本文選題：偏側咀嚼 + 髁突軟骨��； 參考：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：[目的]探討偏側咀嚼對SD大鼠髁突軟骨中DMP1表達的影響。[方法]通過拔除8周齡SD雄鼠右側上頜所有磨牙建立偏側咀嚼動物模型,于術后2w、4w、6w、8w取材(髁突),常規(guī)固定,脫鈣,脫水,包埋、石蠟切片和染色。HE染色:觀察髁突軟骨及軟骨下骨組織學變化,測量髁突軟骨厚度,進行髁突軟骨下骨骨形態(tài)計量分析(BV/TV、Tb.Th、Tb.N、Tb.Sp);免疫組織化學方法檢測不同時間點SD大鼠髁突軟骨中DMP1的表達變化。采用Image Pro Plus 6.0圖像分析系統(tǒng)進行測量分析。所有數(shù)據(jù)均應用SPSS23.0統(tǒng)計軟件進行統(tǒng)計學分析。[結果]1、HE染色顯示實驗組髁突軟骨及軟骨下骨都出現(xiàn)了病理性改變,表現(xiàn)為軟骨厚度降低,軟骨下骨骨小梁排列紊亂。髁突軟骨厚度測量結果顯示:術后4w、6w、8w實驗組軟骨厚度小于對照組(P0.05),拔牙側與非拔牙側組差異無統(tǒng)計學意義(P0.05);髁突軟骨下骨骨形態(tài)計量分析結果顯示:BV/TV在術后2w、4w、6w拔牙側、非拔牙側均低于對照組(P0.05);Tb.Th、Tb.N在術后2w拔牙側、非拔牙側均低于對照組(P0.05);Tb.Sp在術后2w、4w拔牙側、非拔牙側均高于對照組(P0.05)。拔牙側與非拔牙側各軟骨下骨參數(shù)差異無統(tǒng)計學意義(P0.05)。2、免疫組化結果顯示:實驗組與對照組髁突軟骨的DMP1都呈陽性表達,表達于成軟骨細胞層和肥大細胞層的細胞核及周圍胞質。實驗組中拔牙側、非拔牙側DMP1表達在術后2w、4w、6w、8w都高于對照組(P0.05)。拔牙側與非拔牙側DMP1表達的差異無統(tǒng)計學意義(P0.05)。拔牙側DMP1表達在術后2w與4w、4w與8w,非拔牙側DMP1表達在術后2w與4w、4w與6w的差異有統(tǒng)計學意義(P0.05)。[結論]1、通過拔牙法建立的偏側咀嚼會導致實驗組SD大鼠拔牙側和非拔牙側髁突軟骨和髁突軟骨下骨出現(xiàn)病理性改變。髁突軟骨厚度變薄,雙側髁突軟骨下骨骨小梁數(shù)目、形態(tài)結構的異常以及排列的紊亂。拔牙側與非拔牙側髁突軟骨及軟骨下骨病理改變無差異。2、DMP1參與髁突軟骨的生長發(fā)育,主要表達在髁突軟骨成軟骨細胞層和肥大層的細胞核及細胞質;偏側咀嚼導致髁突軟骨發(fā)生病理性改建,實驗組髁突軟骨DMP1陽性表達高于對照組,說明DMP1參與偏側咀嚼引起的髁突軟骨改建。
[Abstract]:[objective] to investigate the effect of unilateral mastication on the expression of DMP1 in condylar cartilage of SD rats. [methods] the animal model of unilateral mastication was established by pulling out all the right maxillary molars of 8-week-old SD rats. The mandibular condyle was fixed, decalcified, dehydrated and embedded at 2 weeks, 4 weeks, 6 weeks and 8 weeks after operation. Paraffin sections and staining. HE staining: the histological changes of condylar cartilage and subchondral bone were observed and the thickness of condylar cartilage was measured. The bone morphology of subcondylar cartilage was measured and the expression of DMP1 in condylar cartilage of SD rats at different time points was detected by immunohistochemical method. Image Pro Plus 6.0 image analysis system is used for measurement and analysis. All the data were analyzed by SPSS23.0 statistical software. [results] 1HE staining showed that there were pathological changes in condylar cartilage and subchondral bone in the experimental group, which showed that the thickness of cartilage decreased and the trabecular arrangement of subchondral bone was disordered. The thickness measurement of condylar cartilage showed that the thickness of cartilage in the experimental group was less than that in the control group at 4 weeks and 6 weeks after operation, but there was no significant difference between the extraction side and the non-extraction side. The non-extraction side was lower than that of the control group (P 0.05), and the non-extraction side was lower than that of the control group (P 0.05) and the non-extraction side was higher than that of the control group (P 0.05) at 2 and 4 weeks after the operation, and the non-extraction side was higher than that of the control group (P 0.05). There was no significant difference in the parameters of subchondral bone between the extraction side and the non-extraction side. The immunohistochemical results showed that the DMP1 expression was positive in condylar cartilage of the experimental group and the control group. Expressed in the nuclei and surrounding cytoplasm of chondroblasts and mast cell layers. In the experimental group, the expression of DMP1 in the extraction side and non-extraction side was significantly higher than that in the control group at 2, 4, 4, 6 and 8 weeks postoperatively. There was no significant difference in the expression of DMP1 between the extraction side and the non-extraction side (P 0.05). The expression of DMP1 in the extraction side was significantly higher than that in the non-extraction side at 2 and 4 weeks after extraction and at 4 and 8 weeks postoperatively, and there was a significant difference in the expression of DMP1 between 2 and 4 weeks after extraction and at 4 and 6 weeks postoperatively (P 0.05). [conclusion] 1. Unilateral mastication established by extraction method may lead to pathological changes of condylar cartilage and subcondylar cartilage of SD rats in experimental group. The thickness of condylar cartilage thinned, the number of bone trabeculae of bilateral condylar cartilage, the abnormality of morphology and structure, and the disorder of arrangement. There was no difference in pathological changes of condylar cartilage and subchondral bone between extraction side and non-extraction side. 2DMP1 was involved in the growth and development of condylar cartilage, mainly expressed in the nucleus and cytoplasm of chondroblast layer and hypertrophic layer of condylar cartilage. Unilateral mastication resulted in pathological remodeling of condylar cartilage. The positive expression of DMP1 in condylar cartilage in experimental group was higher than that in control group, indicating that DMP1 was involved in condylar cartilage remodeling induced by unilateral mastication.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R782.6

