【摘要】：目的當代牙本質(zhì)粘接體系中,無論是酸蝕-沖洗類粘接劑抑或是自酸蝕粘接劑,所形成的混合層底部始終存在一層裸露的膠原纖維。裸露的膠原纖維在牙體內(nèi)源性的膠原酶的攻擊下發(fā)生降解,繼而引發(fā)樹脂-牙本質(zhì)的粘接強度及粘接持久性下降。本實驗的目的在于探究貽貝粘附蛋白對膠原酶活性,牙本質(zhì)膠原降解以及牙本質(zhì)粘接微拉伸強度的影響,以期提高牙本質(zhì)粘接的耐久性。方法評價貽貝粘附蛋白對膠原酶活性的影響:本實驗應用Sensolyte?MMP通用型分光光度試劑盒檢測膠原酶活性抑制程度。1mg/ml貽貝粘附蛋白與100U/ml VII型膠原酶(基質(zhì)金屬蛋白酶的代表類型之一)為實驗組;GM6001(一種已知的人工合成的基質(zhì)金屬蛋白酶抑制劑)與100U/ml VII型膠原酶為陽性對照組;蒸餾水與100U/ml VII型膠原酶為陰性對照組。每組分5個亞組(n=5),充分反應后加入檢測基底液,于412nm波長下進行分光光度檢測,比較酶活性的抑制程度。評價貽貝粘附蛋白對牙本質(zhì)膠原酶解的抑制性:將30片人牙本質(zhì)片隨機分成三組(n=10):貽貝粘附蛋白組,GM6001組,蒸餾水組。各組牙本質(zhì)片脫礦后分別于膠原酶溶液中溫育7天,取上清液檢測羥脯氨酸含量,評估膠原降解程度。評價貽貝粘附蛋白對樹脂-牙本質(zhì)粘接強度的影響:取60個新鮮離體牙,暴露冠部牙本質(zhì)后,采用酸蝕-沖洗類粘接劑進行樹脂-牙本質(zhì)粘接。根據(jù)酸蝕沖洗后預處理方式不同,隨機分成三組:貽貝粘附蛋白組,GM6001組,蒸餾水組。將粘接完成的試件制備成樹脂-牙本質(zhì)粘接條,然后根據(jù)是否進行冷熱循環(huán)(5°C和55°C,2500個循環(huán))及酶解反應(3周,37°C)再將各組分成2個亞組,分別進行樹脂-牙本質(zhì)粘接的即刻微拉伸檢測和老化處理后的微拉伸檢測。掃描電鏡觀察牙本質(zhì)段的拉伸斷面。另取兩個完整的未拉斷的樹脂-牙本質(zhì)粘接試件進行粘接界面的掃描電鏡檢測。結果貽貝粘附蛋白組,GM6001組,蒸餾水組的膠原酶活性(nmol/min/mg)分別為20.22±0.93,29.11±1.17和31.39±0.52,三組間均具有統(tǒng)計學差異(p0.01)。各組牙本質(zhì)膠原降解量(羥脯氨酸釋放量μg/m L)分別為2.70±0.53,4.00±1.19和5.40±1.00,三組間均具有統(tǒng)計學差異(p0.01)。三組即刻微拉伸強度結果無顯著差異,但冷熱循環(huán)及酶解實驗后,三組微拉伸結果(MPa)呈現(xiàn)統(tǒng)計學差異,分別為11.39±2.52,10.77±3.16和5.83±2.02(p0.001)。掃描電鏡檢測結果與上述結果指標相一致。即刻粘接界面掃描結果顯示:貽貝粘附蛋白組的混合層中罕見裸露的膠原纖維,而GM6001組以及蒸餾水組可見少量膠原纖維。酶解反應后的粘接界面掃描結果顯示:貽貝粘附蛋白組以及GM6001組暴露的膠原纖維較蒸餾水組少,且蒸餾水組中的樹脂突牙本質(zhì)小管中存在較大的間隙。各組微拉伸后的斷面掃描電鏡檢測結果顯示:貽貝粘附蛋白組與GM6001組的拉伸斷面大多位于混合層頂端,牙本質(zhì)小管被樹脂突阻塞,而蒸餾水組的斷面多位于混合層基底部,大量牙本質(zhì)小管呈現(xiàn)空虛狀態(tài)。結論貽貝粘附蛋白能夠抑制膠原酶活性,抑制牙本質(zhì)膠原纖維的降解,有望提高樹脂-牙本質(zhì)粘接的耐久性。
[Abstract]:Objective A layer of exposed collagen fibers always exists at the bottom of the mixed layer in the contemporary dentin bonding system, whether it is etch-rinse adhesive or self-etch adhesive. The purpose of this study was to investigate the effects of mussel adhesion protein on the activity of collagenase, the degradation of dentin collagen and the micro-tensile strength of dentin adhesion, so as to improve the durability of dentin adhesion. Inhibition of collagenase activity was detected by kit test. 1 mg/ml Mussel Adhesion Protein and 100U/ml type VII collagenase (one of the representative types of matrix metalloproteinase) were used as experimental group; GM6001 (a known synthetic inhibitor of matrix metalloproteinase) and 100U/ml type VII collagenase were used as positive control group; distilled water and 100U/ml type VII collagenase were used as distilled water and 100U/ml type VII collagenase were used as positive control group. Each group was divided into five subgroups (n=5). After full reaction, the basal fluid was added and the inhibition degree of enzyme activity was measured