【摘要】：目的:研究犬骨髓基質(zhì)細(xì)胞(bone marrow stromal cells,BMSCs)在柚皮苷誘導(dǎo)下定向成骨及成骨分化能力。方法:抽取犬骨髓,貼壁法體外培養(yǎng),流式細(xì)胞儀鑒定,加入不同濃度柚皮苷(1×10-5、1×10-6、1×10-7、1×10-8、1×10-9 mol/L)誘導(dǎo),以CCK-8檢測藥物毒性,定量檢測堿性磷酸酶、von Kossa檢測鈣結(jié)節(jié)形成。采用SPSS20.0軟件包對數(shù)據(jù)進(jìn)行統(tǒng)計學(xué)分析。結(jié)果:經(jīng)流式細(xì)胞儀檢測,CD34、CD45低表達(dá)、CD90高表達(dá)分別為0.126%、0.075%和95.4%;柚皮苷濃度在10-6、10-7 mol/L時,能明顯促進(jìn)細(xì)胞增殖;經(jīng)柚皮苷誘導(dǎo)后,堿性磷酸酶含量顯著提高,其中,濃度為10-6 mol/L時,ALP相對值最高(P0.05);von Kossa鈣結(jié)節(jié)檢測呈陽性。結(jié)論:犬骨髓基質(zhì)細(xì)胞在柚皮苷誘導(dǎo)下能分化為成骨細(xì)胞。柚皮苷濃度為10-6 mol/L時,能明顯促進(jìn)骨髓基質(zhì)細(xì)胞的增殖及分化。
[Abstract]:Aim: to study the osteogenic and osteogenic differentiation of canine bone marrow stromal cells (bone marrow stromal cells,BMSCs) induced by naringin. Methods: canine bone marrow was extracted, cultured in vitro and identified by flow cytometry. Naringin was induced with different concentrations of naringin (1 脳 10-5, 1 脳 10-6, 1 脳 10-7, 1 脳 10-8, 1 脳 10-9 mol/L). The drug toxicity was detected by CCK-8 and calcium nodule formation was detected by alkaline phosphatase, von Kossa. The data were analyzed by SPSS20.0 software package. Results: low expression of CD34,CD45 and 0. 126% and 95. 4% of high expression of CD90 were detected by flow cytometry, naringin concentration at 10 ~ (-6) ~ 10 ~ (-7) mol/L could obviously promote cell proliferation, and the content of alkaline phosphatase was significantly increased after naringin was induced by naringin. When the concentration was 10 ~ (-6) mol/L, the relative value of ALP was the highest (P0.05); von Kossa calcium nodules were positive. Conclusion: canine bone marrow stromal cells can differentiate into osteoblasts induced by naringin. When the concentration of naringin was 10-6 mol/L, it could promote the proliferation and differentiation of bone marrow stromal cells.
【作者單位】： 同濟(jì)大學(xué)口腔醫(yī)學(xué)院口腔生物醫(yī)學(xué)及轉(zhuǎn)化醫(yī)學(xué)實驗室;同濟(jì)大學(xué)附屬東方醫(yī)院口腔科;上海交通大學(xué)醫(yī)學(xué)院免疫學(xué)教研室;
【基金】：國家自然科學(xué)基金(81070806) 上海市科學(xué)技術(shù)委員會資助項目(10JC1413300) 浦東新區(qū)衛(wèi)生局青年項目(PW2011B-13)~~
【分類號】：R782

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