【摘要】：目的通過(guò)建立長(zhǎng)期飲用碳酸飲料大鼠正畸牙移動(dòng)實(shí)驗(yàn)動(dòng)物模型,檢測(cè)大鼠不同時(shí)期血液中的鈣、磷及堿性磷酸酶含量,脛骨近端骨密度(bone mass density,BMD),上頜第一磨牙移動(dòng)距離;同時(shí)觀測(cè)正畸牙移動(dòng)大鼠牙周組織內(nèi)血小板衍生生長(zhǎng)因子-AA(platelet-derived growth factor-AA,PDGF-AA)的表達(dá)情況。探討長(zhǎng)期飲用碳酸飲料對(duì)正畸牙移動(dòng)及牙周改建的影響,為有長(zhǎng)期飲用碳酸飲料習(xí)慣的患者正畸治療提供實(shí)驗(yàn)依據(jù)。方法SD雌性大鼠48只,2月齡,體重210±15g,清潔級(jí),普通飼料飼養(yǎng),動(dòng)物自由飲水?dāng)z食。將48只大鼠隨機(jī)分為兩組:對(duì)照組和實(shí)驗(yàn)組,分別24只。將兩組大鼠根據(jù)加力天數(shù)又隨機(jī)分為0天(0d)、7天(7d)、14天(14d)、21天(21d)四小組,分別6只。對(duì)照組大鼠給予水喂養(yǎng)的同時(shí)實(shí)驗(yàn)組給予定量的碳酸飲料喂養(yǎng),3個(gè)月后為各組大鼠安裝正畸牙移動(dòng)裝置,分別于當(dāng)天的同一時(shí)間段內(nèi)處死大鼠,腹主動(dòng)脈采血檢測(cè)血Ca、血P及ALP含量,分離左側(cè)脛骨測(cè)定BMD,量取正畸磨牙近中移動(dòng)的距離。離斷右側(cè)上頜第一磨牙及牙周組織塊制成組織學(xué)切片,利用HE染色觀察牙周組織的形態(tài)結(jié)構(gòu)變化。采用免疫組化對(duì)切片染色,利用IPP6.0軟件包半定量分析第一磨牙牙周張力側(cè)PDGF-AA表達(dá)情況,采用統(tǒng)計(jì)學(xué)方法分析PDGF-AA表達(dá)的平均光密度值(mean optical density,MOD)。結(jié)果1與對(duì)照組大鼠相比,實(shí)驗(yàn)組大鼠血Ca、ALP含量及BMD降低,血P含量升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05);隨時(shí)間的延長(zhǎng),兩組大鼠血Ca含量逐漸降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。2在不同時(shí)間段內(nèi)實(shí)驗(yàn)組的第一磨牙移動(dòng)距離均高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。隨著加力的時(shí)間延長(zhǎng),兩組第一磨牙移動(dòng)距離逐步增大,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。3免疫組化結(jié)果表明,兩組大鼠牙周組織中可見(jiàn)PDGF-AA陽(yáng)性表達(dá),主要在牙周膜成纖維細(xì)胞(periodontal ligament fibroblasts,PDLF)、成骨細(xì)胞(osteoblast,OB)、血管內(nèi)皮細(xì)胞(vascular endohtelial cell,VEC)的胞漿中出現(xiàn)。4實(shí)驗(yàn)組大鼠牙周牽張側(cè)PDGF-AA的表達(dá)均低于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。施力后兩組大鼠的牙周牽張側(cè)PDGF-AA表達(dá)逐步加強(qiáng),在7d到達(dá)高峰,隨后逐步下降,有統(tǒng)計(jì)學(xué)差別(P0.05)。結(jié)論1實(shí)驗(yàn)證實(shí)長(zhǎng)期飲用碳酸飲料大鼠的正畸牙近中移動(dòng)距離大于正常大鼠;隨時(shí)間延長(zhǎng),移動(dòng)距離增加。提示臨床考慮加力值的問(wèn)題。2長(zhǎng)期飲用碳酸飲料大鼠牙周牽張側(cè)PDGF-AA的表達(dá)低于正常大鼠,提示PDGF-AA刺激骨修復(fù)和成骨作用降低,長(zhǎng)期飲用碳酸飲料會(huì)對(duì)牙周組織骨改建造成負(fù)面影響。
[Abstract]:Objective to establish a rat model of orthodontic tooth movement after drinking carbonated drinks for a long time, and to detect the contents of calcium, phosphorus and alkaline phosphatase, and bone mineral density (bone mass density,BMD) of proximal tibia. Maxillary first molar movement distance; The expression of platelet-derived growth factor AA (platelet-derived growth factor-AA,PDGF-AA) in periodontal tissues of orthodontic rats was also observed. To investigate the effects of long-term drinking carbonated drinks on orthodontic tooth movement and periodontal remodeling, and to provide experimental evidence for orthodontic treatment of patients with long-term drinking carbonated drinks. Methods 48 SD female rats, 2 months old, weighing 210 鹵15 g, were fed with common feed and fed freely. Forty-eight rats were randomly divided into two groups: control group and experimental group, 24 rats in each group. The rats in the two groups were randomly divided into four groups according to the days of exertion: 0 day (0 d), 7 day (7 d), 14 day (14 d), 21 day (21 d), and 6 rats in each group. The rats in the control group were given water feeding and the experimental group were fed with carbonated drinks. After 3 months, the rats in each group were fitted with orthodontic tooth movement device. The rats were killed at the same time of the same day. Blood samples from the abdominal aorta were collected to detect Ca,. The contents of P and ALP were measured in the left tibia, and the distance between the proximal movement of orthodontic molars was measured. The right maxillary first molar and periodontal tissue were cut into histological sections. The morphological and structural changes of periodontal tissue were observed by HE staining. The expression of PDGF-AA in the periodontal tension side of the first molar was semi-quantitatively analyzed by IPP6.0 software package, and the average optical density of PDGF-AA expression was analyzed by statistical method (mean optical density,MOD). Results 1 compared with the control group, the content of Ca,ALP and BMD in the blood of the experimental group decreased, and the content of P in the blood increased (P0.05). With the extension of time, the blood Ca content of the two groups decreased gradually, the difference was statistically significant (P0.05). 2 the first molar movement distance of the experimental group was higher than that of the control group in different time period, the difference was statistically significant (P0.05). The distance between the first molars of the two groups gradually increased with the extension of the time of applying force, the difference was statistically significant (P0.05). 3 Immunohistochemical results showed that the positive expression of PDGF-AA was found in the periodontal tissues of the two groups. It was mainly found in the cytoplasm of periodontal ligament fibroblasts (periodontal ligament fibroblasts,PDLF), osteoblasts (osteoblast,OB) and vascular endothelial cells (vascular endohtelial cell,VEC). 4 the expression of PDGF-AA in the periodontal distraction side of the experimental group was lower than that in the control group. The difference was statistically significant (P0.05). The expression of PDGF-AA in the periodontal distraction side of the two groups was gradually increased after applied, reached the peak on the 7th day, and then decreased gradually, there was statistical difference (P0.05). Conclusion 1 the proximal distance of orthodontic teeth of rats drinking carbonated drinks for a long time is longer than that of normal rats, and the distance increases with time. 2 the expression of PDGF-AA in periodontal distraction side of rats with long-term carbonated drink was lower than that in normal rats, suggesting that PDGF-AA stimulated bone repair and osteogenesis decreased. Long-term consumption of carbonated drinks can have a negative effect on periodontal bone remodeling.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R783.5

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