本文選題：鈦 + 種植體��； 參考：《青島大學(xué)》2014年碩士論文

【摘要】：目的： 本研究通過整理之前完成的實(shí)驗(yàn)及數(shù)據(jù),搜集大量文獻(xiàn),找出較為合適的純鈦種植體弧氧化處理?xiàng)l件(電壓、占空比、電解液配比),對純鈦種植體進(jìn)行表面處理。通過動物實(shí)驗(yàn)研究微弧氧化表面處理的純鈦種植體與單純通過機(jī)械進(jìn)行光滑表面處理的純鈦種植體相比,是否有更好的生物相容性、更好的骨誘導(dǎo)作用、是否能夠達(dá)到更好的骨結(jié)合。以及研究其優(yōu)越性主要在哪些方面體現(xiàn)。為未來微弧氧化處理種植體應(yīng)用于臨床做好理論基礎(chǔ)。 方法： 1.通過不同實(shí)驗(yàn)條件下多組微弧氧化純鈦種植體的掃描電鏡檢查(SEM)、X射線能譜分析(EDS)、表面粗糙度(RA)分析,以及對相關(guān)文獻(xiàn)的查閱分析,配比出合適的電解液、制定合適的物理參數(shù)。 2.將32枚機(jī)械光滑種植體、32枚微弧氧化種植體隨機(jī)植入新西蘭大白兔的股骨。動物處死時(shí)間分別定于2周、4周、8周、12周。處死前13、14天注射鹽酸四環(huán)素。處死前3、4天注射鈣黃綠素。制作含種植體的骨膜片。使用熒光顯微鏡激發(fā)觀察熒光染色情況、再進(jìn)行激光共聚焦的細(xì)部觀察,使用Image-pro plus6.0測量熒光條帶寬度,進(jìn)行計(jì)算。使用SPSS16.0進(jìn)行統(tǒng)計(jì)學(xué)分析。觀察完畢后將切片進(jìn)行酸性品紅-亞甲基藍(lán)染色。觀察種植體-骨界面的骨整合情況,按照時(shí)間、組別進(jìn)行縱向、橫向比對。 結(jié)果： 電解液使用為0.13mol/l乙酸鈣、0.06mol/l磷酸二氫鈉。脈沖電壓300v,頻率為700Hz,占空比為5%,微弧氧化處理時(shí)間為10min,恒定穩(wěn)定為35℃。 1.熒光顯微鏡下觀察不脫鈣種植體骨磨片,與染色病理切片對比,尋找到代謝較為活躍的骨細(xì)胞。對于機(jī)械光滑表面組,骨界面的成骨主要為距離成骨,接觸成骨較少。微弧氧化組除與光滑表面組程度相近的距離成骨外還有著大量活躍的接觸成骨。 2.對種植體磨片進(jìn)行激光共聚焦的觀察,拍攝后可以觀察到種植體側(cè)的兩條熒光帶的寬度不同,使用圖像軟件對寬度進(jìn)行測量,計(jì)算出兩組不同時(shí)間段的礦化速率。機(jī)械光滑組在4、8、12周礦化率分別為1.14±0.13μ m/d、1.38±0.13μ m/d、0.76±0.06μm/d,微弧氧化組在4、8、12周礦化速度分別為1.35±0.10μm/d、1.45±0.13μm/d、0.93±0.07μ m/d,微弧氧化組在三個(gè)時(shí)間段礦化率均大于光滑組,使用SPSS16.0進(jìn)行統(tǒng)計(jì)學(xué)分析后具有統(tǒng)計(jì)學(xué)意義(P=0.00) 3.各期0I值的測定 (1)兩周0I值,微弧氧化組的0I值高于光滑組(P=0.00)。 (2)四周0I值,微弧氧化組的0I值高于光滑組(P=0.00)。 (3)八周0I值,微弧氧化組的0I值高于光滑組(P=0.00)。 (4)十二周0I值,微弧氧化組的0I值高于光滑組(P=0.00) 4.對種植體酸性品紅-亞甲基藍(lán)染色切片的觀察：發(fā)現(xiàn)微弧氧化組在第2、4、8、12周的種植體切片中,其成骨細(xì)胞的數(shù)量、新生骨面積要多于機(jī)械光滑組。微弧氧化組的接觸成骨要高于機(jī)械光滑組,而距離成骨方面,兩組之間無明顯差別。在接近種植體的骨接觸區(qū)微弧氧化組的骨細(xì)胞、哈弗氏系統(tǒng)的數(shù)量要多于光滑表面組。 結(jié)論： 微弧氧化處理組的生物相容性要優(yōu)于光滑對照組。微弧氧化處理種植體的優(yōu)越性主要體現(xiàn)在接觸成骨方面。通過種植體與骨組織接觸區(qū)更多的成骨細(xì)胞數(shù)量以及更快的成骨速率表現(xiàn)出來。
[Abstract]:Purpose :

