【摘要】：目的:探討α-catulin基因?qū)θ松圜[狀細(xì)胞癌(TSCC)TSCCA細(xì)胞增殖和凋亡的影響,闡明α-catulin在TSCCA細(xì)胞生物學(xué)行為中的作用。方法:取對(duì)數(shù)生長(zhǎng)期的TSCCA細(xì)胞,分為α-catulin-siRNA轉(zhuǎn)染組、空質(zhì)粒轉(zhuǎn)染組和空白對(duì)照組。RT-PCR法檢測(cè)各組TSCCA細(xì)胞中α-catulin mRNA相對(duì)表達(dá)水平;Western blotting法檢測(cè)各組TSCCA細(xì)胞中α-catulin蛋白的相對(duì)表達(dá)水平,MTT法檢測(cè)細(xì)胞生長(zhǎng)抑制率,流式細(xì)胞術(shù)檢測(cè)各組TSCCA細(xì)胞凋亡率。結(jié)果:RT-PCR檢測(cè),與空質(zhì)粒轉(zhuǎn)染組和空白對(duì)照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細(xì)胞中α-catulin mRNA相對(duì)表達(dá)水平降低(P0.01),且隨著時(shí)間的相對(duì)延長(zhǎng),α-catulin mRNA相對(duì)表達(dá)水平逐漸降低,72h時(shí)其相對(duì)表達(dá)水平低于24和48h時(shí)(P0.01)。Western blotting法檢測(cè),與空質(zhì)粒轉(zhuǎn)染組和空白對(duì)照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細(xì)胞中α-catulin蛋白相對(duì)表達(dá)水平降低(P0.05)。MTT法檢測(cè),與空質(zhì)粒轉(zhuǎn)染組和空白對(duì)照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細(xì)胞生長(zhǎng)抑制率升高(P0.001)。流式細(xì)胞術(shù)檢測(cè),與空質(zhì)粒對(duì)照組和空白對(duì)照組比較,α-catulin-siRNA轉(zhuǎn)染組TSCCA細(xì)胞凋亡率升高(P0.001)。結(jié)論:特異性沉默α-catulin基因的表達(dá)可抑制TSCC的TSCCA細(xì)胞的增殖,促進(jìn)其凋亡。
[Abstract]:Aim: to investigate the effect of 偽-catulin gene on proliferation and apoptosis of human tongue squamous cell carcinoma (TSCC) TSCCA) cells and to elucidate the role of 偽-catulin in the biological behavior of TSCCA cells. Methods: TSCCA cells in logarithmic growth stage were divided into 偽 catulin-siRNA transfection group, empty plasmid transfection group and blank control group. RT-PCR assay was used to detect the relative expression of 偽 catulin mRNA in TSCCA cells. The relative expression of 偽-catulin protein in TSCCA cells was detected by Western blotting assay, the cell growth inhibition rate was detected by MTT assay, and the apoptosis rate of TSCCA cells in each group was detected by flow cytometry. Results: compared with the blank plasmid transfection group and the blank control group, the relative expression level of 偽-catulin mRNA in the TSCCA cells of the 偽-catulin-siRNA transfection group was decreased (P0.01), and the relative expression of 偽-catulin mRNA was prolonged with time. The relative expression level of 偽-catulin mRNA decreased gradually, and was lower than that at 24 and 48 h at 72 h (P0.01). Western blotting method), compared with the blank plasmid transfection group and the blank control group. The relative expression level of 偽-catulin protein in TSCCA cells was decreased in 偽-catulin-siRNA transfection group (P0.05). MTT method). Compared with empty plasmid transfection group and blank control group, the growth inhibition rate of TSCCA cells in 偽-catulin-siRNA transfection group was higher than that in blank control group (P0. 001). Compared with empty plasmid control group and blank control group, the apoptosis rate of TSCCA cells in 偽-catulin-siRNA transfection group was higher than that in blank control group (P0. 001). Conclusion: specifically silencing the expression of 偽-catulin gene can inhibit the proliferation of TSCC TSCCA cells and promote its apoptosis.
【作者單位】： 遼寧醫(yī)學(xué)院附屬第一醫(yī)院口腔科;
【基金】：遼寧省科技廳自然科學(xué)基金資助課題(2015020333)
【分類號(hào)】：R739.86

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相關(guān)期刊論文 前2條

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本文編號(hào)：2357410