本文選題：干擾 + LRP5�。� 參考：《浙江大學(xué)》2016年碩士論文

【摘要】：目的通過慢病毒技術(shù)構(gòu)建干擾型LRP5慢病毒載體(shRNA-LRP5),在大顆粒噴砂酸蝕表面處理(SLA)的種植體表面轉(zhuǎn)染骨髓基質(zhì)干細(xì)胞(BMSCs),評估LRP5基因的干擾對SLA種植體表面骨結(jié)合的影響。方法種植體表面進(jìn)行大顆粒噴砂酸蝕表面處理(SLA),紫外線消毒兩小時。構(gòu)建LRP5干擾型慢病毒載體(LV-LRP5),轉(zhuǎn)染骨髓基質(zhì)干細(xì)胞(BMSCs)后檢測轉(zhuǎn)染效果以及相關(guān)成骨指標(biāo)。SLA表面處理種植體分為兩組:LV-LRP5組和空白組。實驗中在LV-LRP5組的種植體周圍注射shRNA病毒液,空白組常規(guī)種植。6周后取含有種植體的組織塊進(jìn)行硬組織切片,HE染色觀察并評估新骨的形成,通過測量種植體-骨接觸率和種植體螺紋內(nèi)的骨密度來評價成骨的效果。結(jié)果
[Abstract]:Objective to construct interference LRP5 lentivirus vector (shRNA-LRP5) by lentivirus technique, and transfect bone marrow stromal cells (BMSCs) on the implant surface of large particle acid etching surface treatment (SLA), and to evaluate the effect of LRP5 gene interference on bone binding on the surface of SLA implant. Methods the surface of implant was treated with large particle sand blast acid etching (SLA), and UV disinfection for 2 hours. LRP5 interference lentivirus vector (LV-LRP5) was constructed and transfected into bone marrow stromal cells (BMSCs). In the experiment, shRNA virus solution was injected around the implants of LV-LRP5 group. In the blank group, the tissue blocks containing implants were taken for HE staining and the formation of new bone was evaluated. The effect of osteogenesis was evaluated by measuring implant-bone contact rate and bone mineral density in implant thread. Result
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R783.6

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