This study collected a lot of literature through the experiment and data before finishing , and found suitable conditions ( voltage , duty ratio , electrolyte proportion ) of pure titanium implant . The pure titanium implants treated by micro - arc oxidation surface were treated by animal experiments .

Method :

1 . Scanning electron microscopy ( SEM ) , X - ray energy analysis ( EDS ) , surface roughness ( RA ) analysis of multiple groups of micro - arc oxidation pure titanium implants under different experimental conditions , as well as the review and analysis of the relevant literatures , the appropriate electrolyte was prepared , and appropriate physical parameters were developed .

2 . Thirty - two mechanically smooth implants and 32 microarc oxidation implants were randomly implanted into the femur of New Zealand White rabbits . The animals were sacrificed for 2 weeks , 4 weeks , 8 weeks and 12 weeks . The results were as follows : 4 weeks , 4 weeks , 8 weeks , 12 weeks .

Results :

The electrolyte is 0.13mol / l calcium acetate and 0.06 mol / l sodium dihydrogen phosphate . The pulse voltage is 300v , the frequency is 700Hz , the duty ratio is 5 % , the micro - arc oxidation treatment time is 10min , and the constant stability is 35 DEG C .

1 . No calcium - free implant bone was observed under the fluorescence microscope . Compared with the stained pathological sections , it was found that there was a more active bone . For the mechanical smooth surface group , the osteogenesis of the bone interface was mainly the distance from the bone to the bone . The micro - arc oxidation group had a large number of active contact bones except for the distance from the smooth surface group to the bone .

2 . The results showed that the mineralization rates of the two groups were 1.14 鹵 0.13 渭m / d , 1 . 38 鹵 0.13 渭m / d , 0 . 76 鹵 0 . 0渭m / d , 0 . 93 鹵 0 . 07渭m / d , respectively .

3 . Determination of 0I value for each period

( 1 ) The value of 0I in micro - arc oxidation group was higher than that in smooth group ( P = 0.00 ) .

( 2 ) The value of 0I in micro - arc oxidation group was higher than that in smooth group ( P = 0.00 ) .

( 3 ) The value of 0I in micro - arc oxidation group was higher than that in smooth group ( P = 0.00 ) .

( 4 ) The value of 0I in micro - arc oxidation group was higher than that of smooth group ( P = 0.00 ) .

4 . Observations on the staining sections of acid red - methylene blue in implants showed that the number of osteoblasts in the microarc oxidation group was more than that of the mechanical smooth group in the 2nd , 4th , 8th , 12th week implant sections . The contact of the microarc oxidation group was higher than that of the mechanical smoothing group .

Conclusion :

The biocompatibility of the micro - arc oxidation treatment group is superior to that of the smooth control group . The advantages of micro - arc oxidation treatment implant are mainly in contact with the bone .
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R783.6

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相關(guān)期刊論文 前10條

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本文編號：2010166