【摘要】：目的探討低氧對人牙周膜干細(xì)胞(PDLSCs)骨向分化的影響。方法采用組織塊法體外分離培養(yǎng)PDLSCs,分別在常氧和低氧條件下培養(yǎng)PDLSCs,低氧組在2%O2濃度下培養(yǎng)6、12、24、48 h后檢測其堿性磷酸酶(ALP)活性,并通過熒光定量PCR檢測低氧培養(yǎng)的PDLSCs中骨鈣素(OCN)、低氧誘導(dǎo)因子1α(Hif-1α)、堿性磷酸酶(ALP)以及成骨相關(guān)基因核心結(jié)合因子(Runx-2)等基因的表達(dá)變化;通過Western blot法檢測低氧培養(yǎng)的PDLSCs中Runx-2、Hif-1α等蛋白的表達(dá)變化。結(jié)果低氧組的PDLSCs的ALP水平高于常氧組,但低氧48 h開始抑制ALP水平,熒光定量PCR和Western blot結(jié)果顯示低氧培養(yǎng)48 h內(nèi)的PDLSCs的成骨能力高于常氧組,但低氧培養(yǎng)48 h則抑制其成骨能力。結(jié)論48 h內(nèi)低氧可顯著增強(qiáng)PDLSCs骨向分化作用,48 h則開始抑制其成骨能力。
[Abstract]:Objective to investigate the effect of hypoxia on bone differentiation of human periodontal ligament stem cells (PDLSCs). Methods PDLSCs, was isolated and cultured in vitro by tissue mass method. The (ALP) activity of alkaline phosphatase (ALP) was detected in PDLSCs, hypoxia group cultured at 2%O2 concentration for 48 h after being cultured in normoxic and hypoxic conditions. The expression of osteocalcin (OCN), hypoxia inducible factor 1 偽 (Hif-1 偽), alkaline phosphatase (ALP) and osteoblast-associated gene core binding factor (Runx-2) in hypoxic PDLSCs were detected by fluorescence quantitative PCR. The expression of Runx-2,Hif-1 偽 and other proteins in PDLSCs cultured with hypoxia was detected by Western blot method. Results the ALP level of PDLSCs in hypoxic group was higher than that in normoxic group, but the ALP level was inhibited at 48 h after hypoxia. The results of fluorescence quantitative PCR and Western blot showed that the osteogenic ability of PDLSCs in hypoxia group was higher than that in normoxic group within 48 h. But hypoxia culture for 48 h inhibited its osteogenic ability. Conclusion hypoxia can significantly enhance the osteogenic ability of PDLSCs during 48 h.
【作者單位】： 安徽醫(yī)科大學(xué)口腔醫(yī)學(xué)院安徽醫(yī)科大學(xué)附屬口腔醫(yī)院安徽省口腔疾病研究中心實驗室;
【基金】：國家自然科學(xué)基金(編號:81271162) 安徽省科技攻關(guān)計劃項目(編號:1401045013)
【分類號】：R781.4

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