本文選題：SP600125 + Tca8113細(xì)胞��； 參考：《延邊大學(xué)》2017年碩士論文

【摘要】：目的:探討JNK特定抑制劑SP600125對人舌鱗癌Tca8113細(xì)胞的增殖、遷移的影響及其機制。方法:用10、20、40μmol/L的JNK抑制劑SP600125處理人舌鱗癌Tca8113細(xì)胞24、48、72h,選用MTT法,檢測SP600125對細(xì)胞增殖的抑制情況;選用細(xì)胞劃痕實驗檢測SP600125對細(xì)胞遷移能力的影響。用10、20、40μmol/L的JNK抑制劑SP600125處理人舌鱗癌Tca8113細(xì)胞48h,選用免疫熒光實驗,檢測JNK和p-JNK蛋白的定位情況;選用Western blot,檢測經(jīng)SP600125處理后JNK和p-JNK蛋白的表達狀況。結(jié)果:1.MTT結(jié)果顯示:Tca8113細(xì)胞經(jīng)10、20、40μmol/LSP600125分別處理24、48、72h后,與對照組相比,用藥組的Tca8113細(xì)胞明顯抑制增殖(P0.01);呈現(xiàn)出濃度和時間依賴性。2.劃痕實驗結(jié)果顯示:Tca8113細(xì)胞經(jīng)10、20、40μmol/L SP600125分別處理24、48、72h后,與對照組相比,用藥組的細(xì)胞移動距離短于對照組(P0.01)。相同藥物濃度時,用藥時間越長,細(xì)胞移動距離越小;藥物作用時間相同時,藥物濃度越高,細(xì)胞移動距離越小。表明SP600125抑制Tca8113細(xì)胞的移動,呈現(xiàn)出濃度依賴性和時間依賴性。3.細(xì)胞免疫熒光染色結(jié)果顯示:Tca8113細(xì)胞經(jīng)10、20、40μmol/LSP600125處理48h,JNK蛋白主要定位在細(xì)胞質(zhì),用藥組與對照組熒光密度未見明顯變化;p-JNK蛋白主要定位在細(xì)胞質(zhì)和細(xì)胞核內(nèi),用藥組與對照組相比,隨著用藥濃度增加,其熒光密度減低。4.Western Blot結(jié)果顯示:Tca8113細(xì)胞經(jīng)10、20、40μmol/L P600125處理48h后提取蛋白,與對照組相比,經(jīng)SP600125處理的Tca8113細(xì)胞中,p-JNK表達減少,且隨著藥物濃度的增加p-JNK表達越低(P0.01)。表明SP600125抑制p-JNK的表達,呈濃度依賴性。結(jié)論:SP600125明顯抑制Tca8113細(xì)胞的增殖、遷移,SP600125的抗腫瘤機制可能與JNK有關(guān)。
[Abstract]:Objective: To investigate the effect of JNK specific inhibitor SP600125 on the proliferation and migration of Tca8113 cells in human tongue squamous cell carcinoma and its mechanism. Methods: the 24,48,72h of Tca8113 cells in human tongue squamous cell carcinoma was treated with JNK inhibitor SP600125 of 10,20,40 mol/L, and MTT method was used to detect the inhibition of cell proliferation, and the cell scratch test was used to detect the cells of the cells. The effect of migration ability. The Tca8113 cell 48h of human tongue squamous cell carcinoma was treated with the JNK inhibitor SP600125 of 10,20,40 micron mol/L. The localization of JNK and p-JNK protein was detected by immunofluorescence test. Western blot was used to detect the expression of JNK and protein after SP600125 treatment. 00125 after the treatment of 24,48,72h, compared with the control group, the Tca8113 cells in the control group significantly inhibited the proliferation (P0.01), and the results showed that the concentration and time dependent.2. scratch test showed that Tca8113 cells treated 24,48,72h after 10,20,40 mu mol/L SP600125 respectively, compared with the control group, the cell migration distance of the drug group was shorter than that of the control group (P0.01). At the same drug concentration, the longer the drug use time, the smaller the cell moving distance, the higher the drug concentration, the smaller the cell moving distance when the drug action time is the same. It shows that SP600125 inhibits the movement of Tca8113 cells, showing the concentration dependent and time dependent.3. cell immunofluorescence staining results show that Tca8113 cells are 10,20,40 mu mol/LSP600 125 treatment of 48h, JNK protein mainly located in the cytoplasm, the fluorescence density of the drug group and the control group did not change obviously, p-JNK protein was mainly located in the cytoplasm and nucleus. Compared with the control group, the drug group decreased with the increase of drug concentration, the fluorescence density of.4.Western Blot fruit showed that Tca8113 cells were treated by 10,20,40 mu mol/L P600125 48. After H extraction, the expression of p-JNK decreased in the Tca8113 cells treated with SP600125, and the expression of p-JNK decreased with the increase of drug concentration (P0.01). It indicated that SP600125 inhibited p-JNK expression in a concentration dependent manner. Conclusion: SP600125 obviously inhibits the proliferation of Tca8113 cells, migration, SP600125 anti-tumor mechanism may be associated with JNK. Close.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.86

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本文編號：2091930