粗糙純鈦表面摻鍶二氧化鈦層促進(jìn)骨整合的動物實(shí)驗(yàn)研究
發(fā)布時間：2018-11-14 14:07
【摘要】：種植體與骨組織之間的骨結(jié)合和良好的初期穩(wěn)定性是種植手術(shù)成功的重要基礎(chǔ)。隨著口腔種植技術(shù)的發(fā)展,種植修復(fù)的成功率有了極大的提高,然而臨床工作中患者的骨缺損情況各異,對于吸煙、牙周病、糖尿病、嚴(yán)重骨缺損的患者,單純的 SLA(Sandblasted with large grit and acid etched,大顆粒噴砂酸蝕)種植體可能無法滿足所有患者的需求。種植體的表面特征是影響骨整合速度和程度的一個重要因素。關(guān)于種植體表面的改性有很多,包括物理、化學(xué)、生物等方面的改性。近年來,由于抗骨質(zhì)疏松藥物雷尼酸鍶的使用,鍶表面改性成為研究熱點(diǎn)。本研究利用水熱處理法對SLA種植體進(jìn)行摻鍶表面改性,進(jìn)行表征實(shí)驗(yàn)和動物實(shí)驗(yàn),來探究摻鍶種植體的生物學(xué)效應(yīng),及其與表征之間的聯(lián)系,為今后種植體表面改性研究提供實(shí)驗(yàn)依據(jù)和理論基礎(chǔ)。首先將純鈦表面用不同粗細(xì)的砂紙打磨拋光,噴砂,雙重酸蝕,形成微米多孔狀表面(SLA表面)。隨后,采用課題組前期研究確立的Sr(OH)□·8H□O水熱反應(yīng)溶液的適宜濃度、溫度和時間,制備具有微納米結(jié)構(gòu)且在一定時間內(nèi)可持續(xù)釋放鍶的摻鍶鈦表面(Sr-SLA 表面)(Strontium-Sandblasted with large grit and acidetchedsurface,大顆粒噴砂酸蝕摻鍶表面)。場發(fā)射掃描電鏡結(jié)果顯示,在微米和亞微米級別上可見Sr-SLA表面和SLA表面都表現(xiàn)出由噴砂和雙重酸蝕形成的典型的相似不規(guī)則凹痕。在納米級別上,Sr-SLA表面出現(xiàn)出更多的顆粒樣聚集物和凸起,直徑約為50nm的致密納米點(diǎn),SLA表面無上述納米點(diǎn)。原子力顯微鏡結(jié)果也顯示Sr-SLA表面存在更多的凸起和隆起。表明水熱處理在一定程度上保留了鈦片經(jīng)噴砂酸蝕后形成的微米級結(jié)構(gòu),并新形成了一定的納米結(jié)構(gòu)。X射線光電子能譜和X射線衍射結(jié)果顯示,Sr-SLA表面含有鈦酸鍶,SLA表面不含鈦酸鍶。電感耦合等離子體質(zhì)譜結(jié)果顯示,Sr-SLA表面鍶釋放量隨著時間延長逐漸降低。硬度結(jié)果顯示Sr-SLA表面和SLA表面沒有明顯差異。隨后,將具有粗糙多孔純鈦摻鍶種植體(Sr-SLA種植體)和對照組種植體(SLA種植體)共計(jì)64枚種植體同期植入到16只新西蘭兔的股骨和脛骨干骺端,每只新西蘭兔植入4枚種植體,分別在植入后3周和6周處死實(shí)驗(yàn)動物,進(jìn)行扭力測試和硬組織切片分析。動物實(shí)驗(yàn)結(jié)果表明,相比于SLA種植體,粗糙多孔純鈦摻鍶種植體(Sr-SLA種植體)可促進(jìn)動物體內(nèi)早期骨形成。本研究探索水熱處理法制備的多孔純鈦摻鍶微納米表面的表征和體內(nèi)生物學(xué)效應(yīng),發(fā)現(xiàn)摻鍶種植體能夠提高種植體骨結(jié)合,為今后種植體的表面改性提供了實(shí)驗(yàn)依據(jù)和理論基礎(chǔ)。
[Abstract]:The bone bonding between implant and bone tissue and good initial stability are the important basis for the success of implant operation. With the development of dental implant technology, the success rate of implant repair has been greatly improved. However, the clinical work of patients with bone defects are different, for smoking, periodontal disease, diabetes, serious bone defect patients, SLA (Sandblasted with large grit and acid etched, large-particle acid blast implants may not be sufficient for all patients. The surface features of implants are an important factor affecting the speed and degree of bone integration. There are many modifications about implant surface, including physical, chemical, biological and so on. In recent years, strontium surface modification has become a hot topic due to the use of anti-osteoporosis drug strontium ranionate. In this study, the surface modification of strontium doped SLA implants was carried out by hydrothermal treatment to investigate the biological effects of strontium doped implants and their relationship with characterization. To provide experimental and theoretical basis for the study of implant surface modification in the future. Firstly, the pure titanium surface was polished with different thickness sandpaper, sandblasting and double acid etching to form a micron porous surface (SLA surface). Then, the suitable concentration, temperature and time of Sr (OH)-8H-O hydrothermal reaction solution were determined by our previous study. Strontium doped titanium (Sr-SLA surface) with microstructures and sustained release of strontium (Sr-SLA surface) (Sr-doped surface) with large Strontium-Sandblasted with large grit and acidetchedsurface, particles was prepared. The results of field emission scanning electron microscopy (SEM) show that both the Sr-SLA surface and the SLA surface exhibit typical similar irregular indentation formed by sand blasting and double acid etching at the micron and submicron levels. At the nanometer level, there are more granular aggregates and bulges on the surface of Sr-SLA, the diameter of which is about the dense nanowires of 50nm, but there are no such nanoparticles on the surface of SLA. Atomic force microscopy (AFM) results also show that there are more bulges and bulges on the Sr-SLA surface. The results show that the micron structure of titanium wafer formed by sandblasting acid etching is preserved in hydrothermal treatment, and a certain nanometer structure is formed. The results of X-ray photoelectron spectroscopy and X-ray diffraction show that the surface of Sr-SLA contains strontium titanate. The surface of SLA does not contain strontium titanate. The results of inductively coupled plasma mass spectrometry (ICP-MS) show that strontium emission from Sr-SLA surface decreases with time. The hardness results show that there is no obvious difference between Sr-SLA surface and SLA surface. Subsequently, 64 implants with coarse porous pure titanium strontium doped implants (Sr-SLA implants) and control group implants (SLA implants) were implanted into the femur and tibial metaphysis of 16 New Zealand rabbits simultaneously. Four implants were implanted into each New Zealand rabbit. The experimental animals were killed 3 and 6 weeks after implantation respectively. The torsion test and hard tissue analysis were performed. Compared with SLA implants, coarse porous pure titanium implants (Sr-SLA implants) can promote early bone formation. The aim of this study was to investigate the characterization and biological effects of porous pure titanium doped strontium nanocrystalline surfaces prepared by hydrothermal treatment. It was found that strontium doped implants could improve the osseous bonding of implants. It provides experimental and theoretical basis for the surface modification of implants in the future.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R783.6
