【摘要】：目的當(dāng)代牙本質(zhì)粘接體系中,無(wú)論是酸蝕-沖洗類(lèi)粘接劑抑或是自酸蝕粘接劑,所形成的混合層底部始終存在一層裸露的膠原纖維。裸露的膠原纖維在牙體內(nèi)源性的膠原酶的攻擊下發(fā)生降解,繼而引發(fā)樹(shù)脂-牙本質(zhì)的粘接強(qiáng)度及粘接持久性下降。本實(shí)驗(yàn)的目的在于探究貽貝粘附蛋白對(duì)膠原酶活性,牙本質(zhì)膠原降解以及牙本質(zhì)粘接微拉伸強(qiáng)度的影響,以期提高牙本質(zhì)粘接的耐久性。方法評(píng)價(jià)貽貝粘附蛋白對(duì)膠原酶活性的影響:本實(shí)驗(yàn)應(yīng)用Sensolyte?MMP通用型分光光度試劑盒檢測(cè)膠原酶活性抑制程度。1mg/ml貽貝粘附蛋白與100U/ml VII型膠原酶(基質(zhì)金屬蛋白酶的代表類(lèi)型之一)為實(shí)驗(yàn)組;GM6001(一種已知的人工合成的基質(zhì)金屬蛋白酶抑制劑)與100U/ml VII型膠原酶為陽(yáng)性對(duì)照組;蒸餾水與100U/ml VII型膠原酶為陰性對(duì)照組。每組分5個(gè)亞組(n=5),充分反應(yīng)后加入檢測(cè)基底液,于412nm波長(zhǎng)下進(jìn)行分光光度檢測(cè),比較酶活性的抑制程度。評(píng)價(jià)貽貝粘附蛋白對(duì)牙本質(zhì)膠原酶解的抑制性:將30片人牙本質(zhì)片隨機(jī)分成三組(n=10):貽貝粘附蛋白組,GM6001組,蒸餾水組。各組牙本質(zhì)片脫礦后分別于膠原酶溶液中溫育7天,取上清液檢測(cè)羥脯氨酸含量,評(píng)估膠原降解程度。評(píng)價(jià)貽貝粘附蛋白對(duì)樹(shù)脂-牙本質(zhì)粘接強(qiáng)度的影響:取60個(gè)新鮮離體牙,暴露冠部牙本質(zhì)后,采用酸蝕-沖洗類(lèi)粘接劑進(jìn)行樹(shù)脂-牙本質(zhì)粘接。根據(jù)酸蝕沖洗后預(yù)處理方式不同,隨機(jī)分成三組:貽貝粘附蛋白組,GM6001組,蒸餾水組。將粘接完成的試件制備成樹(shù)脂-牙本質(zhì)粘接條,然后根據(jù)是否進(jìn)行冷熱循環(huán)(5°C和55°C,2500個(gè)循環(huán))及酶解反應(yīng)(3周,37°C)再將各組分成2個(gè)亞組,分別進(jìn)行樹(shù)脂-牙本質(zhì)粘接的即刻微拉伸檢測(cè)和老化處理后的微拉伸檢測(cè)。掃描電鏡觀察牙本質(zhì)段的拉伸斷面。另取兩個(gè)完整的未拉斷的樹(shù)脂-牙本質(zhì)粘接試件進(jìn)行粘接界面的掃描電鏡檢測(cè)。結(jié)果貽貝粘附蛋白組,GM6001組,蒸餾水組的膠原酶活性(nmol/min/mg)分別為20.22±0.93,29.11±1.17和31.39±0.52,三組間均具有統(tǒng)計(jì)學(xué)差異(p0.01)。各組牙本質(zhì)膠原降解量(羥脯氨酸釋放量μg/m L)分別為2.70±0.53,4.00±1.19和5.40±1.00,三組間均具有統(tǒng)計(jì)學(xué)差異(p0.01)。三組即刻微拉伸強(qiáng)度結(jié)果無(wú)顯著差異,但冷熱循環(huán)及酶解實(shí)驗(yàn)后,三組微拉伸結(jié)果(MPa)呈現(xiàn)統(tǒng)計(jì)學(xué)差異,分別為11.39±2.52,10.77±3.16和5.83±2.02(p0.001)。掃描電鏡檢測(cè)結(jié)果與上述結(jié)果指標(biāo)相一致。即刻粘接界面掃描結(jié)果顯示:貽貝粘附蛋白組的混合層中罕見(jiàn)裸露的膠原纖維,而GM6001組以及蒸餾水組可見(jiàn)少量膠原纖維。酶解反應(yīng)后的粘接界面掃描結(jié)果顯示:貽貝粘附蛋白組以及GM6001組暴露的膠原纖維較蒸餾水組少,且蒸餾水組中的樹(shù)脂突牙本質(zhì)小管中存在較大的間隙。各組微拉伸后的斷面掃描電鏡檢測(cè)結(jié)果顯示:貽貝粘附蛋白組與GM6001組的拉伸斷面大多位于混合層頂端,牙本質(zhì)小管被樹(shù)脂突阻塞,而蒸餾水組的斷面多位于混合層基底部,大量牙本質(zhì)小管呈現(xiàn)空虛狀態(tài)。結(jié)論貽貝粘附蛋白能夠抑制膠原酶活性,抑制牙本質(zhì)膠原纖維的降解,有望提高樹(shù)脂-牙本質(zhì)粘接的耐久性。
[Abstract]:Objective A layer of exposed collagen fibers always exists at the bottom of the mixed layer in the contemporary dentin bonding system, whether it is etch-rinse adhesive or self-etch adhesive. The purpose of this study was to investigate the effects of mussel adhesion protein on the activity of collagenase, the degradation of dentin collagen and the micro-tensile strength of dentin adhesion, so as to improve the durability of dentin adhesion. Inhibition of collagenase activity was detected by kit test. 1 mg/ml Mussel Adhesion Protein and 100U/ml type VII collagenase (one of the representative types of matrix metalloproteinase) were used as experimental group; GM6001 (a known synthetic inhibitor of matrix metalloproteinase) and 100U/ml type VII collagenase were used as positive control group; distilled water and 100U/ml type VII collagenase were used as distilled water and 100U/ml type VII collagenase