【摘要】：目的:探索人牙髓細(xì)胞條件培養(yǎng)液(HDPSCs-CM)對人牙囊細(xì)胞(HDFSCs)向成骨分化的作用。方法:利用膠原酶消化法獲得HDFSCs,經(jīng)純化鑒定后培養(yǎng)于HDPSCs-CM中;CCK-8檢測HDFSCs增殖活性,觀察細(xì)胞形態(tài)改變;堿性磷酸酶(ALP)染色、茜素紅S染色定性分析細(xì)胞成骨能力的改變。qRT-PCR檢測HDFSCs中骨膜蛋白(POSTN)、Ⅰ型膠原(Col-Ⅰ)、堿性磷酸酶(ALP)、骨涎蛋白(BSP)以及骨橋蛋白(OPN)的mRNA表達(dá)情況。結(jié)果:經(jīng)HDPSCs-CM誘導(dǎo)后,HDFSCs形態(tài)發(fā)生成牙骨質(zhì)或成骨樣改變;誘導(dǎo)組HDFSCs增殖活性受到明顯抑制(P0.05或P0.01);誘導(dǎo)組ALP染色強(qiáng)于對照組,茜素紅S染色礦化結(jié)節(jié)多于對照組;誘導(dǎo)組POSTN、Col-Ⅰ、ALP、BSP、OPN的表達(dá)量明顯增高(P0.05或P0.01)。結(jié)論:HDPSCs-CM能促進(jìn)HDFSCs成骨分化。
[Abstract]:Aim: to investigate the effect of human dental pulp conditioned medium (HDPSCs-CM) on the osteogenic differentiation of human dental follicle cells (HDFSCs). Methods: HDFSCs, was purified and identified by collagenase digestion and cultured in HDPSCs-CM. The proliferative activity of HDFSCs was detected by CCK-8 and the changes of cell morphology were observed. Alkaline phosphatase (ALP) staining and alizarin red S staining were used to qualitatively analyze the changes of osteogenesis. QRT-PCR detection of (POSTN), 鈪，

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