本文編號：2331375
[Abstract]:The bone bonding between implant and bone tissue and good initial stability are the important basis for the success of implant operation. With the development of dental implant technology, the success rate of implant repair has been greatly improved. However, the clinical work of patients with bone defects are different, for smoking, periodontal disease, diabetes, serious bone defect patients, SLA (Sandblasted with large grit and acid etched, large-particle acid blast implants may not be sufficient for all patients. The surface features of implants are an important factor affecting the speed and degree of bone integration. There are many modifications about implant surface, including physical, chemical, biological and so on. In recent years, strontium surface modification has become a hot topic due to the use of anti-osteoporosis drug strontium ranionate. In this study, the surface modification of strontium doped SLA implants was carried out by hydrothermal treatment to investigate the biological effects of strontium doped implants and their relationship with characterization. To provide experimental and theoretical basis for the study of implant surface modification in the future. Firstly, the pure titanium surface was polished with different thickness sandpaper, sandblasting and double acid etching to form a micron porous surface (SLA surface). Then, the suitable concentration, temperature and time of Sr (OH)-8H-O hydrothermal reaction solution were determined by our previous study. Strontium doped titanium (Sr-SLA surface) with microstructures and sustained release of strontium (Sr-SLA surface) (Sr-doped surface) with large Strontium-Sandblasted with large grit and acidetchedsurface, particles was prepared. The results of field emission scanning electron microscopy (SEM) show that both the Sr-SLA surface and the SLA surface exhibit typical similar irregular indentation formed by sand blasting and double acid etching at the micron and submicron levels. At the nanometer level, there are more granular aggregates and bulges on the surface of Sr-SLA, the diameter of which is about the dense nanowires of 50nm, but there are no such nanoparticles on the surface of SLA. Atomic force microscopy (AFM) results also show that there are more bulges and bulges on the Sr-SLA surface. The results show that the micron structure of titanium wafer formed by sandblasting acid etching is preserved in hydrothermal treatment, and a certain nanometer structure is formed. The results of X-ray photoelectron spectroscopy and X-ray diffraction show that the surface of Sr-SLA contains strontium titanate. The surface of SLA does not contain strontium titanate. The results of inductively coupled plasma mass spectrometry (ICP-MS) show that strontium emission from Sr-SLA surface decreases with time. The hardness results show that there is no obvious difference between Sr-SLA surface and SLA surface. Subsequently, 64 implants with coarse porous pure titanium strontium doped implants (Sr-SLA implants) and control group implants (SLA implants) were implanted into the femur and tibial metaphysis of 16 New Zealand rabbits simultaneously. Four implants were implanted into each New Zealand rabbit. The experimental animals were killed 3 and 6 weeks after implantation respectively. The torsion test and hard tissue analysis were performed. Compared with SLA implants, coarse porous pure titanium implants (Sr-SLA implants) can promote early bone formation. The aim of this study was to investigate the characterization and biological effects of porous pure titanium doped strontium nanocrystalline surfaces prepared by hydrothermal treatment. It was found that strontium doped implants could improve the osseous bonding of implants. It provides experimental and theoretical basis for the surface modification of implants in the future.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R783.6
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 ;Fluorescence microscopic analysis of bone osseointegration of strontium-substituted hydroxyapatite implants[J];Journal of Zhejiang University-Science B(Biomedicine & Biotechnology);2012年05期
，本文編號：2331375
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