本文選題：納米氧化鋯 + 等離子噴涂　； 參考：《山東大學(xué)》2016年碩士論文

【摘要】：目的：利用等離子噴涂技術(shù)在純鈦種植體表面制備納米氧化鋯涂層,通過檢測鈦基底-納米氧化鋯涂層的表面特性和動物實驗探討其對骨整合的影響,設(shè)計一種既具有良好的生物學(xué)、機械學(xué)性能,又能達到美觀需求的新型種植體,評價該種植體的臨床應(yīng)用前景,提供相關(guān)理論依據(jù)。方法：利用等離子噴涂技術(shù)在純鈦表面制備納米氧化鋯涂層,將鈦基板制作成兩種形狀即圓盤狀試件和無螺紋柱狀種植體,前者用于檢測表面特性,后者用于動物實驗以探討鈦基體-納米氧化鋯涂層對骨結(jié)合的影響。1、表面特性檢測：掃描電鏡(SEM)觀察鈦基體-納米氧化鋯涂層表面微形貌；手持粗糙度檢測儀檢測粗糙度Ra；接觸角測量儀檢測各組試件表面靜態(tài)人工唾液接觸角以評價材料的潤濕性。2、種植體植入實驗：新西蘭雄性大白兔32只,隨機分組。將實驗組即納米氧化鋯涂層鈦種植體(A組)和對照組即純鈦光滑表面種植體(B組)隨機植入實驗動物左、右兩側(cè)脛骨近骨骺端,每組各一枚種植體,植入后2周、4周、8周、12周采用Micro-CT、X線、雙螺旋CT檢測種植體-骨界面特點,亞甲基藍-酸性品紅染色硬組織切片分別進行光學(xué)顯微鏡和掃描電鏡觀察種植周圍新骨生成情況、頂出實驗檢測種植體的生物力學(xué)穩(wěn)定性。實驗數(shù)據(jù)輸入SPSS19.0軟件進行分析。結(jié)果：1、表面特性檢測：B組表面無微孔結(jié)構(gòu),呈不規(guī)則的劃痕,A組表面有等離子噴涂形成的納米級結(jié)構(gòu),微孔大小一致、分布均勻,孔徑50～80μ m,孔深約30μm；A、B兩組表面粗糙度和靜態(tài)人工唾液接觸角分別為(1.55±0.21)μm和(0.179±0.11) μm.(54.61±1.80).和(74.38±3.61)。,兩組差異具有統(tǒng)計學(xué)差異(P0.05)。2、種植體植入實驗：A、B組種植體-宿主骨間均產(chǎn)生骨整合,但Micro-CT和硬組織切片觀察結(jié)果顯示A組比B組骨形成的質(zhì)和量好且在植入早期有新骨形成；A組4、8和12周的骨一種植體結(jié)合率明顯高于B組(P0.05)；頂出實驗結(jié)果顯示,隨植入時間的延長,A、B兩組植入體的最大旋出扭力均升高,同時間點實驗組較對照組高,差異具有統(tǒng)計學(xué)意義,同一處理組不同愈合時期的種植體-宿主骨的最大旋出扭力有明顯的統(tǒng)計學(xué)差異(P0.01),A組的2、4、8和12周的骨結(jié)合力遠遠高于B組(P0.05)。結(jié)論：以上實驗顯示本實驗小組利用等離子噴涂技術(shù)制備的納米氧化鋯涂層與宿主骨之間可以形成良好的骨結(jié)合,該材料具有良好的生物學(xué)性能,與光滑純鈦種植體相比可以改善種植體表面生物活性,促進骨結(jié)合。
[Abstract]:Objective: to prepare the nano zirconia coating on the surface of pure titanium implant by plasma spraying technology. By detecting the surface characteristics of the titanium substrate - nano zirconia coating and the effect of animal experiments on the bone integration, a new type of implant with good biological, mechanical and aesthetic requirements is designed. The clinical application prospect of this implant provides a theoretical basis. Method: the nano zirconia coating is prepared on the surface of pure titanium by plasma spraying technology. The titanium substrate is made into two shapes, namely, disc and non threaded columnar implants. The former is used to detect the surface properties. The latter is used in animal experiments to explore the titanium matrix nano. The effect of zirconia coating on bone binding.1, surface characteristics detection: scanning electron microscopy (SEM) observation of the surface micro morphology of titanium matrix nano zirconia coating; hand-held roughness measuring instrument to detect the roughness Ra; contact angle measuring instrument to detect the contact angle of static artificial saliva on the surface of each specimen to evaluate the wettability of material.2, implant implantation experiment: 32 New Zealand male white rabbits were randomly divided into two groups. The experimental group, the nano zirconia coated titanium implant (A group) and the control group, the pure titanium smooth surface implants (B group) were randomly implanted into the left and proximal tibia proximal epiphysis of the right side of the experimental animals, each group of one implant. After 2 weeks, 4 weeks, 8 weeks, and 12 weeks, the implant was implanted with Micro-CT, X ray, and double spiral CT. The characteristics of body bone interface, methylene blue acid fuchsin staining hard tissue sections were carried out by optical microscope and scanning electron microscope to observe the new bone formation around the implant. The biomechanical stability of the implant was detected by the top out experiment. The experimental data were analyzed by SPSS19.0 software. Results: 1, surface characteristics detection: no microporous structure on the surface of B group. In the irregular scratches, the surface of the A group has a nano scale structure formed by plasma spraying. The size of the micropores is consistent, the pore size is uniform, the pore size is 50~80 mu m, the hole depth is about 30 m; the surface roughness of the two groups of B and the static artificial saliva contact angles are (1.55 + 0.21) mu m and (0.179 + 0.11) mu m. (54.61 + 1.80). And (74.38 + 3.61). The difference of the two group is statistically significant. P0.05.2, implant implantation experiment: A, the implant host bone of group B produced bone integration, but the results of Micro-CT and hard tissue section showed that the quality and quantity of bone formation in the A group were better than that of the B group, and the new bone formation was formed at the early stage of implantation, and the coincidence rate of bone one implant in A group 4,8 and 12 weeks was significantly higher than that in B group (P0.05); the result of the ejection experiment was obvious. The maximum rotation force of the implants in the A and B two groups increased with the time of implantation, and the experimental group was higher than the control group at the same time point. The difference was statistically significant. The maximum rotation force of the implant host bone in the same treatment group was statistically different (P0.01), and the 2,4,8 and 12 weeks of the A group were far higher than those in the group of 2,4,8. In group B (P0.05). Conclusion: the above experiments show that the nano zirconia coating prepared by plasma spraying technology can form a good bone binding between the host bone and the host bone. The material has good biological properties. Compared with the smooth pure titanium implants, it can improve the bioactivity of the implant surface and promote the bone binding.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R783.1

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