by spectrophotometry at 412 nm. To evaluate the inhibitory effect of mussel adhesion protein on dentin collagenase hydrolysis, 30 pieces of human dentin tablets were randomly divided into three groups (n=10): mussel adhesion protein group, GM6001 group, distillation. After demineralization, the dentin tablets were incubated in collagenase solution for 7 days. The content of hydroxyproline in the supernatant was measured to evaluate the degree of collagen degradation. According to the different pretreatment methods after etching and rinsing, they were randomly divided into three groups: mussel adhesive protein group, GM6001 group and distilled water group.The bonded specimens were prepared into resin-dentin bonding strips, and then divided into two subgroups according to whether the bonded specimens were subjected to cold and heat cycles (5 C and 55 C, 2500 cycles) and enzymatic hydrolysis (3 weeks, 37 C). The tensile section of dentin segment was observed by scanning electron microscopy. Two intact and unbroken resin-dentin bonding specimens were examined by scanning electron microscopy. Results The collagen of mussel adhesive protein group, GM6001 group and distilled water group were detected by scanning electron microscopy. The activity of enzymes (nmol / min / mg) was 20.22 (+ 0.93), 29.11 (+ 1.17) and 31.39 (+ 0.52), respectively. there were statistical differences among the three groups (p0.01). the degradation of dentin collagen (hydroxyproline release amount (ug / ml) was 2.70 (+ 0.53), 4.00 (+ 1.19) and 5.40 (+ 1.00), respectively. There was no significant difference among the three groups (p0.01). The results of scanning electron microscopy (SEM) were consistent with the above results. Immediate bonding interface scan showed that collagen fibers rarely exposed in the mixed layer of mussel adhesive protein group. A small amount of collagen fibers were found in GM6001 group and distilled water group. Scanning results showed that collagen fibers were less exposed in mussel adhesin group and GM6001 group than in distilled water group, and there were larger gaps in resin-processed dentin tubules in distilled water group. The results showed that most of the tensile sections of the mussel adhesin group and GM6001 group were located at the top of the mixed layer, and the dentinal tubules were obstructed by resin process, while those of the distilled water group were mostly located at the base of the mixed layer, and a large number of dentinal tubules were empty. It is expected that the durability of resin dentin bonding will be improved.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R783.1

