本文選題：咬合干擾 + 咀嚼肌�。� 參考：《廣西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的建立大鼠單側(cè)咬合紊亂的慢性咬合干擾模型,通過(guò)免疫組織化學(xué)方法,觀察大鼠咬合干擾疼痛中腦N/OFQ(Nociceptin/Orphanin FQ)表達(dá)的變化,初步研究其在咬合干擾致咀嚼肌疼痛的中樞作用機(jī)制。方法本實(shí)驗(yàn)取8周齡SD大鼠24只,其中實(shí)驗(yàn)組建模共18只,在左側(cè)上頜第一、二磨牙之間插入一正畸用橡皮筋(3 M U n i t e k,1/8#),使第一磨牙推向近中,逐漸形成上、下頜第一磨牙尖窩不吻合的咬合關(guān)系,實(shí)驗(yàn)期間每天檢查橡皮筋有無(wú)脫落。對(duì)照組6只不施加咬合干擾,相同的狀態(tài)下飼養(yǎng)。模型建立第5天,10天,21天后,隨機(jī)抽取實(shí)驗(yàn)組各6只大鼠及第21天時(shí)對(duì)照組6只大鼠測(cè)量咀嚼肌痛閾值,然后麻醉灌注,取腦組織作切片,利用免疫組織化學(xué)方法檢測(cè)大鼠中腦部分核團(tuán)內(nèi)N/OFQ的表達(dá)情況,初步探討孤啡肽表達(dá)的改變與大鼠咬合干擾疼痛之間的關(guān)系。結(jié)果1.對(duì)照組的痛閾值為208.9±13,模型組第5天、10天、21天的痛閾值分別為117.8±11,73.3±10,198.7±6。2.免疫組化半定量分析結(jié)果顯示,孤啡肽在對(duì)照組第21天時(shí)的平均光密度的表達(dá)為0.184±0.007;在模型組第5天、10天、21天的表達(dá)分別為0.253±0.008,0.326±0.010,0.203±0.004。任意兩組比較差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。孤啡肽在模型組中的表達(dá)均顯著高于對(duì)照組,在模型組第10天的表達(dá)最高,在第21天的表達(dá)較第5天及第10天降低。結(jié)論咬合干擾模型中咀嚼肌的疼痛可促進(jìn)大鼠腦內(nèi)孤啡肽的表達(dá);咀嚼肌痛覺(jué)敏感的程度與大鼠腦內(nèi)孤啡肽的表達(dá)程正相關(guān)。
[Abstract]:Objective to establish a chronic occlusal interference model of unilateral occlusal disturbance in rats and to observe the changes in the expression of N/OFQ (Nociceptin/Orphanin FQ) in the pain of occlusal interference in rats by immunohistochemical method. The mechanism of the central action of the occlusal interference in the pain of masticatory muscles caused by occlusal interference was preliminarily studied. Methods 24 rats of 8 weeks old were taken from this experiment. The experiment was carried out in the experiment. A total of 18 models were set up, and a orthodontic rubber band (3 M U n i t e K, 1/8#) was inserted between the maxillary maxillary molars and the first molar was pushed to the near middle, and the occlusal relationship of the first molar of the mandibular first molar was gradually formed. During the experimental period, the rubber band was checked out. In the control group, 6 only did not apply occlusal interference, and the same state was in the same state. The model was set up for fifth days, 10 days and 21 days, and 6 rats in the experimental group were randomly selected and 6 rats in the control group were randomly selected to measure the threshold of masticatory myalgia, and then the brain tissue was taken to slice. The expression of N/OFQ in the middle cerebral nucleus of the rat was detected by immunohistochemistry. The changes of the expression of orphanin enkephalin were preliminarily discussed. The relationship between the pain of rat occlusion and pain was found. Results the pain threshold value of the 1. control group was 208.9 + 13, the model group was fifth days, 10 days, and the 21 day pain threshold was 117.8 + 11,73.3 + 6.2. immunohistochemical semi quantitative analysis results showed that the average light density of orphan enkephalin was 0.184 + 0.007 in the control group for twenty-first days, while in the model group, 10 days, 10 The expression of 21 days and 21 days was 0.253 + 0.008,0.326 + 0.010,0.203 + 0.004., and there were statistically significant differences between the two groups (P0.01). The expression of orphan enkephalin in the model group was significantly higher than that in the control group. The expression in the model group was the highest at tenth days, and the expression in the twenty-first day was lower than that in the fifth day and the 10 day. Pain can promote the expression of orphan enkephalin in rat brain, and the degree of masticatory myalgia sensitivity is positively correlated with the expression of orphan FQ in rat brain.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R783.5;R-332

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本文編號(hào)：1973197