【摘要】：目的：本研究是依據(jù)骨基質(zhì)蛋白結(jié)構(gòu)和功能的研究，通過改變氨基酸序列的方式，在RADA16的基礎(chǔ)上合成具有針對成骨細(xì)胞雙重作用的多肽（RADA16-NBD）,并檢測其對炎癥刺激下成骨細(xì)胞遷移、增值能力的影響。為氨基酸多肽的生物學(xué)應(yīng)用以及臨床研究奠定了基礎(chǔ)。 方法：復(fù)蘇成骨細(xì)胞株以及傳代培養(yǎng)；通過混合法制備出RADA16-NBD水凝膠并用掃描電鏡觀察其結(jié)構(gòu)特點(diǎn)；同時(shí)在多肽水凝膠支架RADA16-NBD上接種炎癥刺激下的成骨細(xì)胞；采用劃痕法觀察水凝膠對炎癥刺激下的成骨細(xì)胞遷移能力的影響；通過用CCK-8檢測多肽支架對炎癥刺激下的成骨細(xì)胞的粘附和增值情況。 結(jié)果：通過掃描電鏡可以看出細(xì)胞在水凝膠中得到了良好的伸展；CCK-8的結(jié)果顯示炎癥刺激下的成骨細(xì)胞能夠在RADA16-NBD水凝膠中良好的粘附、和增值；劃痕實(shí)驗(yàn)結(jié)果可看出RADA16-NBD水凝膠對炎癥刺激下成骨細(xì)胞的遷移能力有較高的表達(dá)。 結(jié)論：自組裝多肽水凝膠（RADA16-NBD）能夠支持炎癥刺激下成骨細(xì)胞的粘附、伸展、遷移和增殖，并體現(xiàn)出較高的表達(dá)。顯示其既具有成骨又能抑制炎癥活化的雙重功能，為應(yīng)用于炎癥性骨吸收特別是牙周炎引起的骨缺損的治療開拓一條新的方向和思路。同時(shí)為該實(shí)驗(yàn)的后續(xù)動物實(shí)驗(yàn)奠定了基礎(chǔ)。
[Abstract]:Aim: according to the structure and function of bone matrix protein, RADA16-NBD was synthesized on the basis of RADA16 by changing the amino acid sequence, and the effect of RADA16-NBD on osteoblast migration induced by inflammation was detected. The impact of value-added capabilities. It lays a foundation for the biological application and clinical study of amino acid peptides. Methods: resuscitation osteoblasts were cultured, RADA16-NBD hydrogels were prepared by mixing method and their structural characteristics were observed by scanning electron microscope (SEM). Osteoblasts stimulated by inflammation were inoculated on polypeptide hydrogel scaffolds (RADA16-NBD) at the same time. The effect of hydrogel on the migration ability of osteoblasts stimulated by inflammation was observed by scratch method, and the adhesion and proliferation of osteoblasts stimulated by inflammation were detected by CCK-8. Results: the results of good stretch CCK-8 in hydrogel showed that osteoblasts stimulated by inflammation could adhere to RADA16-NBD hydrogel and increase in value. The results of scratch test showed that RADA16-NBD hydrogel could express the migration ability of osteoblasts stimulated by inflammation. Conclusion: Self-assembled polypeptide hydrogel (RADA16-NBD) can support the adhesion, extension, migration and proliferation of osteoblasts stimulated by inflammation. It is shown that it has the dual function of osteogenesis and inhibition of inflammatory activation, which opens up a new direction and idea for the treatment of inflammatory bone resorption, especially for the treatment of bone defects caused by periodontitis. At the same time, it lays a foundation for the subsequent animal experiment.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R782

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