Nd:YAG激光對(duì)牙根面結(jié)構(gòu)及變異鏈球菌黏附的影響
發(fā)布時(shí)間:2018-05-26 12:42
本文選題:Nd + YAG激光; 參考:《華西口腔醫(yī)學(xué)雜志》2016年06期
【摘要】:目的研究不同功率的水冷Nd:YAG激光照射治療對(duì)牙根面結(jié)構(gòu)及變異鏈球菌黏附的影響。方法將因重度慢性牙周炎拔除的離體牙分為4組,均進(jìn)行手工刮治和根面平整,制備牙根片,激光1、2、3組分別用功率為4、6、8 W的水冷Nd:YAG激光照射處理60 s,對(duì)照組不作處理。用掃描電子顯微鏡(SEM)觀察各組根片的表面結(jié)構(gòu)。將變異鏈球菌接種于各組牙根片表面并進(jìn)行培養(yǎng),計(jì)數(shù)各組根片表面細(xì)菌的黏附量(CFU·m L-1);同時(shí)用SEM觀察對(duì)照組及激光2組根面的細(xì)菌黏附情況。結(jié)果 SEM觀察根片表面結(jié)構(gòu),激光組較對(duì)照組牙根表面的玷污層、碎屑及菌斑樣物質(zhì)附著減少,但裂隙增多。3個(gè)激光組牙根表面的細(xì)菌黏附量均少于對(duì)照組(P0.05),而不同功率的激光組之間的差異無統(tǒng)計(jì)學(xué)意義(P0.05)。SEM觀察細(xì)菌黏附情況,激光2組表面的細(xì)菌黏附量明顯少于對(duì)照組。結(jié)論與單純刮治相比,刮治后進(jìn)行水冷Nd:YAG激光治療能清除牙根表面的玷污層、碎屑及菌斑樣物質(zhì),并能減少變異鏈球菌的黏附,但激光會(huì)導(dǎo)致牙根表面出現(xiàn)裂隙。本實(shí)驗(yàn)中,6 W為激光最佳功率,既能較好地去除牙根表面的玷污層和碎屑等,又能較好地控制激光對(duì)根面的熱損害程度。
[Abstract]:Objective to study the effects of water-cooled Nd:YAG laser irradiation on root surface structure and adhesion of Streptococcus mutans. Methods the extracted teeth of severe chronic periodontitis were divided into 4 groups. Root slices were prepared by manual curettage and root surface leveling. The rats in the laser 1 / 2 group were treated with water cooled Nd:YAG laser for 60 s, respectively, and the control group was not treated. The surface structure of root slices was observed by scanning electron microscope (SEM). Streptococcus mutans were inoculated on the root surface of each group and cultured, and the amount of bacterial adhesion on the surface of each group was counted. Meanwhile, SEM was used to observe the bacterial adhesion on the root surface of the control group and the laser group. Results the surface structure of root slices was observed by SEM. Compared with the control group, the smear layer, debris and plaque adhesion of the laser group were less than those of the control group. However, the number of bacteria adhesion on the root surface of the three laser groups was lower than that of the control group (P 0.05), but there was no significant difference between the different laser groups (P 0.05). SEM was used to observe the bacterial adhesion. The bacterial adhesion on the surface of the two laser groups was significantly lower than that of the control group. Conclusion compared with simple curettage, water-cooled Nd:YAG laser can remove smear layer, debris and plaque like substance on root surface, and reduce adhesion of Streptococcus mutans, but laser can cause crack on root surface. In this experiment, 6W is the best laser power, which can not only remove the smear layer and debris on the root surface, but also control the thermal damage degree of the root surface.
【作者單位】: 口腔疾病研究國家重點(diǎn)實(shí)驗(yàn)室華西口腔醫(yī)院牙周病科(四川大學(xué));
【分類號(hào)】:R781
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本文編號(hào):1937269
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