【摘要】：目的:在樹脂加強型玻璃離子(RMGI)粘結(jié)劑中添加不同含量(質(zhì)量比0%,2%,5%,10%)納米銀(NAg)抗菌劑,測量并觀察復(fù)合粘結(jié)劑銀離子的釋放濃度以及其體外防脫礦能力。方法:1測量不同質(zhì)量比復(fù)合粘結(jié)劑銀離子的釋放濃度:將不同含量NAg/RMGI(質(zhì)量比0%,2%,5%,10%)混合并充分研磨,按照廠商推薦的方法調(diào)拌,制成直徑為4mm,厚度為2mm的形狀,用光固化燈照射20s使其固化,制成NAg/RMGI試驗樣本。按照添加的NAg質(zhì)量比不同分成四組,每組18個,共計72個樣本。加入10ml去離子水中浸泡,分別于1天、3天、7天、14天、35天、42天時,利用原子吸收分光度法測量各組復(fù)合粘結(jié)劑在去離子水中的銀離子釋放情況。對實驗結(jié)果進行統(tǒng)計學(xué)處理與分析。2鏡下觀察不同質(zhì)量比復(fù)合粘結(jié)劑的防脫礦能力:將75顆人離體雙尖牙隨機分為五組,以不同NAg/RMGI質(zhì)量比調(diào)和粘接金屬托槽(杭州新亞),每組15顆。五組分別為:1組:對照組,用京津釉質(zhì)粘合劑粘接托槽;2組:樹脂加強型玻璃離子粘接托槽;3組:添加2%納米銀樹脂加強型玻璃離子粘接托槽;4組:添加5%納米銀樹脂加強型玻璃離子粘接托槽;5組:添加10%納米銀樹脂加強型玻璃離子粘接托槽。京津釉質(zhì)粘合劑組:用氣槍沖洗吹干牙面,35%磷酸酸蝕60秒,用大量的清水沖洗吹干牙面,牙面呈白堊色。涂封閉劑,用京津釉質(zhì)粘結(jié)劑粘接托槽,定位后去除托槽周圍多余的粘結(jié)劑;樹脂加強型玻璃離子粘合劑組:用氣槍沖洗吹干牙面,35%磷酸酸蝕60秒,再次清水沖洗牙面,用潤濕的小棉球沾干。按產(chǎn)品說明書推薦的粉液比調(diào)和樹脂加強型玻璃離子粘結(jié)劑粘接托槽,定位后去除托槽周圍多余的粘結(jié)劑;納米銀樹脂加強型玻璃離子組:分別按2%、5%、10%的比例混合NAg及RMGI粘結(jié)劑,充分研磨,按產(chǎn)品說明書粘接托槽,定位后用探針將托槽周圍多余的粘結(jié)劑去除。所有離體牙按組別做好標(biāo)記,粘結(jié)后30分鐘,用指甲油在托槽四圍lmm外釉質(zhì)表面開窗,指甲油完全干燥后開始體外PH循環(huán),在人工脫礦液(PH值4.5)中脫礦1.5小時后取出,去離子水沖洗干凈,然后將各組牙分別放入裝有30m1人工唾液(PH值7.0)的密閉塑料容器中37℃恒溫下保存。每天循環(huán)兩次,共循環(huán)30天。去除托槽和牙表面的粘結(jié)劑后,在掃描電鏡下觀察托槽粘結(jié)底板及周圍lmm區(qū)域的釉質(zhì)表面的形態(tài)。結(jié)果:1粘結(jié)劑銀離子的釋放:樹脂加強型玻璃離子組自始至終沒有測到銀離子的存在。第1天時2%NAg,5%NAg和10%NAg之間的銀離子釋放量沒有明顯的區(qū)別(P0.05),3~7天時銀離子釋放出現(xiàn)快速的增長,尤其以第7天最多,并且隨著加入NAg含量的增加,銀離子的釋放濃度也相應(yīng)增加,與2%NAg,5%NAg相比較,10%NAg釋放了大量的銀離子。釋放速度隨時間的推移而減緩,從第14天以后抗菌粘結(jié)劑中銀離子釋放量基本持平,且各組材料銀釋放的方式是類似的初期的大量釋放和較長時間的低水平持續(xù)釋放。2 SEM表面觀:對照組京津釉質(zhì)粘結(jié)劑組牙釉質(zhì)表面可見釉間質(zhì)溶解的多孔性組織改變,呈蜂窩狀凹陷;樹脂加強型玻璃離子和2%NAg組間牙釉質(zhì)表面較平整,可見淺表樣凹坑,有少許沉積物,兩組間沒有明顯區(qū)別。5%NAg,10%NAg組牙釉質(zhì)表面光滑平整呈魚鱗樣外觀,可見沉積樣外觀,兩組間沒有明顯區(qū)別。結(jié)論:1銀離子可以從復(fù)合納米銀樹脂加強型玻璃離子中釋放到周圍環(huán)境中,釋放量與加入抗菌劑的量成正相關(guān),釋放速度隨時間的推移而減緩。2樹脂加強型玻璃離子中加入一定量的納米銀抗菌劑,在人工脫礦環(huán)境中抑制牙釉質(zhì)脫礦作用增強。
[Abstract]:Objective: To add nano-silver (NAg) antibacterial agent with different content (0%, 2%, 5%, 10%) in the resin reinforced glass ion (RMGI) adhesive, and to measure and observe the release concentration of silver ion and its anti-demineralization ability in vitro. The method comprises the following steps of: 1, measuring the release concentration of the silver ions of the composite bonding agent with different mass ratio: mixing the different content of NAg/ RMGI (mass ratio of 0%, 2%, 5%, 10%) and grinding, mixing according to the method recommended by the manufacturer, and making into a shape with a diameter of 4 mm and a thickness of 2 mm, The photo-curing lamp was used to irradiate the 20s to cure it to prepare the NAg/ RMGI test sample. A total of 72 samples were divided into four groups, 18 in each group, according to the added NAg mass ratio. adding 10ml of deionized water, soaking in deionized water for 1 day, 3 days, 7 days, 14 days, 35 days and 42 days respectively, and using the atomic absorption spectrophotometry to measure the silver ion release condition of each group of composite adhesive in the deionized water. The results of the experiment were statistically processed and analyzed. The anti-demineralization ability of the composite adhesive with different mass ratio was observed under the mirror. The 75 people were randomly divided into five groups from the double-pointed teeth of the body, and the metal supporting groove (Hangzhou Xinya) was mixed with different NAg/ RMGI mass ratio, and 15 in each group. The five groups were: 1 group: control group, adhesion support groove with Beijing-Tianjin enamel adhesive; group 2: resin reinforced glass ionomer cement tray; group 3: addition of 2% nano-silver resin reinforced glass ionomer cement tray; group 4: addition of 5% nano-silver resin