本文選題：偏側(cè)咀嚼 + 髁突軟骨��； 參考：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：[目的]探討偏側(cè)咀嚼對(duì)SD大鼠髁突軟骨中DMP1表達(dá)的影響。[方法]通過拔除8周齡SD雄鼠右側(cè)上頜所有磨牙建立偏側(cè)咀嚼動(dòng)物模型,于術(shù)后2w、4w、6w、8w取材(髁突),常規(guī)固定,脫鈣,脫水,包埋、石蠟切片和染色。HE染色:觀察髁突軟骨及軟骨下骨組織學(xué)變化,測量髁突軟骨厚度,進(jìn)行髁突軟骨下骨骨形態(tài)計(jì)量分析(BV/TV、Tb.Th、Tb.N、Tb.Sp);免疫組織化學(xué)方法檢測不同時(shí)間點(diǎn)SD大鼠髁突軟骨中DMP1的表達(dá)變化。采用Image Pro Plus 6.0圖像分析系統(tǒng)進(jìn)行測量分析。所有數(shù)據(jù)均應(yīng)用SPSS23.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。[結(jié)果]1、HE染色顯示實(shí)驗(yàn)組髁突軟骨及軟骨下骨都出現(xiàn)了病理性改變,表現(xiàn)為軟骨厚度降低,軟骨下骨骨小梁排列紊亂。髁突軟骨厚度測量結(jié)果顯示:術(shù)后4w、6w、8w實(shí)驗(yàn)組軟骨厚度小于對(duì)照組(P0.05),拔牙側(cè)與非拔牙側(cè)組差異無統(tǒng)計(jì)學(xué)意義(P0.05);髁突軟骨下骨骨形態(tài)計(jì)量分析結(jié)果顯示:BV/TV在術(shù)后2w、4w、6w拔牙側(cè)、非拔牙側(cè)均低于對(duì)照組(P0.05);Tb.Th、Tb.N在術(shù)后2w拔牙側(cè)、非拔牙側(cè)均低于對(duì)照組(P0.05);Tb.Sp在術(shù)后2w、4w拔牙側(cè)、非拔牙側(cè)均高于對(duì)照組(P0.05)。拔牙側(cè)與非拔牙側(cè)各軟骨下骨參數(shù)差異無統(tǒng)計(jì)學(xué)意義(P0.05)。2、免疫組化結(jié)果顯示:實(shí)驗(yàn)組與對(duì)照組髁突軟骨的DMP1都呈陽性表達(dá),表達(dá)于成軟骨細(xì)胞層和肥大細(xì)胞層的細(xì)胞核及周圍胞質(zhì)。實(shí)驗(yàn)組中拔牙側(cè)、非拔牙側(cè)DMP1表達(dá)在術(shù)后2w、4w、6w、8w都高于對(duì)照組(P0.05)。拔牙側(cè)與非拔牙側(cè)DMP1表達(dá)的差異無統(tǒng)計(jì)學(xué)意義(P0.05)。拔牙側(cè)DMP1表達(dá)在術(shù)后2w與4w、4w與8w,非拔牙側(cè)DMP1表達(dá)在術(shù)后2w與4w、4w與6w的差異有統(tǒng)計(jì)學(xué)意義(P0.05)。[結(jié)論]1、通過拔牙法建立的偏側(cè)咀嚼會(huì)導(dǎo)致實(shí)驗(yàn)組SD大鼠拔牙側(cè)和非拔牙側(cè)髁突軟骨和髁突軟骨下骨出現(xiàn)病理性改變。髁突軟骨厚度變薄,雙側(cè)髁突軟骨下骨骨小梁數(shù)目、形態(tài)結(jié)構(gòu)的異常以及排列的紊亂。拔牙側(cè)與非拔牙側(cè)髁突軟骨及軟骨下骨病理改變無差異。2、DMP1參與髁突軟骨的生長發(fā)育,主要表達(dá)在髁突軟骨成軟骨細(xì)胞層和肥大層的細(xì)胞核及細(xì)胞質(zhì);偏側(cè)咀嚼導(dǎo)致髁突軟骨發(fā)生病理性改建,實(shí)驗(yàn)組髁突軟骨DMP1陽性表達(dá)高于對(duì)照組,說明DMP1參與偏側(cè)咀嚼引起的髁突軟骨改建。
[Abstract]:[objective] to investigate the effect of unilateral mastication on the expression of DMP1 in condylar cartilage of SD rats. [methods] the animal model of unilateral mastication was established by pulling out all the right maxillary molars of 8-week-old SD rats. The mandibular condyle was fixed, decalcified, dehydrated and embedded at 2 weeks, 4 weeks, 6 weeks and 8 weeks after operation. Paraffin sections and staining. HE staining: the histological changes of condylar cartilage and subchondral bone were observed and the thickness of condylar cartilage was measured. The bone morphology of subcondylar cartilage was measured and the expression of DMP1 in condylar cartilage of SD rats at different time points was detected by immunohistochemical method. Image Pro Plus 6.0 image analysis system is used for measurement and analysis. All the data were analyzed by SPSS23.0 statistical software. [results] 1HE staining showed that there were pathological changes in condylar cartilage and subchondral bone in the experimental group, which showed that the thickness of cartilage decreased and the trabecular arrangement of subchondral bone was disordered. The thickness measurement of condylar cartilage showed that the thickness of cartilage in the experimental group was less than that in the control group at 4 weeks and 6 weeks after operation, but there was no significant difference between the extraction side and the non-extraction side. The non-extraction side was lower than that of the control group (P 0.05), and the non-extraction side was lower than that of the control group (P 0.05) and the non-extraction side was higher than that of the control group (P 0.05) at 2 and 4 weeks after the operation, and the non-extraction side was higher than that of the control group (P 0.05). There was no significant difference in the parameters of subchondral bone between the extraction side and the non-extraction side. The immunohistochemical results showed that the DMP1 expression was positive in condylar cartilage of the experimental group and the control group. Expressed in the nuclei and surrounding cytoplasm of chondroblasts and mast cell layers. In the experimental group, the expression of DMP1 in the extraction side and non-extraction side was significantly higher than that in the control group at 2, 4, 4, 6 and 8 weeks postoperatively. There was no significant difference in the expression of DMP1 between the extraction side and the non-extraction side (P 0.05). The expression of DMP1 in the extraction side was significantly higher than that in the non-extraction side at 2 and 4 weeks after extraction and at 4 and 8 weeks postoperatively, and there was a significant difference in the expression of DMP1 between 2 and 4 weeks after extraction and at 4 and 6 weeks postoperatively (P 0.05). [conclusion] 1. Unilateral mastication established by extraction method may lead to pathological changes of condylar cartilage and subcondylar cartilage of SD rats in experimental group. The thickness of condylar cartilage thinned, the number of bone trabeculae of bilateral condylar cartilage, the abnormality of morphology and structure, and the disorder of arrangement. There was no difference in pathological changes of condylar cartilage and subchondral bone between extraction side and non-extraction side. 2DMP1 was involved in the growth and development of condylar cartilage, mainly expressed in the nucleus and cytoplasm of chondroblast layer and hypertrophic layer of condylar cartilage. Unilateral mastication resulted in pathological remodeling of condylar cartilage. The positive expression of DMP1 in condylar cartilage in experimental group was higher than that in control group, indicating that DMP1 was involved in condylar cartilage remodeling induced by unilateral mastication.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R782.6

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