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84消毒液對藻酸鹽印模消毒的抑菌效果及尺寸穩(wěn)定性研究

發(fā)布時間:2018-07-23 16:17
【摘要】:目的:選用不同濃度的84消毒液,,對藻酸鹽印模用不同時間的浸泡消毒,觀察其抑菌效果及尺寸穩(wěn)定性,找出適合臨床運用的藻酸鹽印模的消毒方法。 方法:制取藻酸鹽印模,分別浸泡在配置金黃色葡萄球菌、大腸桿菌、白色念珠菌和枯草桿菌4種菌懸液中,再分別浸泡5組不同濃度有效氯含量的84消毒液,共20個小組,每組50個印模,共1000個,觀察每個組印模的抑菌效果;重新制取印模后分別浸泡5組不同有效氯含量的84消毒液,每個組10個印模,共50個印模,測量浸泡后與浸泡后的尺寸,觀察每個組印模的尺寸變化。 結(jié)果:(1)有效氯濃度8000mg/L浸泡15min組、有效氯濃度2000mg/L浸泡20min組和有效氯濃度800mg/L浸泡20min組3個組對印模表面以上4種細(xì)菌滅菌效果較好,組間滅菌率比較,P>0.05,無統(tǒng)計學(xué)意義;有效氯濃度400mg/L浸泡30min組和有效氯濃度200mg/L浸泡30min組2個組對印模表面以上4種細(xì)菌滅菌效果較差,組間滅菌率比較,P<0.05,有統(tǒng)計學(xué)意義;有效氯濃度400mg/L浸泡30min組和有效氯濃度200mg/L浸泡30min組分別與其他3組滅菌率比較,P<0.05,有統(tǒng)計學(xué)意義。(2)有效氯濃度8000mg/L浸泡15min組、有效氯濃度2000mg/L浸泡20min組和有效氯濃度800mg/L浸泡20min組3個組的變形率在0.26%-0.39%范圍內(nèi),每組浸泡前后的尺寸變化作比較,P>0.05,無統(tǒng)計學(xué)意義;有效氯濃度400mg/L浸泡30min組和有效氯濃度200mg/L浸泡30min組,2個組的變形率大于2.95%,每組浸泡前后的尺寸變化作比較,P<0.05,有統(tǒng)計學(xué)意義。 結(jié)論:(1)84消毒液有效氯濃度≥800mg/L,對藻酸鹽印模表面大腸桿菌、金黃色葡萄球菌、枯草桿菌和白色念珠菌抑菌效果較好。(2)84消毒液浸泡消毒藻酸鹽印!20min時,印模尺寸變化不明顯,可適用于臨床。
[Abstract]:Objective: to study the bacteriostatic effect and dimensional stability of alginate impression with different concentrations of 84 disinfectant solution, and to find out the disinfection method of alginate impression suitable for clinical use. Methods: the alginate impression was made and soaked in the suspensions of Staphylococcus aureus, Escherichia coli, Candida albicans and Bacillus subtilis respectively. The bacteriostasis effect of each group was observed, and five groups of 84 disinfectant solution with different available chlorine content were soaked separately after remaking. 10 impressions in each group, 50 impressions in each group, were measured after immersion and immersion. Observe the size changes of each group of impressions. Results: (1) 15min group with available chlorine concentration, 20min group with available chlorine concentration 2000mg/L immersion group and 20min group with available chlorine concentration 800mg/L immersion group had better sterilizing effect on 4 kinds of bacteria above the impression surface, and the sterilizing rate between groups was higher than 0.05 (P > 0.05). The sterilizing effect of 30min group with available chlorine concentration 400mg/L and 30min group with available chlorine concentration 200mg/L immersion was poor, and the sterilizing rate was significantly higher than that of 30min group (P < 0.05). The sterilization rates of 30min group and 200mg/L immersion 30min group were significantly higher than those of other three groups (P < 0.05). (2) the effective chlorine concentration of 8000mg/L immersion in 15min group was significantly higher than that in the other three groups (P < 0.05). The deformation rate of 20min group and 20min group were 0.26% -0.39%, and the size changes of each group before and after immersion were compared (P > 0.05), there was no significant difference between the three groups (P > 0.05). The deformation rate of the two groups was more than 2.95 in the 30min group with available chlorine concentration 400mg/L and the 30min group with the available chlorine concentration 200mg/L immersion. The size change of each group before and after immersion was significantly different (P < 0.05). Conclusion: (1) the effective chlorine concentration of 84 disinfection solution 鈮

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