【摘要】：目的： 本實驗通過建立SD大鼠的慢性牙周炎(chronic periodontitis,CP)和動脈粥樣硬化(atherosclerosis, As)模型,模擬慢性牙周炎患者臨床治療過程中可能采取的干預(yù)措施,研究不同口腔干預(yù)對CP大鼠動脈粥樣硬化發(fā)生、發(fā)展的影響。 方法： 將50只雄性SD大鼠編號后根據(jù)數(shù)字隨機(jī)表隨機(jī)分成3大組：A組(正常對照組)7只、B組(As組)8只、C組(CP組)35只,牙周炎建模成功后,再將C組按照不同口腔干預(yù)措施分為C1組(自然進(jìn)程)、C2組(單純機(jī)械治療)、C3組(藥物治療)、C4-1組(拔牙)和C4-2組(拔牙+抗生素),每組均為7只。按上述措施進(jìn)行口腔干預(yù),酶聯(lián)免疫吸附法(ELISA)檢測5個不同時間點(建模成功后1、3、5、7和9周)血清IL-6含量；建模成功后9周末處死動物,免疫組化法檢測頸動脈血管組織中MMP-2,9的含量。 結(jié)果： 1、血清學(xué)顯示：隨著時間推移,B和C1組血清IL-6含量一直呈升高趨勢,且顯著高于A組(P0.01)；各干預(yù)組(C2、C3、C4-1、C4-2組)IL-6含量逐漸升高,并在建模成功后5周達(dá)到峰值(C2:62.3±14.3、C3:58.2±8.7、C4-1:127.0±29.9、C4-2：120.6±23.1),且高于B組(43.4±7.5)(P0.01),其中C4-1和C4-2組升高最明顯,均接近B組的3倍,C2和C3組接近其1.5倍,之后逐漸下降。最終,C2組(28.6±8.1)、C3組(40.8±15.1)和C4-2組(32.7±11.1)低于B組(66.3±7.8)(P0.05),且C2組最低；C4-1組(72.8±16.4)雖下降明顯,但仍為最高。 2、免疫組化法檢測顯示：B、C1和C4-1組MMP-2,9大量陽性表達(dá),且陽性染色深度均明顯高于A組(MMP-2,9灰度值分別為：183.0±2.0、181.3±2.0),灰度值差異有統(tǒng)計學(xué)意義(P0.01),其中C4-1組陽性染色深度最強(qiáng),B和C1組依次降低(C4-1：123.1±2.9、121.0±3.2；B：126.3±2.0、124.8±2.8；C1：139.8±2.2、139.7±3.2)(P0.01)；C2、C3和C4-2組陽性染色深度均明顯低于C1組(C2：169.3±3.4、169.7±2.3；C3：148.9±1.7、145.1±2.5；C4-2：157.7±1.2、155.8±2.7)(P0.01),C2組陽性染色最弱。 結(jié)論： 1、成功建立了SD大鼠的慢性牙周炎和動脈粥樣硬化模型。 2、單純慢性牙周炎SD大鼠任其牙周炎癥自然發(fā)展,發(fā)生As的風(fēng)險可能會逐漸增高。 3、單純慢性牙周炎SD大鼠,牙周機(jī)械治療在短時間內(nèi)可能會增加As的發(fā)病風(fēng)險,但是長時間觀察,其風(fēng)險逐漸降低,對血管的改善程度效果最明顯。 4、單純慢性牙周炎SD大鼠,直接拔牙風(fēng)險較高,而拔牙后給予全身應(yīng)用抗炎藥可以明顯降低As發(fā)生、發(fā)展風(fēng)險。
[Abstract]:Objective: to establish the (atherosclerosis, As) model of chronic periodontitis (chronic periodontitis,CP) and atherosclerosis in SD rats, and to simulate the possible intervention measures in the course of clinical treatment of chronic periodontitis. To study the effect of different oral intervention on the occurrence and development of atherosclerosis in CP rats. Methods: fifty male SD rats were randomly divided into three groups: group A (normal control group), group B (As group, 8 rats) and group C (CP group, 35 rats). Group C was divided into C1 group (natural process), C2 group (simple mechanical treatment), C3 group (drug therapy), C4-1 group (extraction of teeth) and C4-2 group (extraction antibiotics) according to different oral intervention measures. Each group had 7 rats. According to the above measures, the serum IL-6 levels were measured by Elisa (ELISA) at 5 different time points (1 ~ 3 ~ 5 ~ 5 weeks after modeling), and the animals were killed at the end of 9 weeks after modeling. The content of MMP-2,9 in carotid artery was detected by immunohistochemical method. Results: 1. Serological results showed that the serum IL-6 levels in groups B and C1 increased with time and were significantly higher than those in group A (P0.01), and the content of IL-6 in each intervention group (C2C3C3-C4-1C4-2) gradually increased. The peak value of C2: 62.3 鹵14.3C3: 58.2 鹵8.7 C4-1: 127.0 鹵29.9C4-2: 12.6 鹵23.1 was higher than that of group B (43.4 鹵7.5) (P0.01). The increase of C4-1 and C4-2 was the most obvious, nearly 3 times of that of group B, 1.5 times of group C2 and C3, and then decreased gradually. Finally, C _ 2 group (28.6 鹵8.1), C _ 3 group (40.8 鹵15.1) and C _ 4-2 group (32.7 鹵11.1) were lower than B group (66.3 鹵7.8) (P0.05), and C _ 2 group (72.8 鹵16.4) was the lowest. The depth of positive staining was significantly higher in group A than in group A (MMP-2,9 gray value: 183.0 鹵2.0181.3 鹵2.0), and the difference was statistically significant (P0.01). The depth of positive staining was the strongest in group C4-1, and decreased in group B and C1 (C4-1: 123.1 鹵2.9121.0 鹵3.2Bw 126.3 鹵2.0124.8 鹵2.8124.8 鹵2.8C1w 139.8 鹵2.2139.7 鹵3.2) (P0.01). The depth of positive staining in C _ 2 C _ 3 and C _ 4-2 groups was significantly lower than that in C _ 1 group (C _ 2: 169.3 鹵3.4169.7 鹵2.3 C _ 3: 148.9 鹵1.7145.1 鹵2.5C4-2: 157.7 鹵1.2155.8 鹵2.7) (P0.01). Conclusion: 1. The models of chronic periodontitis and atherosclerosis in SD rats were successfully established. 2. Chronic periodontitis SD rats were allowed to develop their periodontitis naturally. The risk of As may increase gradually. 3. In SD rats with chronic periodontitis, periodontal mechanical therapy may increase the risk of As in a short period of time, but the risk decreases gradually after a long period of observation. (4) SD rats with chronic periodontitis had a higher risk of direct extraction of teeth, and the risk of development of As could be significantly reduced by systemic application of anti-inflammatory drugs after extraction of teeth.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R781.42

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