發(fā)布時間：2018-05-15 22:34

  本文選題：低氧 + 人牙囊細胞　； 參考：《華西口腔醫(yī)學雜志》2017年03期

【摘要】：目的研究低氧對人牙囊細胞(h DFCs)生物學特性的影響。方法利用組織塊酶消化法從年輕恒牙中分離培養(yǎng)h DFCs;采用免疫熒光技術(shù)檢測細胞表面標志物,多向誘導實驗檢測細胞多向分化潛能;模擬體外低氧微環(huán)境,將細胞分為常氧組(20%O2)和低氧組(2%O2),分別對兩組細胞行Transwell小室試驗檢測低氧對細胞遷移的影響,采用CCK-8法檢測低氧對細胞增殖的影響。通過實時定量聚合酶鏈反應(q RT-PCR)和Western blot分別從基因和蛋白水平檢測h DFCs多能性相關(guān)標志物于不同氧體積分數(shù)下的表達;分別對兩組細胞進行成骨誘導,q RT-PCR檢測成骨相關(guān)基因,茜素紅染色評估礦化結(jié)節(jié)的形成。結(jié)果 h DFCs具有較強的干細胞特征,具有成骨、成脂及成神經(jīng)多向分化能力,符合間充質(zhì)干細胞基本標準,能夠滿足牙組織工程構(gòu)建對種子細胞的需求。低氧有利于h DFCs多能性的保持,同時促進了h DFCs的遷移和增殖。h DFCs于低氧中進行誘導時,其成骨分化能力得到增強。結(jié)論低氧微環(huán)境對維持h DFCs多能性,促進h DFCs增殖、遷移和分化有重要作用。
[Abstract]:Objective to study the effects of hypoxia on the biological characteristics of human dental follicle cells (H DFCs). Methods h DFCs was isolated and cultured from young permanent teeth by tissue block enzyme digestion. Immunofluorescence was used to detect cell surface markers and multidirectional induction test was used to detect the multidirectional differentiation potential of the cells; the hypoxic microenvironment was modeled in vitro, and the cells were divided into the normal oxygen group (20%O2). The effect of hypoxia on cell migration was detected in two groups of cells in two groups of Transwell cells, and the effect of hypoxia on cell proliferation was detected by CCK-8 method. The different oxygen volumes of H DFCs multipotent markers were detected by real-time quantitative polymerase chain reaction (Q RT-PCR) and Western blot, respectively. Two groups of cells were induced to induce osteogenesis, Q RT-PCR was used to detect bone related genes, and alizarin red staining was used to evaluate the formation of mineralized nodules. Results h DFCs had strong stem cell characteristics, with osteogenesis, lipid and neurogenic multidirectional differentiation, which met the basic standard of mesenchymal stem cells and could meet the construction of dental tissue engineering. The demand for seed cells. Hypoxia is beneficial to the retention of H DFCs pluriability, while promoting the migration and proliferation of H DFCs, the osteogenic differentiation ability of.H DFCs is enhanced when.H DFCs is induced in hypoxia. Conclusion hypoxia microenvironment plays an important role in maintaining h DFCs multienergy, promoting h DFCs proliferation, migration and differentiation.

【作者單位】： 口腔疾病研究國家重點實驗室國家口腔疾病臨床研究中心四川大學華西口腔醫(yī)院創(chuàng)傷與整形外科;口腔再生醫(yī)學國家地方聯(lián)合工程實驗室華西口腔醫(yī)院(四川大學);
【基金】：國家自然科學基金(81271119)~~
【分類號】：R781

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