本文選題：電解蝕刻 + 鈦合金　； 參考：《吉林大學(xué)》2014年碩士論文

【摘要】：目的：種植體的成功標(biāo)準(zhǔn)除了具有良好的骨結(jié)合,還要求形成良好的種植體周軟組織封閉。影響種植體周軟組織封閉的重要決定因素為種植體頸部的表面形貌。本實驗旨在比較通過機(jī)械加工法(M)、直流電解蝕刻法(DC)、交流電解蝕刻法(AC)三種不同的方法處理鈦合金試件得到的三種不同的表面形貌對人牙齦成纖維細(xì)胞的附著的影響，尋找出一種有利于成纖維細(xì)胞生長以及軟組織附著的理想表面，為今后種植體頸部設(shè)計提供指導(dǎo)。 方法：通過機(jī)械加工法（M）、直流電解蝕刻法(DC)、交流電解蝕刻法(AC)成形三種不同鈦合金表面形貌，使用掃描電子顯微鏡觀察其表面微觀形態(tài)，將其與成纖維細(xì)胞共同培養(yǎng)，吖啶橙染色法檢測生物相容性；MTT法檢測表面細(xì)胞的增殖情況；掃描電鏡觀察表面細(xì)胞的形狀及排列。 結(jié)果：通過機(jī)械加工(M)、直流電解蝕刻(DC)、交流電解蝕刻(AC)三種處理方法分別得到了較光滑的表面、微米-亞微米孔表面、微米-亞微米-納米結(jié)構(gòu)表面。通過吖啶橙染色法顯示成纖維細(xì)胞在三種表面形貌的鈦片上均具有良好的細(xì)胞活性，核仁清晰，胞漿飽滿，提示三種表面形貌的鈦片均具有良好生物相容性。MTT結(jié)果顯示，三種表面形貌的鈦片吸光度值變化趨勢基本一致，均呈遞增趨勢。光滑表面組與微米-亞微米表面組和微米-亞微米-納米組表面吸光度值有統(tǒng)計學(xué)差異。在第3-5天，微米-亞微米-納米組表面吸光度值高于微米-亞微米表面，提示成纖維細(xì)胞在微米-亞微米-納米表面的增殖情況最優(yōu)。將成纖維細(xì)胞與三組鈦片共同培養(yǎng)的第3天，掃描電鏡觀察可見，各組鈦件表面胞外基質(zhì)較少，可見明顯未被基質(zhì)覆蓋的溝、凹孔形態(tài)。M組鈦片低倍鏡下可見細(xì)胞扁平，呈圓形，近似平行排列，細(xì)胞長軸與溝紋方向一致，伸展較差，高倍鏡下可見細(xì)胞伸出許多微絨毛，偽足較少且短；DC組低倍鏡下可見細(xì)胞附著于微米孔內(nèi)，伸展較好；高倍鏡下可見細(xì)胞豐滿，細(xì)胞表面微絨毛及突起較少，伸出的偽足較粗較長，錨固于微米孔壁上；AC組低倍鏡下可見細(xì)胞附著于微米孔內(nèi)，形態(tài)更加伸展，高倍鏡下可見細(xì)胞豐滿，細(xì)胞表面有許多突起及微絨毛，伸出的偽足更寬更長，可成帶狀，相鄰細(xì)胞的偽足可發(fā)生融合。將鈦片與成纖維細(xì)胞共同培養(yǎng)的第7天，掃描電鏡觀察可見，各組鈦件表面胞外基質(zhì)較第3天明顯增多。M組低倍鏡下可見材料表面溝紋被細(xì)胞外基質(zhì)大面積覆蓋，細(xì)胞呈圓形，伸展較差；高倍鏡下，細(xì)胞伸出的微絨毛緊密的貼在材料表面，細(xì)胞伸出的偽足較第3天增長增多，相鄰細(xì)胞的偽足可相融合。DC組低倍鏡下可見微米孔被大量細(xì)胞外基質(zhì)覆蓋；高倍鏡下可見細(xì)胞完全展開，呈扁平片狀，少量細(xì)胞呈球形，可能為基質(zhì)或細(xì)胞表面上新分裂的細(xì)胞，細(xì)胞伸出許多偽足和微絨毛，緊緊附著于微米孔的孔壁上。AC組低倍鏡下可見細(xì)胞分泌的基質(zhì)大面積覆蓋在材料表面，高倍鏡下可見細(xì)胞形態(tài)更加扁平不規(guī)則，細(xì)胞胞體位于微米孔內(nèi)，向鄰近微米孔壁伸出大量偽足及絨毛，偽足可相互交織成網(wǎng)狀；細(xì)胞牢固的固定于微米孔中，可見長梭形及球形新生細(xì)胞，有的細(xì)胞伸出細(xì)長的偽足，呈懸空狀，可伸入到相隔較遠(yuǎn)的納米孔內(nèi)部。 結(jié)論：通過交流電解蝕刻法形成的兼具微米-亞微米-納米三級孔結(jié)構(gòu)的鈦合金表面具有良好的生物相容性，能夠促進(jìn)成纖維細(xì)胞的附著，，增殖及細(xì)胞基質(zhì)的分泌。通過該方法處理出的種植體頸部表面是一種較優(yōu)秀的表面，可為以后的相應(yīng)體內(nèi)實驗及種植體表面處理提供指導(dǎo)。
[Abstract]:Objective : To study the effect of three different surface morphologies on the adhesion of the implant neck with three different methods : mechanical machining ( M ) , direct current etching ( DC ) , AC electrolytic etching ( AC ) , and to find an ideal surface for the growth of fibroblasts and the attachment of soft tissues , which will provide guidance for the neck design of implants .