reinforced glass ionomer cement tray; 5 groups: 10% nano-silver resin reinforced glass ionomer cement tray was added. Beijing-Tianjin enamel adhesive group: the tooth surface is dried by using air gun, and the surface of the tooth surface is dried by a large amount of clean water for 60 seconds, and the tooth surface is in the chalk color. coating the sealing agent, bonding the tray with the Beijing-Tianjin enamel adhesive, and removing the excess adhesive around the tray after being positioned; and a resin reinforced glass ion adhesive group, which is used for washing and drying the tooth surface with an air gun, the 35 percent of the phosphoric acid is etched for 60 seconds, the tooth surface is washed with clear water, and the wet cotton ball is dipped with the wet cotton ball. and the nano-silver resin reinforced glass ion group is mixed with the NAg and the RMGI adhesive in a proportion of 2 percent, 5 percent and 10 percent, respectively, and is fully ground, The tray shall be bonded according to the product specification, and the excess adhesive around the tray shall be removed with the probe after positioning. All the off-body teeth are marked according to the group, 30 minutes after the bonding, the enamel surface is opened with nail polish on the periphery of the support groove, the nail polish is completely dried, the in-vitro PH circulation is started, the ore is removed for 1 to 5 hours in the artificial demineralization liquid (PHvalue 4.5), and the deionized water is washed clean, The groups of the teeth were then stored at a constant temperature of 37.degree. C.in a closed plastic container with 30ml of artificial saliva (pH 7.0), respectively. Cycle twice a day for 30 days. After removal of the adhesive on the tray and the surface of the teeth, the morphology of the surface of the enamel was observed under the scanning electron microscope. Results: 1 The release of silver ion of the binder: the presence of silver ion was not measured from the beginning to the end of the resin reinforced glass ion group. The release of silver ion between 2% NAg, 5% NAg and 10% NAg at day 1 was not significantly different (P0.05). The release of silver ions at 3-7 days showed a rapid increase, especially at the 7th day, and the release concentration of silver ions increased correspondingly with the increase of the content of the addition of NAg, compared with 2% NAg, 5% NAg. 10% of the nag released a large amount of silver ions. the release rate is slowed over time, and the amount of silver ion release in the antibacterial binder is substantially flat after day 14, and the manner of silver release in each group is a similar initial mass release and a low level of sustained release for a long time. In the control group, the enamel surface of the enamel adhesive group of the Beijing-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin The enamel surface of the 10% NAg group was smooth and flat, and the appearance of the fish scale was observed. There was no obvious difference between the two groups. conclusion: 1 silver ion can be released into the surrounding environment from the reinforced glass ion of the composite nano-silver resin, the release amount is positively correlated with the amount of the addition of the antibacterial agent, the release rate is reduced over time, and a certain amount of nano-silver antibacterial agent is added to the resin-reinforced glass ion, and the effect of demineralization of the enamel is inhibited in the artificial demineralization environment.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R783.5

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