本文選題：聚乳酸-羥基乙酸 + 膠原膜��； 參考：《醫(yī)學(xué)研究生學(xué)報》2017年04期

【摘要】：目的低濃度尼古丁可促進(jìn)新生血管的形成,進(jìn)而促進(jìn)創(chuàng)傷愈合,文中旨在觀察低濃度尼古丁膠原膜緩釋微粒系統(tǒng)對大鼠硬腭黏膜創(chuàng)傷愈合的影響。方法以聚乳酸-羥基乙酸(PLGA)共聚物為載體材料,用復(fù)乳揮發(fā)法(w/o/w)制備低濃度尼古丁緩釋微粒,以膠原膜為支架,制備低濃度尼古丁膠原膜緩釋微粒系統(tǒng)。將Wistar大鼠48只分為實驗組和空白組,每組24只,在硬腭前部造直徑3 mm的圓形創(chuàng)口,用6-0可吸收線分別將低濃度尼古丁膠原膜緩釋微粒系統(tǒng)和空白膠原膜縫合于創(chuàng)口上,觀察1、3、7、10 d大鼠上腭組織創(chuàng)口愈合變化情況,并比較不同時間點愈合差異。結(jié)果電鏡下尼古丁緩釋微粒大體觀呈現(xiàn)球形,大小均勻,球體表面粗糙,平均粒徑(3.0±0.2)μm,其包封率和載藥率分別為50.2%和4.12%。尼古丁緩釋微粒第1天體外釋放量較大,第2天釋放量較小,從第3天尼古丁的釋放量趨于穩(wěn)定,且在一定的范圍內(nèi)波動,約在10 d后急劇下降,在第3-10天尼古丁濃度為10-5-10-4mol/L波動。大體觀術(shù)后3 d時,2組創(chuàng)面愈合差異無統(tǒng)計學(xué)意義(P0.05);術(shù)后7 d時實驗組的創(chuàng)口愈合面積大于空白組(P=0.015,);術(shù)后10 d時,2組創(chuàng)面已基本愈合,差異無統(tǒng)計學(xué)意義(P0.05)。術(shù)后7 d時,實驗組上皮增生較空白組明顯,且可見較多的成纖維細(xì)胞、炎癥細(xì)胞及新生的毛細(xì)血管,上皮釘突較短,黏膜下層較疏松且新生大量的膠原纖維,固有層與骨膜緊密連接,創(chuàng)口基本愈合。空白組可見大量的炎性細(xì)胞,創(chuàng)口未完全愈合;術(shù)后10 d時,2組創(chuàng)口上皮細(xì)胞增殖基本已完全完成,創(chuàng)口愈合。結(jié)論低濃度尼古丁膠原膜緩釋微粒系統(tǒng)可能具有促進(jìn)大鼠硬腭黏膜創(chuàng)傷愈合的作用。
[Abstract]:Objective the purpose of this study was to observe the effect of low concentration of nicotine collagen membrane (PLGA) on the wound healing of the hard palate mucosa of rats. Methods the low concentration nicotine was prepared by the poly lactate glycolic acid (PLGA) copolymer as the carrier material and the complex emulsion volatilization (w/o/w) method. A low concentration of nicotine collagen membrane sustained-release particle system was prepared with collagen membrane as a scaffold. 48 Wistar rats were divided into experimental group and blank group, 24 rats in each group, the circular wound of 3 mm in diameter in the front of the hard palate was made, and the low concentration of nicotine collagen membrane sustained release particle system and blank collagen membrane were sutured on the wound with the 6-0 absorbable line. The changes of the healing of the wound healing of the palate tissue in 1,3,7,10 D rats were observed and the difference of healing at different time points was compared. The results showed that the nicotine sustained-release particles were generally spherical, uniform in size, rough on the surface of the sphere, and the average particle size (3 + 0.2) mu m, and the encapsulation efficiency and drug loading rate were 50.2% and 4.12%. of nicotine sustained-release particles, respectively, out of first celestial bodies. The release volume of the second days was smaller, the release amount of nicotine tended to be stable from third days, and it fluctuated in a certain range, decreased sharply after 10 d, and the nicotine concentration was 10-5-10-4mol/L fluctuation on the 3-10 day. At 3 d after the operation, the difference of wound healing in the 2 groups was not statistically significant (P0.05); the wound healing surface of the experimental group was 7 d after the operation. The product was larger than that of the blank group (P=0.015). At 10 d after operation, the wounds of the 2 groups had been basically healed, and the difference was not statistically significant (P0.05). At 7 d after operation, the epithelial hyperplasia in the experimental group was more obvious than that in the blank group, and more fibroblasts, inflammatory cells and newborn capillaries, the epithelial nailed process was short, the submucosa was loose and a large number of collagen fibers were reborn. There was a close connection between the periosteum and the periosteum. The wound was basically healed. A large number of inflammatory cells were found in the blank group and the wound was not completely healed. At 10 d after the operation, the proliferation of the 2 groups of wound epithelial cells was completely completed and the wound healing was healed. Conclusion the low concentration of nicotine collagen membrane sustained-release particle system may have the effect of promoting the healing of the wound of the hard palate mucosa of the rat.
【作者單位】： 安徽醫(yī)科大學(xué)無錫臨床學(xué)院口腔科;解放軍第一0一醫(yī)院口腔科;南京醫(yī)科大學(xué)附屬口腔醫(yī)院基礎(chǔ)教研室;
【基金】：國家自然科學(xué)基金(81272968) 南京軍區(qū)醫(yī)藥衛(wèi)生科研基金(ZD05)
【分類號】：R781

