【摘要】：目的對比研究兩種人牙源性多能干細(xì)胞重編程前后微小RNAs(mi RNAs)差異表達(dá),交集分析、篩選特異性mi RNAs。方法利用仙臺病毒將人牙髓干細(xì)胞(DPSCs)和根尖乳頭干細(xì)胞(SCAP)重編程為誘導(dǎo)性多潛能干細(xì)胞(i PSCs),提取總RNA,mi RNAs標(biāo)記、雜交,掃描芯片、讀取圖像,篩選差異表達(dá)mi RNAs,交集分析。結(jié)果人DPSCs和SCAP均可重編程為i PSCs。mi RNAs芯片分析結(jié)果顯示人DPSCs重編程后有68個差異表達(dá)mi RNAs(倍數(shù)10),其中37個表達(dá)上調(diào),31個表達(dá)下調(diào);人SCAP重編程后有107個差異表達(dá)mi RNAs(倍數(shù)10),其中68個表達(dá)上調(diào),39個表達(dá)下調(diào)。二者取交集,均上調(diào)的有mi R-302e,下調(diào)的有mi R-29b-3p、mi R-181b-5p、mi R-4328、mi R-22-5p、mi R-145-5p、mi R-4324、let-7b-5p、mi R-181a-5p、mi R-27b-3p(倍數(shù)10)。結(jié)論人DPSCs和SCAP重編程為i PSCs過程中有多種mi RNAs參與,多數(shù)與細(xì)胞周期、上皮-間充質(zhì)轉(zhuǎn)化、轉(zhuǎn)化生長因子β信號通路等相關(guān)。
[Abstract]:Objective to compare the difference of minute RNAs (mi RNAs) expression between two kinds of human odontogenic pluripotent stem cells before and after reprogramming. Methods Human dental pulp stem cell (DPSCs) and apical papillary stem cell (SCAP) were reprogrammed to induce multipotential stem cell (i PSCs),) by Sendai virus to extract total RNAi RNAs labeling, hybridization, scanning chip, reading image, screening differential expression of mi RNAsand intersecting analysis. Results both human DPSCs and SCAP could be reprogrammed as I PSCs.mi RNAs chip analysis results showed that there were 68 differentially expressed mi RNAs (multiples in human DPSCs after reprogramming, 37 of which were up-regulated and 31 were down-regulated. There were 107 differentially expressed mi RNAs (multiples 10 after reprogramming in human SCAP, of which 68 were up-regulated and 39 down-regulated. There was an up-regulation of mi R-302e, and a down-regulation of mi R-29b-3pN mi R-181b-5pan mi R-4328mi R-22-5pN mi R-145-5pN mi R-4324t7b-5pmmi R-181a-5pmi R-27b-3p (multiple 10). Conclusion there are many kinds of mi RNAs involved in the reprogramming of human DPSCs and SCAP into I PSCs, most of them are related to cell cycle, epithelial-mesenchymal transformation, transforming growth factor 尾 signaling pathway and so on.
【作者單位】： 云南省第一人民醫(yī)院口腔內(nèi)科;昆明醫(yī)科大學(xué)第一附屬醫(yī)院心內(nèi)科;
【基金】：國家自然科學(xué)基金(81360161) 云南省教育廳基金(2-015Y153)~~
【分類號】：R781

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