本文選題：超聲潔治 + 顯微鏡　； 參考：《武漢大學(xué)》2017年碩士論文

【摘要】：目的收集離體牙,分別在顯微鏡下與直視下超聲潔治處理后,觀察離體牙的根面情況,以此作為參考,評(píng)價(jià)牙科顯微鏡在超聲潔治中的作用,為臨床使用顯微鏡提供指導(dǎo)依據(jù)。方法1.選擇因重度牙周炎拔除的患牙10顆,每顆牙通過隨機(jī)數(shù)法分成兩面。一面在顯微鏡下進(jìn)行超聲潔治,另一面在直視下超聲潔治。潔治后用亞甲藍(lán)染色分析,觀察牙根表面結(jié)石殘留情況。2.選擇因重度牙周炎拔除的單根牙6顆及因正畸拔除的單根牙2顆,通過隨機(jī)數(shù)法分成4組,分別為顯微鏡組,直視組,未處理組和正畸牙組。前兩組潔治后,將4組用金剛砂車針制成3mm*3mm*1mm的牙根片,于掃描電鏡下觀察。3.選擇因重度牙周炎拔除的單根牙6顆及因正畸拔除的單根牙2顆,通過隨機(jī)數(shù)法分成4組,分別為顯微鏡組,直視組,未處理組和正畸牙組。前兩組潔治后,截取根柱部位,于納米壓痕儀掃描觀察。4.選擇因重度牙周炎拔除的患牙8顆,使用隨機(jī)數(shù)法分成直視組和顯微鏡組。潔治后制成3mm*3mm*1mm的牙根片,置于96孔板中接種牙周膜細(xì)胞,進(jìn)行CCK-8實(shí)驗(yàn)。結(jié)果直視組和顯微鏡組結(jié)石殘留差異具有顯著性(P0.05),顯微鏡下結(jié)石去除效果較好。電鏡下顯微鏡組表面較平整,有少量碎屑存在,但較直視組少,未見明顯劃痕。納米壓痕儀結(jié)果與電鏡觀察一致。CCK8實(shí)驗(yàn)結(jié)果顯示顯微鏡組和直視組差異具有顯著性(P0.05)。顯微鏡組的od值較直視組高,說明顯微鏡組的細(xì)胞增殖較高,更有利于細(xì)胞附著生長(zhǎng)。結(jié)論顯微鏡有助于提高超聲潔治的治療效果。
[Abstract]:Objective to evaluate the effect of dental microscope on ultrasonic scaling and to provide guidance for clinical application of ultrasonic scaling for the purpose of observing the root surface of isolated teeth after ultrasonic scaling under microscope and under direct vision respectively, and to evaluate the role of dental microscope in ultrasonic scaling. Method 1. Ten teeth with severe periodontitis were selected and each tooth was divided into two sides by random number method. One side under the microscope under the ultrasonic scaling, the other side under the direct view of ultrasonic scaling. After scaling, methylene blue staining was used to observe the residual condition of stone on the root surface. Six single teeth extracted from severe periodontitis and 2 teeth from orthodontic treatment were randomly divided into four groups: microscope group, direct vision group, untreated group and orthodontic tooth group. After the first two groups were cleansed, 4 groups were made into root slices of 3mm*3mm*1mm with Emery needle, and observed under scanning electron microscope. Six single teeth extracted from severe periodontitis and 2 teeth from orthodontic treatment were randomly divided into four groups: microscope group, direct vision group, untreated group and orthodontic tooth group. After the first two groups were treated, the root column was cut off and observed by nano indentation. Eight teeth with severe periodontitis were selected and randomly divided into open vision group and microscope group. The root slices of 3mm*3mm*1mm were prepared after cleaning and were inoculated with periodontal ligament cells in 96 hole plate for CCK-8 experiment. Results there was a significant difference between the two groups in stone residue (P 0.05), and the removal of stones under microscope was better. Under the electron microscope, the surface of the microscope group was flat, a little debris existed, but less than that of the direct vision group, and no obvious scratch was found. The results of nanoindentation were consistent with the observation of electron microscope. The results of CCK8 experiment showed that there was a significant difference between the microscope group and the direct vision group (P 0.05). The OD value of microscope group was higher than that of direct vision group, which indicated that the cell proliferation in microscope group was higher, which was more favorable for cell attachment and growth. Conclusion microscope is helpful to improve the therapeutic effect of ultrasonic scaling.
【學(xué)位授予單位】：武漢大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781.4

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