【摘要】：目的:研究微溝槽鈦片表面經(jīng)三氟代乙烷磺酰氯激活并修飾纖維粘結(jié)蛋白(fibronectin,FN)后對人牙齦成纖維細(xì)胞(HGF)活性的影響。方法:將FN修飾于經(jīng)過激活的微溝槽鈦表面,XPS分析材料表面元素組成;使用cck8法檢測HGF接種材料表面6h、12h、1d、3d、5d、7d后的細(xì)胞增值率;熒光染色觀察接種3d時的細(xì)胞數(shù)量及形態(tài)。I型膠原纖維ELISA實驗及Real-time PCR檢測接種5d時材料表面對HGF分泌功能的差異。結(jié)果:在實驗觀察的6個時間段實驗組材料細(xì)胞增值率高于對照組(P0.05)。3d時HGF在實驗組材料表面的伸展率高于空白組(P0.05)。5d時FN修飾的兩組材料上的細(xì)胞外I性膠原纖維的數(shù)量及mRNA的表達(dá)水平較空白組高(P0.05)。結(jié)論:經(jīng)三氟代乙烷磺酰氯處理修飾FN的微溝槽鈦片可促進(jìn)HGF的增殖、伸展及分泌功能。
[Abstract]:Aim: to study the effect of activated and modified fibronectin FN on the (HGF) activity of human gingival fibroblasts on the surface of microgrooved titanium tablets by trifluoroethanesulfonyl chloride. Methods: FN was modified on the activated microgrooved titanium surface to analyze the surface element composition, and the cell proliferation rate of the HGF inoculated material was detected by cck8 method after 6 hours, 12 hours, 1 day, 3 days and 5 days after inoculation. The number and morphology of type I collagen fibers at 3 days after inoculation were observed by fluorescence staining, and the difference of HGF secretion on the surface of the material was detected by Real-time PCR after 5 days inoculation. Results: the proliferation rate of HGF on the material surface of the experimental group was higher than that of the control group (P0.05). 3 days after the experiment, the extension rate of HGF on the material surface of the experimental group was higher than that of the control group (P0.05). 5 days after FN modification, the extracellular collagen fibers were higher in the experimental group than in the control group (P0.05). The quantity of mRNA and the expression level of mRNA were higher than those of the blank group (P0.05). Conclusion: the microgrooved titanium tablets modified with trifluoroethanesulfonyl chloride can promote the proliferation, extension and secretion of HGF.
【作者單位】： 泉州醫(yī)學(xué)高等專科學(xué)校;莆田學(xué)院附屬醫(yī)院口腔科;福建醫(yī)科大學(xué)附屬口腔醫(yī)院;
【基金】：福建省自然科學(xué)基金項目(編號:2013J05112) 福建省臨床重點�？瀑Y助項目(編號:20121589)
【分類號】：R78

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