【參考文獻】

相關期刊論文 前10條

1 李雪;王明國;楊帥;高潔;范園園;宋巖;;下頜持續(xù)前導作用下髁狀突軟骨改建的超微結構變化[J];中國組織工程研究;2015年29期

2 黃林劍;蔡協(xié)藝;李輝;盛夏涵;楊馳;;兔關節(jié)盤、髁突及軟骨下骨的年齡相關性改變組織學研究[J];口腔頜面外科雜志;2015年01期

3 伍虹;莊沛林;余艷崧;陳絳媛;Sfeir Charles;黃洪章;;MAPK-ERK在牙本質基質蛋白1信號通路中的作用[J];中華口腔醫(yī)學研究雜志(電子版);2013年06期

4 劉瑩;湯歡;劉艷艷;楊午;劉暢;;不同下頜前伸力對髁突Runx2和X型膠原表達的影響[J];實用口腔醫(yī)學雜志;2013年05期

5 丁婷婷;王子嫻;劉釗;林雪芬;祁冬;汲平;;偏側咀嚼不同時期大鼠咬肌纖維類型變化的研究[J];廣東牙病防治;2010年12期

6 姜華;王照五;劉洪臣;胡敏;曹均凱;石校偉;;右側偏側咀嚼對顳下頜關節(jié)形態(tài)的影響[J];上�？谇会t(yī)學;2010年06期

7 許躍;陳揚熙;;顳下頜關節(jié)軟骨下骨改建[J];國際口腔醫(yī)學雜志;2008年01期

8 郭維華;李松;徐蕓;;不同靜壓力對大鼠髁突軟骨細胞生物學特性的影響[J];華西口腔醫(yī)學雜志;2007年06期

9 王冠超;劉洪臣;馬軍利;王東勝;鄂玲玲;;咀嚼力降低對發(fā)育期大鼠髁突發(fā)育的組織學影響[J];口腔頜面修復學雜志;2007年02期

10 吳莉萍;凌均h，

本文編號：1913710