本文選題：蜂膠 + 菌斑生物膜　； 參考：《新疆醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：研究伊犁黑蜂蜂膠對口腔常見致齲菌及其生物膜毒力因子的作用,探討其防齲效果及可能的防齲機(jī)制。方法：①通過液體稀釋法測定伊犁黑蜂蜂膠對口腔常見致齲菌的最低抑菌濃度(MIC)及最低殺菌濃度(MBC)；②結(jié)晶紫染色法測定伊犁黑蜂蜂膠對口腔常見致齲菌的最小生物膜清除濃度(MBEC);③以MIC以下的4個(gè)濃度(包括MIC濃度)配置成初始pH為7.0的含藥培養(yǎng)基,以空白液體培養(yǎng)基作為對照組,加入菌液厭氧培養(yǎng)后測其pH值,并計(jì)算pH變化值(△pH值)以檢測不同濃度黑蜂蜂膠對浮游狀態(tài)下測試菌產(chǎn)酸能力的影響；④葸酮法測定MIC以下4個(gè)濃度(包括MIC濃度)的黑蜂蜂膠對變形鏈球菌、粘性放線菌產(chǎn)生胞外多糖的影響,空白液體培養(yǎng)基作為對照組；⑤培養(yǎng)測試細(xì)菌24h單菌生物膜,加入MBEC以下的四個(gè)濃度(包括MBEC濃度)配置成初始pH值為7.0的含藥培養(yǎng)基,厭氧培養(yǎng)24h后測pH值,并計(jì)算pH變化值(△pH值)以檢測不同濃度黑蜂蜂膠對測試菌單菌生物膜狀態(tài)下產(chǎn)酸能力的影響；⑥葸酮法測定MBEC以下的四個(gè)濃度(包括MBEC濃度)的黑蜂蜂膠對變形鏈球菌24h單菌生物膜產(chǎn)生水不溶性胞外多糖的影響。結(jié)果：①伊犁黑蜂蜂膠對變形鏈球菌、遠(yuǎn)緣鏈球菌、嗜酸乳桿菌、血鏈球菌、粘性放線菌、內(nèi)氏放線菌的MIC分別是0.781ng·mL-1、0.39mg·mL-1、1.56mg· mL-1、0.39mg·mL-1、0.2mg·ml-1、0.2mg·mL-1; MBC分別為1.56mg·mL-11和0.78mg·mL-1、3.125mg·mL-1、0.78mg·mL-1、0.39mg·mL-1、0.39mg·mL-1;②伊犁黑蜂蜂膠對變形鏈球菌、遠(yuǎn)緣鏈球菌、血鏈球菌、粘性放線菌、內(nèi)氏放線菌的MBEC分別是6.25mg·mL-1、1.56mg·mL-1、3.1256mg·mL-1、0.78mg·mL-1;③蜂膠可使各游離狀態(tài)下的測試菌△pH降低,蜂膠各組與對照組相比差異均有統(tǒng)計(jì)學(xué)意義(P0.05)；④在MIC濃度時(shí),蜂膠可使變形鏈球菌、粘性放線菌合成胞外多糖的能力降低,蜂膠組與對照組相比差異均有統(tǒng)計(jì)學(xué)意義(P0.05)；⑤伊犁黑蜂蜂膠可使各測試菌單菌生物膜△pH降低,蜂膠各組與對照組相比差異均有統(tǒng)計(jì)學(xué)意義(P0.05)；⑥在MBEC濃度時(shí),蜂膠可使變形鏈球菌合成水不溶性胞外多糖的能力降低,蜂膠組與對照組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論：伊犁黑蜂蜂膠對口腔主要致齲細(xì)菌及其菌斑生物膜的生長、產(chǎn)酸、產(chǎn)糖均有不同程度的抑制作用,是具有一定防齲效果的天然藥物。
[Abstract]:Objective: to study the effect of Propolis on dental caries causing bacteria and its biofilm virulence factor, and to explore its anti-caries effect and possible anti-caries mechanism. Methods the minimum inhibitory concentration (MIC) of propolis against common dental caries bacteria and the minimum bactericidal concentration (MBC) were determined by liquid dilution method. The minimal growth of propolis against common dental caries was determined by the method of crystal violet staining. The membrane scavenging concentration (MBEC) 3 was configured with four concentrations below MIC (including MIC concentration) to form a medicine-containing medium with initial pH 7.0. Blank liquid medium was used as control group. The pH value was measured after anaerobic culture of bacteria solution, and pH value (pH value) was calculated to detect the effect of different concentrations of black bee propolis on acid production ability of bacteria in planktonic state. 4 the effects of four concentrations of MIC (including MIC) on Streptococcus mutans and extracellular polysaccharides produced by actinomycetes were determined by anthracanone method. The blank liquid medium was used as the control group to test the biofilm of single bacteria for 24 hours. Four concentrations of MBEC (including MBEC) were added to the medium with initial pH 7.0. After 24 hours of anaerobic culture, pH values were measured. The pH value (pH value) was calculated to detect the effect of different concentrations of Propolis on the acid-producing ability of monobacteria biofilm. 6The effects of four concentrations of MBEC (including MBEC) on the production of water-insoluble extracellular polysaccharides from the biofilm of Streptococcus mutans were determined by anthracanone method. Results the MIC of the propolis against Streptococcus mutans, Streptococcus distalis, Lactobacillus acidophilus, Streptococcus sanguis, Actinomyces glutinis and Actinomyces Neisseria were 0.781ng mL-1 0.39mg mL-1n 1.56mg mL-1n 0.39mg mL-1n 0.2mg ml-10.2mg mL-1mg respectively, and MBC were 1.56mg mL-11 and 0.78mg mL-1.3.125mg mL -1 0.78mg ml ~ (-1) 0.39mg ml ~ (-1) ~ 0.39mg mg ~ (-1) mg 路ml ~ (-1) for Streptococcus mutans, respectively. The MBEC of Streptococcus distalis, Streptococcus sanguis, Actinomyces glutinis and Actinomycetes Neoris were 6.25mg mL-1 1.56mg mL-1and 3.1256mg mL-1 0.78mg mL -1 propolis, respectively. Propolis could reduce the pH of the tested bacteria in each free state. The difference of propolis between each group and the control group was statistically significant when the MIC concentration was higher than that in the control group (P 0.05). Propolis could reduce the ability of Streptococcus mutans and actinomycetes to synthesize extracellular polysaccharides. The propolis group had significant difference from the control group. Propolis could reduce the pH of the biofilm of the tested bacteria. There was significant difference between propolis group and control group (P 0.05). Propolis could reduce the ability of Streptococcus mutans to synthesize insoluble extracellular polysaccharides when the concentration of propolis was higher than that in control group (P 0.05), and the difference between propolis group and control group was statistically significant (P 0.05). Conclusion: Yili bee propolis can inhibit the growth, acid production and sugar production of the main dental caries bacteria and their plaque biofilm in different degrees, and is a natural drug with a certain anti-caries effect.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R781.1

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本文編號：1990653