本文選題：微種植釘 + 磨牙壓低　； 參考：《吉林大學(xué)》2014年碩士論文

【摘要】：目的： 使用微種植釘壓低犬磨牙，觀察周期性加力不同時間牙周組織中MMP-2的表達,探討微種植釘周期性加力壓低犬磨牙,犬磨牙經(jīng)長期壓低后牙周組織的改建。 方法： 選取健康成年雄性純種Beagle犬3只，于左側(cè)上頜第二、三前磨牙頰、腭側(cè)根分叉區(qū)各植入一枚微型種植釘，施加150g的力壓低犬牙；每4周加力一次。左側(cè)磨牙為實驗組，隨機分為三組，分別加力12、24、36周，右側(cè)磨牙為空白對照。動物處死后，取上頜第二、三前磨牙及其周圍牙周組織，固定、脫鈣及制作組織學(xué)標(biāo)本，切片后行HE染色及MMP-2免疫組化染色并光鏡下觀察，IPP軟件計算MMP-2的平均光密度值，SPSS17.0軟件包對數(shù)據(jù)進行統(tǒng)計學(xué)分析。 結(jié)果： HE染色光鏡下觀察顯示，實驗組較對照組組織學(xué)變化明顯，，牙周膜寬度變窄，成纖維細胞增殖，隨壓低時間變化不明顯；整個固有牙槽骨表面連續(xù)性破壞，加力12周，少量骨吸收陷窩主要出現(xiàn)在牙槽嵴區(qū)和根尖周區(qū)，加力24、36周，骨吸收陷窩較多，并見新骨沉積，成骨細胞排列在其表面；加力12周，根面牙骨質(zhì)光滑，加力24周，根尖區(qū)牙骨質(zhì)連續(xù)性破壞，出現(xiàn)牙骨質(zhì)吸收陷窩，并有新生牙骨質(zhì)覆蓋，至加力36周，吸收陷窩接近根尖牙本質(zhì)。 免疫組化染色結(jié)果顯示，對照組牙周組織中MMP-2僅有少量表達；實驗組，犬磨牙牙周組織中MMP-2的表達量明顯升高，與對照組有顯著性差異；壓低24周牙周組織中MMP-2的表達量達最高；至壓低36周表達量下降，但與對照組仍有顯著性差異；壓低12、24、36周組間相比差異均無顯著性（P0.05）。 結(jié)論： 1．犬磨牙在長期壓低過程中，牙槽骨改建發(fā)生在整個牙槽窩，以牙槽嵴頂部和根尖周區(qū)改建最為活躍。隨著壓低時間的延長，犬牙根尖吸收有逐漸加重的趨勢。提示犬牙在長期壓低過程中，根尖吸收與壓低時間的長短有一定的相關(guān)性; 2．MMP-2主要表達在成纖維細胞、破骨細胞、成骨細胞以及骨細胞胞漿中，實驗組表達量明顯增加，進一步證實MMP-2參與了犬牙長期壓低過程中牙周組織的改建； 3．牙周組織中MMP-2的表達量在實驗組中明顯增多，而沒有隨著壓低時間的延長進一步升高。證實伴隨微種植釘周期性地施加一定的壓低力，經(jīng)長期壓低的犬磨牙牙周組織呈現(xiàn)一種動態(tài)平衡的骨改建過程。
[Abstract]:Objective:
The expression of MMP-2 in the periodontal tissues at different time was observed by the use of the micro implant nails, and the periodontal stress of the canine molar was lowered and the periodontal tissue of the canine molar was remodeled after a long period of depression.
Method:
3 healthy adult male purebred Beagle dogs were selected on the left maxillary second, third premolar buccal and the palatine lateral root bifurcate area. The force of 150g was applied to the lower canine teeth. The left molar was added every 4 weeks. The left molar was divided into three groups randomly, with a force of 12,24,36 weeks and the right molar as the blank control. After the animals were executed, taken from the death of the animals, the animals were executed, taken after the death of the animals, taken after the death of the animals, taken after the death of the animals. The maxillary second, third premolar and its surrounding periodontal tissue were fixed, decalcified and made tissue specimens. After the slice, HE staining, MMP-2 immunohistochemical staining and light microscopy were performed. The mean light density of MMP-2 was calculated by IPP software. The data were statistically analyzed by SPSS17.0 software package.
Result:
HE staining under light microscope showed that the histological changes of the experimental group were obvious, the width of the periodontal membrane was narrower, the proliferation of fibroblasts was not obvious with the low pressure time. The bone surface of the whole alveolar bone was continuously destroyed, and the bone resorption lacunae appeared in the alveolar ridge area and the periapical area for 12 weeks, and the bone resorption lacunae were added for 24,36 weeks, and the bone resorption lacunae were added. The osteoblasts were arranged on the surface of the osteoblast more, and the osteoblasts were arranged on the surface of the osteoblast for 12 weeks. The root surface of the cementum was smooth, and the cementum was continuously destroyed in the apical area for 24 weeks. The cementum resorption lacunae appeared, and the new cementum was covered to 36 weeks. The absorption of the lacunae was close to the root canine essence.
The results of immunohistochemical staining showed that only a small amount of MMP-2 was expressed in the periodontal tissue of the control group, and the expression of MMP-2 in the periodontal tissue of the experimental group was significantly higher than that in the control group. The expression of MMP-2 in the periodontal tissue was the highest in 24 weeks, and the 36 weeks' expression decreased, but there was a significant difference between the control group and the control group. There was no significant difference in 12,24,36 weeks between groups (P0.05).
Conclusion:
1. the alveolar bone remodeling occurred in the entire alveolar nest during the long period of depression, and the reconstruction of the alveolar ridge was most active in the top of the alveolar ridge and the periapical region. With the prolongation of the pressure, the root apex absorption of canine teeth increased gradually.
2.MMP-2 was mainly expressed in fibroblast, osteoclast, osteoblast and osteocyte cytoplasm, and the expression of the experimental group increased significantly, which further confirmed that MMP-2 was involved in the remodeling of periodontal tissue during the long-term depression of canine teeth.
The expression of MMP-2 in the 3. periodontal tissues was significantly increased in the experimental group, but not with the prolongation of the low pressure time. It was proved that the periodontal tissue of the canine molar with a long-term depression showed a dynamic and balanced bone remodeling process.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R783.5

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