【相似文獻】

相關期刊論文 前10條

1 黎瑞;葉瑋;馮希平;;牙本質(zhì)敏感的概述[J];口腔材料器械雜志;2010年03期

2 陸先韞;牙本質(zhì)和牙髓疼痛的機理[J];國外醫(yī)學參考資料.口腔醫(yī)學分冊;1977年02期

3 陳新民;牙本質(zhì)的力學性質(zhì)[J];國外醫(yī)學.口腔醫(yī)學分冊;1989年01期

4 李富明;牙本質(zhì)小管液滲出狀況的觀察[J];國外醫(yī)學.口腔醫(yī)學分冊;1995年03期

5 李群,施英,趙金濤,張鵬翔;粘結劑滲入牙本質(zhì)深度的喇曼光譜研究[J];實用口腔醫(yī)學雜志;2000年05期

6 梁自民,繆勇建,羅虹,鄧桂祥,曹陽,馬梅;鈣化牙本質(zhì)中神經(jīng)分布的研究[J];廣西醫(yī)科大學學報;2001年01期

7 陳怡,楊貴平,曾泰然;用分光光度法測定人牙本質(zhì)鈣和磷含量[J];貴陽醫(yī)學院學報;2001年01期

8 夏敏,曾紅雨,岳亞莉;髓腔內(nèi)使用牙本質(zhì)小管阻塞劑封閉牙本質(zhì)小管的實驗研究[J];廣東牙病防治;2003年04期

9 區(qū)翠明;牙髓-牙本質(zhì)復合體中第三期牙本質(zhì)的生物學研究[J];醫(yī)學文選;2004年04期

10 陳青宇,袁桂梅,趙越;極固寧治療牙本質(zhì)敏感的臨床療效觀察[J];包頭醫(yī)學院學報;2005年02期

相關會議論文 前10條

1 毛霜霜;張東升;;采用微觀數(shù)字圖像想法測量牙本質(zhì)的失水應變[A];第十二屆全國實驗力學學術會議論文摘要集[C];2009年

2 林瓊光;張成飛;;脈沖Nd:YAG激光照射對牙本質(zhì)通透性的影響[A];全國第四次牙體牙髓病學術會議論文匯編[C];1995年

3 汪竹平;田濟溪;;牙本質(zhì)的神經(jīng)[A];全國第四次牙體牙髓病學術會議論文匯編[C];1995年

4 田力麗;史穎;郭璇;蔡強;崔福齋;;介孔材料負載鈣磷酸鹽治療牙本質(zhì)敏感[A];中華口腔醫(yī)學會口腔材料學專業(yè)委員會第七次全國口腔材料學術交流會論文匯編[C];2011年

5 李全利;劉巍;周潤芝;吳曉婷;許強建;曹穎;梅蕾;朱振雄;;牙本質(zhì)仿生礦化與微結構再生的研究[A];第八屆全國口腔材料學術交流會暨中華口腔醫(yī)學會口腔材料專業(yè)委員會2013年會論文集[C];2013年

6 王彬娉;楊賓;吳卓駿;王秀麗;陳奕苗;;脈沖Nd:YAG激光聯(lián)合3M粘結劑對牙本質(zhì)小管封閉作用的體外研究[A];第八屆全國口腔材料學術交流會暨中華口腔醫(yī)學會口腔材料專業(yè)委員會2013年會論文集[C];2013年

7 陳亞明;嵇穎辰;夏露;孫桂芳;;氟離子導入對牙本質(zhì)小管的封閉作用[A];中華口腔醫(yī)學會口腔材料學專業(yè)委員會第七次全國口腔材料學術交流會論文匯編[C];2011年

8 孫衛(wèi)斌;袁留平;俞未一;丁玉蘭;;草酸鉀對牙本質(zhì)小管堵塞作用的實驗研究[A];第八次全國電子顯微學會議論文摘要集（Ⅰ）[C];1994年

9 李秋瑞;季凌飛;蔣毅堅;;激光輻照牙本質(zhì)小管形成錐體結構的工藝研究[A];中國光學學會2011年學術大會摘要集[C];2011年

10 谷海晶;凌均h，

本文編號：2181610