Methods : The surface morphology of three different titanium alloys was formed by mechanical machining ( M ) , direct current etching ( DC ) and alternating current electrolytic etching ( AC ) . Scanning electron microscope ( SEM ) was used to observe its surface morphology , which was co - cultured with fibroblasts , and acridine orange staining was used to detect biocompatibility ;
MTT assay was used to detect the proliferation of surface cells .
Scanning electron microscope was used to observe the shape and arrangement of surface cells .

Results : The results showed that three kinds of surface morphology of fibroblasts were obtained by mechanical processing ( M ) , direct current etching ( DC ) and alternating current electrolytic etching ( AC ) .
In DC group , the visible cells were attached to the micro - pores and extended well .
Under the microscope , the visible cells are plump , the surface microvilli and the protrusion of the cells are less , the extended pseudopod is thicker and longer , and is anchored on the wall of the micron pore ;
Under the low - magnification microscope , the visible cells were attached to the micrometer pores , the morphology was more extended , the cells under the microscope were full , the surface of the cells had a lot of protrusions and microvilli , and the surface of the cells was wider and longer . The outer matrix of the surface of the titanium pieces was obviously increased on the 7th day . The surface of the surface of the titanium pieces was covered with large area of the extracellular matrix , and the cells were round and stretched .
Under the microscope , the microvilli extending from the cells were closely attached to the surface of the material , and the pseudopods of the cells extended more than the third day , and the pseudopodium of adjacent cells could be fused .
Under high magnification , the visible cells are completely expanded , flattened , and small cells are spherical , which may be newly divided cells on the surface of the matrix or cells . The cells extend out of many pseudopods and microvilli and adhere tightly to the pore walls of micron pores . The cells secreted by the cells under the low magnification of the AC group are covered on the surface of the material .
The cells are firmly fixed in the micro - pores , and can be found to have long fusiform and globular new cells , and some cells extend out of the slender pseudopod , and are in a suspended shape , and can extend into the nano - pores which are spaced farther apart .

Conclusion : The surface of titanium alloy with micron - submicron - nanometer three - stage pore structure formed by the method of AC electrolytic etching has good biocompatibility , which can promote the adhesion , proliferation and secretion of cell matrix . The surface of implant neck treated by this method is an excellent surface , which can provide guidance for the subsequent in vivo experiment and implant surface treatment .
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R783.6

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 劉媛媛;游夢;王虎;張靜;楊振宇;林穎潔;繆競威;;納米顆粒鈦膜的構(gòu)建及其表面形貌特征研究[J];華西口腔醫(yī)學(xué)雜志;2009年04期

2 張曉潔;孟維艷;柳忠豪;許勝;;不同鈦表面形貌對人牙齦成纖維細(xì)胞生物學(xué)行為的影響[J];中國口腔種植學(xué)雜志;2011年01期

3 趙嘉惠;張華屏;王春芳;;MTT法在檢測細(xì)胞增殖方面的探討[J];山西醫(yī)科大學(xué)學(xué)報;2007年03期

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