【摘要】：目的:對比使用軟骨上清液和轉(zhuǎn)化生長因子兩種方法誘導(dǎo)滑膜間充質(zhì)干細(xì)胞向軟骨細(xì)胞分化。方法:分別采用消化法獲取SD大鼠滑膜間充質(zhì)干細(xì)胞和軟骨細(xì)胞。體外擴(kuò)增。實(shí)驗(yàn)組一:通過軟骨細(xì)胞上清液誘導(dǎo)滑膜間充質(zhì)干細(xì)胞向軟骨方向分化。實(shí)驗(yàn)組二:通過軟骨誘導(dǎo)液(TGF-β1,ITS+Premix,2-磷酸抗壞血酸等)誘導(dǎo)滑膜間充質(zhì)干細(xì)胞向軟骨方向分化。培養(yǎng)21d后通過形態(tài)學(xué)、免疫組織化學(xué)法檢測其生物學(xué)特性,RT-PCR檢測誘導(dǎo)后產(chǎn)物Ⅱ型膠原RNA含量。結(jié)果:2種誘導(dǎo)方法均能誘導(dǎo)滑膜間充質(zhì)干細(xì)胞成軟骨細(xì)胞方向分化。在形態(tài)學(xué)可見2組誘導(dǎo)后產(chǎn)物成軟骨樣結(jié)構(gòu),呈乳白色,質(zhì)地韌。免疫組織化學(xué)鑒定基質(zhì)能被Ⅱ型膠原染色,細(xì)胞染色呈現(xiàn)棕黃色。RT-PCR結(jié)果顯示誘導(dǎo)后的微團(tuán)表達(dá)軟骨特異性基因Ⅱ型膠原和蛋白聚糖。結(jié)論:兩組實(shí)驗(yàn)組均能誘導(dǎo)滑膜間充質(zhì)干細(xì)胞向軟骨方向分化。對比兩種實(shí)驗(yàn)方法,使用上清液誘導(dǎo)滑膜間充質(zhì)干細(xì)胞向軟骨方向分化能力更強(qiáng)。
[Abstract]:Aim: to induce the differentiation of synovial mesenchymal stem cells into chondrocytes by using cartilage supernatant and transforming growth factor. Methods: SD rat synovial mesenchymal stem cells and chondrocytes were obtained by digestion. In vitro amplification. Experimental group 1: synovial mesenchymal stem cells were induced to differentiate into cartilage by chondrocyte supernatant. Experiment 2: chondrogenic differentiation of synovial mesenchymal stem cells into cartilage was induced by chondrocyte inducing fluid (TGF- 尾 1 its Premix,2- ascorbic acid). After 21 days of culture, the biological characteristics were detected by morphological and immunohistochemical methods, and the content of type 鈪，

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