本文選題：五倍子水提取物 + 體外培養(yǎng)　； 參考：《牙體牙髓牙周病學(xué)雜志》2014年11期

【摘要】：目的:觀察五倍子水提取物對人牙髓細胞Ⅰ型膠原合成的影響。方法:取第6代體外培養(yǎng)的人牙髓細胞分別與終末濃度為5、10μg/m L的五倍子水提取物共同培養(yǎng)3 d后,采用免疫組化染色法觀察人牙髓細胞中Ⅰ型膠原的表達情況,并用HPIAS-1000圖像分析系統(tǒng)進行定量分析。結(jié)果:對照組和不同濃度五倍子水提取物作用的實驗組人牙髓細胞中均有Ⅰ型膠原的表達,其免疫反應(yīng)物質(zhì)多定位于胞漿。人牙髓細胞經(jīng)5、10μg/m L濃度的五倍子水提取物作用后,其Ⅰ型膠原的表達水平均明顯增高,其中以10μg/m L組的增高程度最大,各組間兩兩相比差異均有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:5、10μg/m L五倍子水提取物均能明顯促進人牙髓細胞中Ⅰ型膠原的表達;提示其具有促進牙髓細胞向成牙本質(zhì)細胞分化的作用。
[Abstract]:Aim: to observe the effect of gallnut water extract on collagen type I synthesis in human dental pulp cells. Methods: human dental pulp cells cultured in vitro were cultured with 5 渭 g / mL gallnut water extract (5 渭 g / mL) for 3 days. The expression of type I collagen in human dental pulp cells was observed by immunohistochemical staining. Quantitative analysis was carried out with HPIAS-1000 image analysis system. Results: the expression of type I collagen was found in human dental pulp cells in the control group and in the experimental group treated with different concentrations of gallnut water extract. The immunoreactive substances were mostly localized in the cytoplasm. The expression of type 鈪，

本文編號：1895141