h. Results: 1 with the increase of drug concentration, the inhibition rate of proliferation of ACC-M cells in vitro increased significantly in each drug group, and the inhibition rate was 77.16% in 32 渭 g / mL group for 12 h. The inhibitory effect of garlic oil combined with cisplatin on the growth of cancer cells was significantly stronger than that of single drug group (P < 0.01). 2 garlic oil and cisplatin were found to be more effective in inhibiting the growth of cancer cells (P < 0.01). Garlic oil combined with cisplatin could change the cell cycle distribution of ACC-M cells: the proportion of cells in G0/G1 phase decreased, the proportion of cells in G2 / M phase increased, but the cells in S phase showed no significant change in a concentration-dependent manner (P < 0. 01). The G 2 / M phase arrest was more obvious in 32 渭 g / mL group than that in control group (P < 0 01). 3 the apoptosis rate of ACC-M cells induced by garlic oil, cisplatin and garlic oil combined with cisplatin increased with the increase of drug concentration. The apoptosis rate was 7.64% in 32 渭 g / mL group for 8 h. The apoptosis rate in combination group was significantly higher than that in garlic oil and cisplatin alone group (P < 0.01). Conclusion: 12, 4, 8, 16, 32 渭 g / mL garlic oil, cisplatin, garlic oil combined with cisplatin can inhibit the growth of ACC-M cells in vitro at 4, 8, 12 h, and there is a certain concentration-dependent tolerance. The inhibitory effect of garlic oil (32 渭 g / mL) combined with cisplatin for 12 h was obvious. 22, 8, 32 渭 g / mL garlic oil, cisplatin and garlic oil combined with cisplatin for 4 h and 8 h could induce apoptosis of ACC-M cell line and block cells in G 2 / M phase, with the increase of drug concentration. The apoptosis rate increased and the cycle distribution was more obvious. The effect of combination of 32 渭 g / mL garlic oil and cisplatin for 8 h was better. 3 the effect of garlic oil combined with cisplatin on ACC-M cells was stronger than that of garlic oil, and cisplatin was used alone.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.8

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