本文選題：口腔鱗狀細(xì)胞癌 + 凋亡抑制蛋白Livin��； 參考：《安徽醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的： 口腔鱗狀細(xì)胞癌(oral squamous cell carcinima, OSCC)惡性程度高,浸潤(rùn)性強(qiáng),且易發(fā)生淋巴結(jié)及遠(yuǎn)處臟器轉(zhuǎn)移。目前針對(duì)OSCC的研究較多的集中于細(xì)胞凋亡和增殖的平衡機(jī)制方面,Livin和Caspase-3是細(xì)胞凋亡和增殖過(guò)程中重要的生物學(xué)指標(biāo)。本實(shí)驗(yàn)通過(guò)探討凋亡抑制蛋白(IAP) Livin和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)在口腔鱗狀細(xì)胞癌(OSCC)中的表達(dá)及其相關(guān)性,從分子學(xué)水平研究Livin和Caspase-3mRNA在口腔鱗狀細(xì)胞癌(OSCC)和正常口腔黏膜組織(NOT)中的表達(dá),分析二者在口腔鱗狀細(xì)胞癌中表達(dá)的相關(guān)性,為對(duì)患者診斷及監(jiān)測(cè)、評(píng)估術(shù)后復(fù)發(fā)、轉(zhuǎn)移的風(fēng)險(xiǎn)程度提供參考,以輔助臨床工作。 方法： 本研究應(yīng)用逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)(reverse transcription polymerase chain reaction, RT-PCR)方法,選取2011年10月～2013年2月在安徽醫(yī)科大學(xué)第一附屬醫(yī)院口腔頜面外科就診,并均經(jīng)組織病理檢查確診為OSCC患者新鮮組織標(biāo)本45例,所有OSCC組患者均為首次確診,術(shù)前均未行放、化療或其他抗腫瘤治療,均無(wú)遠(yuǎn)端臟器的轉(zhuǎn)移,無(wú)其他惡性腫瘤史,無(wú)家族腫瘤史。另選11例患者為陰性對(duì)照組(NOT組),其中7例取自阻生齒拔除患者,4例取自牙槽嵴修整患者。所有標(biāo)本均于術(shù)中取材后立即置于液氮中,然后移至-80℃超低溫冰箱中貯存?zhèn)溆谩?結(jié)果： 1.Livin mRNA和Caspase-3mRNA在OSCC組織中的相對(duì)含量均與組織分化程度、淋巴結(jié)轉(zhuǎn)移和臨床分期均有相關(guān)性；但與患者的年齡、性別無(wú)關(guān)。 2.Livin和Caspase-3在45例OSCC和11例NOT中均有不同程度的表達(dá)：Caspase-3mRNA在OSCC組織中的陽(yáng)性表達(dá)率明顯低于NOT (P0.05);其在NOT中相對(duì)含量(0.193±0.031),高于OSCC(0.102±0.025)(P0.01)。Livin mRNA在OSCC組織中的陽(yáng)性表達(dá)率明顯高于NOT (P0.05);其表達(dá)量在OSCC組織中為(0.542±0.074),明顯高于NOT (0.181±0.026)(P0.01). 3.根據(jù)Pearson列聯(lián)系數(shù)相關(guān)分析, Livin mRNA和Caspase-3mRNA在OSCC組織中的表達(dá)呈相關(guān)關(guān)系(P0.01)。 結(jié)論： Livin和Caspase-3在OSCC中的表達(dá)結(jié)果分析顯示,兩者可能參與了OSCC的發(fā)生、發(fā)展的過(guò)程,聯(lián)合檢測(cè)Livin與Caspase-3可以為研究OSCC發(fā)病機(jī)制提供新的思路,為OSCC的臨床分級(jí)、分期、評(píng)估患者預(yù)后提供數(shù)據(jù)支持。
[Abstract]:Objective: Oral squamous cell carcinima, OSCC) in oral squamous cell carcinoma is highly malignant, invasive and prone to lymph node and distant organ metastasis. At present, the researches on OSCC focus on the balance mechanism of apoptosis and proliferation. Livin and Caspase-3 are important biological indexes in the process of cell apoptosis and proliferation. In this study, we investigated the expression of Livin and caspase-3 in oral squamous cell carcinoma (OSCC) and their correlation with the expression of apoptosis-inhibiting protein (IAP) and cysteine aspartate proteinase-3 (Caspase-3) in oral squamous cell carcinoma (OSCC). To study the expression of Livin and Caspase-3mRNA in oral squamous cell carcinoma (OSCC) and normal oral mucosa tissue from molecular level, and to analyze the correlation between them in oral squamous cell carcinoma (OSCC) and normal oral mucosa. The degree of risk of transfer provides a reference to assist clinical work. Methods: In this study, reverse transcription polymerase chain reaction, RT-PCR method was used to select oral and maxillofacial surgery patients from October 2011 to February 2013 in the first affiliated Hospital of Anhui Medical University. 45 cases of fresh tissue specimens were confirmed by histopathological examination. All the patients in OSCC group were diagnosed for the first time. No radiotherapy, chemotherapy or other antitumor therapy, no metastasis of distal organs and no history of other malignant tumors were performed before operation. No family history of cancer. Another 11 cases were selected as negative control group, 7 cases were extracted from impacted teeth, 4 cases were from alveolar ridge dressing. All specimens were collected during operation and placed in liquid nitrogen immediately, then moved to-80 鈩，

本文編號(hào)：1939475