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳鋼,趙士杰,俞光巖,王會信;膠原膜與骨形成蛋白復(fù)合物的成骨作用研究[J];中國口腔種植學(xué)雜志;2001年01期

2 張學(xué)廣;膠原膜及生物活性玻璃用于頜骨囊腫骨缺損修復(fù)[J];現(xiàn)代口腔醫(yī)學(xué)雜志;2002年05期

3 喻建設(shè);丁喜發(fā);許和平;程衛(wèi)林;;醫(yī)用膠原膜在上頜竇瘺修補(bǔ)術(shù)中的應(yīng)用[J];醫(yī)藥論壇雜志;2010年16期

4 陶長仲;;用膠原膜防止在牙周傷口愈合過程中上皮的根尖向遷移[J];國外醫(yī)學(xué).口腔醫(yī)學(xué)分冊;1988年04期

5 盧曉林;徐青峰;童昕;戴建武;胡勤剛;;膠原膜復(fù)合堿性成纖維細(xì)胞生長因子修復(fù)皮膚缺損的實驗研究[J];口腔醫(yī)學(xué)研究;2014年07期

6 林崇韜,車彥海,孫宏晨,歐陽喈;膠原膜在引導(dǎo)牙周組織再生術(shù)中的應(yīng)用[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2002年04期

7 白彭;葉平;吳潤發(fā);戴勇中;;兩種膠原膜暴露于口腔環(huán)境中降解作用的對照研究[J];中國口腔種植學(xué)雜志;2011年01期

8 劉雙喜;徐淑蘭;鐘星華;周敏;楊爍;;堿性成纖維細(xì)胞生長因子/膠原膜修復(fù)大鼠硬腭軟組織缺損的實驗研究[J];廣東醫(yī)學(xué);2011年11期

9 鄭貴成;梁煥友;吳志玲;唐倩;吳堅;任力;;仿生型改性膠原膜的動物學(xué)實驗研究[J];中華口腔醫(yī)學(xué)研究雜志(電子版);2012年03期

10 黃忠鎖,丁曉紅;膠原膜與羥基磷灰石修復(fù)牙槽骨缺損的遠(yuǎn)期療效觀察[J];上�？谇会t(yī)學(xué);2004年03期

相關(guān)會議論文 前2條

1 李成章;尚姝環(huán);樊明文;;雙抗膠原膜引導(dǎo)牙周組織再生的臨床研究——國產(chǎn)膠原膜加工改良與臨床應(yīng)用[A];中華口腔醫(yī)學(xué)會第二次全國會員代表大會暨第七次全國口腔醫(yī)學(xué)學(xué)術(shù)會議論文匯編[C];2001年

2 王海燕;周曾同;;以膠原膜為支架構(gòu)建組織工程化人口腔黏膜的初步研究[A];2004年上海市口腔醫(yī)學(xué)學(xué)術(shù)年會論文匯編[C];2004年

相關(guān)博士學(xué)位論文 前1條

1 袁繼龍;復(fù)合骨生長刺激因子的膠原膜修復(fù)下頜骨缺損的應(yīng)用研究[D];南方醫(yī)科大學(xué);2007年

相關(guān)碩士學(xué)位論文 前7條

1 董露;富血小板纖維蛋白（PRF）誘導(dǎo)牙齦軟組織修復(fù)再生的臨床觀察[D];四川醫(yī)科大學(xué);2015年

2 李文浩;國產(chǎn)納米級羥基磷灰石復(fù)合膠原膜的組織學(xué)評估[D];四川大學(xué);2004年

3 段莉;生物活性玻璃聯(lián)合膠原膜引導(dǎo)牙周組織再生的實驗研究[D];昆明醫(yī)學(xué)院;2004年

4 劉雙喜;bFGF結(jié)合膠原膜修復(fù)大鼠硬腭軟組織缺損的實驗研究[D];南方醫(yī)科大學(xué);2011年

5 朱卓立;兩種膠原膜修復(fù)骨缺損的動物試驗研究[D];四川大學(xué);2006年

6 余科;國產(chǎn)納米級羥基磷灰石復(fù)合膠原膜（nHAC）降解產(chǎn)物對成骨細(xì)胞生物相容性的實驗研究[D];四川大學(xué);2006年

7 曲豐江;人骨髓間充質(zhì)干細(xì)胞體外成骨分化及與Bio-gide膠原膜復(fù)合培養(yǎng)的實驗研究[D];南昌大學(xué);2007年

，

本文編號：2035850