【摘要】：目的觀察羧基改性的聚酰胺-胺型樹(shù)枝狀大分子（PAMAM）誘導(dǎo)脫礦牙本質(zhì)的再礦化隨時(shí)間變化的情況以及Ca(OH)2溶液預(yù)處理對(duì)羧基改性的PAMAM在脫礦牙本質(zhì)再礦化中的影響。分析其再礦化的結(jié)晶體封閉牙本質(zhì)小管的程度，為臨床上預(yù)防和治療牙本質(zhì)過(guò)敏提供實(shí)驗(yàn)依據(jù)。 方法首先將2.5代羧基改性的PAMAM在脫礦的牙本質(zhì)上進(jìn)行修飾，通過(guò)衰減全反射傅里葉紅外變換光譜（ATR-FTIR）進(jìn)行表征。實(shí)驗(yàn)分為兩個(gè)部分：第一部分為探討羧基改性的PAMAM誘導(dǎo)脫礦牙本質(zhì)再礦化的時(shí)間相關(guān)性，通過(guò)掃描電鏡（SEM）在1d、3d、5d、7d、14d時(shí)觀察PAMAM誘導(dǎo)牙本質(zhì)再礦化的情況；第二部分研究Ca(OH)2溶液預(yù)處理對(duì)羧基改性的PAMAM在脫礦牙本質(zhì)再礦化中的影響。將酸蝕脫礦牙本質(zhì)模型隨機(jī)分為4個(gè)組：A組，空白對(duì)照組，未經(jīng)任何處理；B組，經(jīng)氫氧化鈣溶液預(yù)處理；C組，經(jīng)羧基改性PAMAM溶液處理；D組，經(jīng)PAMAM溶液與Ca(OH)2溶液處理。A、B、C、D組在人工唾液中礦化2周。SEM觀察樣本橫斷面以及縱斷面的結(jié)構(gòu)形態(tài)，X射線能譜法(EDS)檢測(cè)樣本表面的Ca、P離子并將Ca/P比值進(jìn)行統(tǒng)計(jì)分析，X-衍射分析（XRD）對(duì)其晶體的物相進(jìn)行分析。 結(jié)果實(shí)驗(yàn)第一部分：SEM顯示，隨著時(shí)間的延長(zhǎng)，再礦化的效果越好，再礦化2周時(shí)牙本質(zhì)表面及小管覆蓋了結(jié)晶體，未見(jiàn)開(kāi)放性牙本質(zhì)小管。實(shí)驗(yàn)第二部分：SEM顯示，A組表面可見(jiàn)大小不一的片狀結(jié)晶體，結(jié)構(gòu)混亂，牙本質(zhì)小管內(nèi)只有少量的結(jié)晶體；B組表面可見(jiàn)分布不均的結(jié)晶體，牙本質(zhì)小管呈半開(kāi)放狀態(tài)，小管內(nèi)結(jié)晶體成片狀，排列混亂；C組表面可見(jiàn)大量的分布均勻的結(jié)晶體，只有少量的開(kāi)放性牙本質(zhì)小管，小管內(nèi)結(jié)晶垂直于小管壁表面，相互平行排列；D組可見(jiàn)大量分布均勻的結(jié)晶體，未見(jiàn)開(kāi)放性的牙本質(zhì)小管，小管內(nèi)結(jié)晶體垂直于小管壁表面，，相互平行排列。A、B、C、D組的Ca/P比值分別為1.57±0.020、1.62±0.024、1.60±0.019、1.64±0.024，各組之間差別有統(tǒng)計(jì)學(xué)意義。XRD顯示A、B、D3個(gè)組生成的礦化物結(jié)晶度弱，C組礦化物結(jié)晶度強(qiáng)。 結(jié)論羧基改性的PAMAM協(xié)同Ca(OH)2溶液預(yù)處理加速促進(jìn)脫礦牙本質(zhì)的再礦化，時(shí)間越長(zhǎng)，礦化效果越明顯。在人工唾液中形成的再礦化物可以有效地封閉牙本質(zhì)小管，并與小管壁緊密結(jié)合。上述研究結(jié)果表明PAMAM在治療牙本質(zhì)過(guò)敏癥上具有良好的前景。
[Abstract]:Objective to observe the effect of carboxyl modified polyamide-amine dendrimer (PAMAM) on demineralization of demineralized dentin induced by (PAMAM) and the effect of Ca (OH) 2 solution pretreatment on decarboxylated PAMAM in demineralization of demineralized dentin. The degree of dentin tubule sealing by its mineralized crystal was analyzed to provide experimental basis for clinical prevention and treatment of dentin hypersensitivity. Methods the modified PAMAM was firstly modified on the demineralized dentin and characterized by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The experiment was divided into two parts: the first part was to investigate the temporal correlation of demineralized dentin induced by carboxyl modified PAMAM. The PAMAM induced dentin remineralization was observed by scanning electron microscope (SEM) at 1 d ~ 3 d ~ 5 d ~ 7 d ~ (14) d. In the second part, the effect of Ca (OH) _ 2 solution pretreatment on PAMAM modified by carboxyl group in demineralization of demineralized dentin was studied. The dentin model of acid etching demineralization was randomly divided into 4 groups: group A (n = 4) and control group (n = 10). Group C was pretreated with calcium hydroxide solution without any treatment, and group D was treated with carboxyl modified PAMAM solution. The samples were mineralized in artificial saliva by PAMAM solution and Ca (OH) 2 solution for 2 weeks. (EDS) was used to detect the Ca,P ion on the surface of the sample and the ratio of Ca/P was statistically analyzed. The phase of the crystal was analyzed by (XRD). Results in the first part of the experiment, SEM showed that with the extension of time, the effect of remineralization was better. After 2 weeks of mineralization, the dentine surface and tubules were covered with crystals, and no open dentine tubules were found. The second part of the experiment: SEM showed that in group A, there were flake crystals of different sizes and disordered structure. There were only a few crystals in the dentine tubule group B with uneven distribution on the surface, and the dentine tubules were semi-open. In group C, a large number of uniform crystals were found on the surface of the tubules. There were only a few open dentine tubules. The crystals in the tubules were perpendicular to the walls of the tubules and arranged in parallel with each other. In group D, a large number of uniformly distributed crystals were observed, and no open dentine tubules were found. The crystalline bodies in the tubules were perpendicular to the wall surface of the tubules. The Ca/P ratio of group D was 1.57 鹵0.020 鹵1.62 鹵0.024 鹵1.60 鹵0.019 鹵1.64 鹵0.024, respectively. The difference among groups was statistically significant. XRD showed that the crystallinity of mineralized compounds in group C was weak and the crystallinity of mineralized substance in group C was strong. Conclusion carboxyl modified PAMAM combined with Ca (OH) 2 solution pretreatment can accelerate the demineralization of demineralized dentin. The longer the time is, the more obvious the mineralization effect is. The mineralized material formed in artificial saliva can effectively seal the dentine tubules and bind to the wall of the tubules. These results suggest that PAMAM has a good prospect in the treatment of dentin hypersensitivity.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R781.2

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