were used as positive control group. Each group was divided into five subgroups (n=5). After full reaction, the basal fluid was added and the inhibition degree of enzyme activity was measured by spectrophotometry at 412 nm. To evaluate the inhibitory effect of mussel adhesion protein on dentin collagenase hydrolysis, 30 pieces of human dentin tablets were randomly divided into three groups (n=10): mussel adhesion protein group, GM6001 group, distillation. After demineralization, the dentin tablets were incubated in collagenase solution for 7 days. The content of hydroxyproline in the supernatant was measured to evaluate the degree of collagen degradation. According to the different pretreatment methods after etching and rinsing, they were randomly divided into three groups: mussel adhesive protein group, GM6001 group and distilled water group.The bonded specimens were prepared into resin-dentin bonding strips, and then divided into two subgroups according to whether the bonded specimens were subjected to cold and heat cycles (5 C and 55 C, 2500 cycles) and enzymatic hydrolysis (3 weeks, 37 C). The tensile section of dentin segment was observed by scanning electron microscopy. Two intact and unbroken resin-dentin bonding specimens were examined by scanning electron microscopy. Results The collagen of mussel adhesive protein group, GM6001 group and distilled water group were detected by scanning electron microscopy. The activity of enzymes (nmol / min / mg) was 20.22 (+ 0.93), 29.11 (+ 1.17) and 31.39 (+ 0.52), respectively. there were statistical differences among the three groups (p0.01). the degradation of dentin collagen (hydroxyproline release amount (ug / ml) was 2.70 (+ 0.53), 4.00 (+ 1.19) and 5.40 (+ 1.00), respectively. There was no significant difference among the three groups (p0.01). The results of scanning electron microscopy (SEM) were consistent with the above results. Immediate bonding interface scan showed that collagen fibers rarely exposed in the mixed layer of mussel adhesive protein group. A small amount of collagen fibers were found in GM6001 group and distilled water group. Scanning results showed that collagen fibers were less exposed in mussel adhesin group and GM6001 group than in distilled water group, and there were larger gaps in resin-processed dentin tubules in distilled water group. The results showed that most of the tensile sections of the mussel adhesin group and GM6001 group were located at the top of the mixed layer, and the dentinal tubules were obstructed by resin process, while those of the distilled water group were mostly located at the base of the mixed layer, and a large number of dentinal tubules were empty. It is expected that the durability of resin dentin bonding will be improved.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R783.1

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