【摘要】：研究目的：通過RNA轉錄組測序技術,檢測口腔鱗癌患者腫瘤組織中與正常組織中基因表達差異,并將差異表達基因與已知的CT抗原文庫進行匹配,篩選出口腔鱗癌組織差異表達的CT抗原基因,進而通過功能聚類分析,了解這些CT抗原在腫瘤發(fā)生發(fā)展中的主要影響,為進一步探尋CT抗原作為口腔鱗癌生物標志物提供實驗和理論依據。研究方法：1.對口腔鱗癌患者腫瘤組織與正常組織的RNA轉錄組測序,獲得初始數據,過濾之后與參考基因組序列進行匹配,得到匹配的有效測序量,根據此結果確定差異基因。2.檢索腫瘤抗原庫,篩選出CT抗原,整理CT抗原抗原基因譜,進而將差異基因與CT抗原基因譜進行比對,得到口腔鱗狀細胞癌差異表達的CT抗原。3.對口腔鱗狀細胞癌差異表達的CT抗原進行功能聚類分析,了解這些CT抗原在腫瘤的發(fā)生發(fā)展中的主要功能和作用。研究結果：1.送檢的口腔鱗癌組織與正常粘膜組中表達差異基因有808個,其中在腫瘤組中表達量高于正常粘膜組的差異基因有470個,表達量低于正常粘膜組的差異基因有338個。2.808個差異基因中有12個差異CT抗原,分別為MAGE家族(CT1.3,CT1.4, CT1.6, CT1.10, CT1.11)、PRAME (CT130)、ARMC3(CT81)、CAGE1(CT95)、 DMRTA1(CT154)、KIFC2(CT139)、LYPD6B(CT116)和TMEFF2(CT120.2)。3.這些差異CT抗原腫瘤的發(fā)生發(fā)展過程中,可能通過以下幾個方面對該進程產生了影響：1、通過與特定蛋白質結合,影響了細胞正常的信號轉導功能,進而對細胞的生長發(fā)育產生影響；2、影響細胞的分化過程,抑制細胞凋亡并促進細胞的增殖；3、與細胞微管結構相結合,并能結合ATP,對細胞分裂過程中染色體的分離產生影響；4、作為跨膜蛋白或者膜錨定蛋白,其脫落可能對腫瘤細胞轉移侵襲產生影響。研究結論：1.在送檢的樣本中,有12個CT抗原在口腔鱗癌中表達；2.在口腔鱗癌中表達的CT抗原分別為：MAGE家族(CT1.3, CT1.4, CT1.6, CT1.10, CT1.11)、PRAME (CT130)、ARMC3(CT81)、CAGE1(CT95)、 DMRTA1(CT154)、KIFC2(CT139)、LYPD6B(CT116)、 TMEFF2(CT120.2)。這些CT抗原可能成為口腔鱗癌早期診斷、腫瘤預后、復發(fā)及轉移的檢測指標。
[Abstract]:Objective: to detect the difference of gene expression between oral squamous cell carcinoma (OSCC) and normal tissues by RNA transcriptome sequencing, and to match the differentially expressed genes with the known CT antigen library. The differentially expressed CT antigen genes in oral squamous cell carcinoma (OSCC) were screened, and the main effects of CT antigens on tumor development were analyzed by functional cluster analysis. To further explore CT antigen as a biomarker of oral squamous cell carcinoma to provide experimental and theoretical basis. Research method: 1. The RNA transcriptome of tumor tissue and normal tissue of oral squamous cell carcinoma were sequenced, the initial data were obtained, and then matched with the reference genomic sequence, the effective sequencing quantity was obtained. According to the results, the differentially expressed gene. 2. The tumor antigen library was searched, CT antigens were screened, CT antigenic gene profiles were sorted out, and then the differentially expressed CT antigens. 3 were obtained by comparing the differentially expressed CT antigens with CT antigenic gene profiles of oral squamous cell carcinoma (OSCC). The functional cluster analysis of CT antigens differentially expressed in oral squamous cell carcinoma (SCC) was carried out to understand the main function and role of CT antigens in tumorigenesis and development. The result of the study was: 1. There were 808 differentially expressed genes in oral squamous cell carcinoma (OSCC) and normal mucosa, of which 470 were higher in tumor group than in normal mucosa group. There were 338 differentially expressed genes in the normal mucosa group. There were 12 differentially expressed CT antigens in the 2.808 differentially expressed genes, namely, MAGE family (CT1.3nCT1.4, CT1.6, CT1.10, CT1.11), PRAME (CT130) ARMC3 (CT81) CAGE1 (CT95), DMRTA1 (CT154) KIFC2 (CT139) LYPD6B (CT116) and TMEFF2 (CT120.2) .3. These differential CT antigenic tumors may have an effect on this process in the following ways: 1, by binding to specific proteins, affecting the normal signal transduction function of the cell. Furthermore, it can affect cell growth and development, affect cell differentiation, inhibit cell apoptosis and promote cell proliferation, bind to cell microtubule structure and bind to ATP, and affect chromosome separation during cell division. 4. As a transmembrane protein or a membrane anchoring protein, its exfoliation may have an effect on the metastasis and invasion of tumor cells. Conclusion: 1. Among the samples examined, 12 CT antigens were expressed in oral squamous cell carcinoma (OSCC). The CT antigens expressed in oral squamous cell carcinoma were as follows: mage family (CT1.3, CT1.4, CT1.6, CT1.10, CT1.11), PRAME (CT130) ARMC3 (CT81) CAGE1 (CT95), DMRTA1 (CT154) KIFC2 (CT139) LYPD6B (CT116), TMEFF2 (CT120.2). These CT antigens may be used as early diagnosis, prognosis, recurrence and metastasis of oral squamous cell carcinoma.
【學位授予單位】：南京大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R739.8

【共引文獻】

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