【摘要】：目的探究白介素(IL)-18在顳下頜關(guān)節(jié)炎中的作用。方法 2015年11月—2016年4月,30只SPF級(jí)雄性BALB/c小鼠適應(yīng)性喂養(yǎng)1周后,采用隨機(jī)數(shù)字表法分為A、B、C、D、E組,每組6只。B組~E組在第1、21天時(shí)分別注射Ⅱ型膠原酶;從第1天開始,A組僅注射0.9%氯化鈉溶液,B組注射0.9%氯化鈉溶液,C組注射IL-18,D組注射IL-18結(jié)合蛋白(IL-18BP),E組注射IL-18、IL-18BP。第25天,采集各組小鼠外周血、組織灌洗液,采用流式細(xì)胞術(shù)檢測(cè)外周血單核細(xì)胞/顳下頜關(guān)節(jié)組織灌洗液巨噬細(xì)胞中白介素18受體(IL-18R)陽(yáng)性表達(dá)率,ELISA法檢測(cè)外周血血漿/顳下頜關(guān)節(jié)組織灌洗液上清液中腫瘤壞死因子(TNF)-α、IL-1β水平。結(jié)果 B組、C組小鼠外周血單核細(xì)胞中IL-18R陽(yáng)性表達(dá)率大于A組(P0.05);C組小鼠外周血單核細(xì)胞中IL-18R陽(yáng)性表達(dá)率大于B組、D組、E組(P0.05)。B組~E組小鼠顳下頜關(guān)節(jié)組織灌洗液巨噬細(xì)胞中IL-18R陽(yáng)性表達(dá)率大于A組(P0.05);C組小鼠顳下頜關(guān)節(jié)組織灌洗液巨噬細(xì)胞中IL-18R陽(yáng)性表達(dá)率大于B組、D組、E組(P0.05)。B組~E組小鼠外周血血漿中TNF-α、IL-1β水平高于A組(P0.05);C組小鼠外周血血漿中TNF-α、IL-1β水平高于B組、D組、E組(P0.05)。B組~E組小鼠顳下頜關(guān)節(jié)組織灌洗液上清液中TNF-α、IL-1β水平高于A組(P0.05);C組小鼠顳下頜關(guān)節(jié)組織灌洗液上清液中TNF-α、IL-1β水平高于B組、D組、E組(P0.05);D組小鼠顳下頜關(guān)節(jié)組織灌洗液上清液中TNF-α水平低于B組(P0.05);E組小鼠顳下頜關(guān)節(jié)組織灌洗液上清液中TNF-α水平高于D組(P0.05)。結(jié)論 IL-18能通過(guò)其受體IL-18R刺激單核細(xì)胞/巨噬細(xì)胞產(chǎn)生TNF-α與IL-1β等炎性因子,從而參與顳下頜關(guān)節(jié)炎的發(fā)生發(fā)展,推測(cè)IL-18R可能成為顳下頜關(guān)節(jié)炎的治療靶點(diǎn)。
[Abstract]:Objective to explore the role of interleukins (IL) -18 in temporomandibular arthritis. Methods from November 2015 to April 2016, 30 SPF grade male BALB/c mice were fed for 1 weeks by adaptive feeding, and were randomly divided into A, B, C, D, E group, 6.B groups in each group were injected with type II collagenase at the first 1,21 day. From first days, only 0.9% sodium chloride was injected. Solution, group B was injected 0.9% Sodium Chloride Solution, group C was injected with IL-18, group D was injected with IL-18 binding protein (IL-18BP), group E was injected IL-18, IL-18BP. twenty-fifth days, the peripheral blood of mice was collected and the lavage fluid was collected. Flow cytometry was used to detect the positive expression of interleukin 18 receptor (IL-18R) in macrophages of peripheral blood mononuclear cells / temporomandibular joint Lotion Rate, ELISA method was used to detect the level of tumor necrosis factor (TNF) - alpha and IL-1 beta in the supernatant of peripheral blood plasma / temporomandibular joint tissue lavage fluid. Results the positive rate of IL-18R positive expression in peripheral blood mononuclear cells of group C mice was greater than that of group A (P0.05), and the positive rate of IL-18R positive in peripheral blood mononuclear cells of group C group was greater than that of B group, D group and IL-1 group The positive rate of IL-18R in the macrophages of the TMJ tissue lavage fluid was greater than that of the A group (P0.05), and the positive rate of IL-18R positive expression in the macrophages of the TMJ tissue lavage fluid in the C group was greater than that of the B group, and the TNF- alpha in the peripheral blood plasma of the D group and E group (P0.05).B group was higher than that in the peripheral blood plasma of the group of E (P0.05).B group. The level of TNF- a in the supernatant of the TMJ tissue lavage fluid in group D and group E (P0.05).B was higher than that of group B, and the level of IL-1 beta was higher than that of the A group (P0.05), and the level of TNF- a in the supernatant of the TMJ tissue lavage fluid in the C group was higher than that in the supernatant of the temporomandibular joint. Group (P0.05); the level of TNF- alpha in the supernatant of TMJ tissue in E group was higher than that in group D (P0.05). Conclusion IL-18 can stimulate the development of temporomandibular arthritis by stimulating monocyte / macrophage through its receptor IL-18R to stimulate the development of temporomandibular arthritis. It is presumed that IL-18R may be a therapeutic target for temporomandibular arthritis. Point.
【作者單位】： 錦州醫(yī)科大學(xué);錦州醫(yī)科大學(xué)附屬第一醫(yī)院變態(tài)反應(yīng)與臨床免疫研究中心;錦州醫(yī)科大學(xué)附屬第二醫(yī)院修復(fù)科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81471592)
【分類號(hào)】：R782.6

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