長期飲用碳酸飲料對(duì)大鼠正畸牙移動(dòng)影響的實(shí)驗(yàn)研究
[Abstract]:Objective to establish a rat model of orthodontic tooth movement after drinking carbonated drinks for a long time, and to detect the contents of calcium, phosphorus and alkaline phosphatase, and bone mineral density (bone mass density,BMD) of proximal tibia. Maxillary first molar movement distance; The expression of platelet-derived growth factor AA (platelet-derived growth factor-AA,PDGF-AA) in periodontal tissues of orthodontic rats was also observed. To investigate the effects of long-term drinking carbonated drinks on orthodontic tooth movement and periodontal remodeling, and to provide experimental evidence for orthodontic treatment of patients with long-term drinking carbonated drinks. Methods 48 SD female rats, 2 months old, weighing 210 鹵15 g, were fed with common feed and fed freely. Forty-eight rats were randomly divided into two groups: control group and experimental group, 24 rats in each group. The rats in the two groups were randomly divided into four groups according to the days of exertion: 0 day (0 d), 7 day (7 d), 14 day (14 d), 21 day (21 d), and 6 rats in each group. The rats in the control group were given water feeding and the experimental group were fed with carbonated drinks. After 3 months, the rats in each group were fitted with orthodontic tooth movement device. The rats were killed at the same time of the same day. Blood samples from the abdominal aorta were collected to detect Ca,. The contents of P and ALP were measured in the left tibia, and the distance between the proximal movement of orthodontic molars was measured. The right maxillary first molar and periodontal tissue were cut into histological sections. The morphological and structural changes of periodontal tissue were observed by HE staining. The expression of PDGF-AA in the periodontal tension side of the first molar was semi-quantitatively analyzed by IPP6.0 software package, and the average optical density of PDGF-AA expression was analyzed by statistical method (mean optical density,MOD). Results 1 compared with the control group, the content of Ca,ALP and BMD in the blood of the experimental group decreased, and the content of P in the blood increased (P0.05). With the extension of time, the blood Ca content of the two groups decreased gradually, the difference was statistically significant (P0.05). 2 the first molar movement distance of the experimental group was higher than that of the control group in different time period, the difference was statistically significant (P0.05). The distance between the first molars of the two groups gradually increased with the extension of the time of applying force, the difference was statistically significant (P0.05). 3 Immunohistochemical results showed that the positive expression of PDGF-AA was found in the periodontal tissues of the two groups. It was mainly found in the cytoplasm of periodontal ligament fibroblasts (periodontal ligament fibroblasts,PDLF), osteoblasts (osteoblast,OB) and vascular endothelial cells (vascular endohtelial cell,VEC). 4 the expression of PDGF-AA in the periodontal distraction side of the experimental group was lower than that in the control group. The difference was statistically significant (P0.05). The expression of PDGF-AA in the periodontal distraction side of the two groups was gradually increased after applied, reached the peak on the 7th day, and then decreased gradually, there was statistical difference (P0.05). Conclusion 1 the proximal distance of orthodontic teeth of rats drinking carbonated drinks for a long time is longer than that of normal rats, and the distance increases with time. 2 the expression of PDGF-AA in periodontal distraction side of rats with long-term carbonated drink was lower than that in normal rats, suggesting that PDGF-AA stimulated bone repair and osteogenesis decreased. Long-term consumption of carbonated drinks can have a negative effect on periodontal bone remodeling.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R783.5
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