本文選題：根管封閉劑 + 細胞毒性��； 參考：《武漢大學(xué)》2014年博士論文

【摘要】：第一部分MetaSEAL, RealSeal SE對MC3T3-E1成骨細胞的細胞毒性 目的：評價兩種新型自粘結(jié)樹脂根管封閉劑MetaSEAL和RealSeal SE對MC3T3-E1成骨細胞的細胞毒性,并與傳統(tǒng)的環(huán)氧樹脂根管封閉劑AH plus做對比。 方法：受試各組根管封閉劑注入Teflon管(直徑4.8mm,高2mm)中,固化7天后加入培養(yǎng)基浸提。浸提液用培養(yǎng)基倍比稀釋為4種濃度：100%、50%、25%、12.5%,MC3T3-E1細胞于其中培養(yǎng),分別于24h和72h后取出,用CCK-8法檢測細胞增殖活性,計算細胞存活率。采用單因素方差分析和Bonferroni多重法,比較在相同的稀釋濃度下,不同根管封閉劑的細胞存活率差異；采用t-檢驗,比較同種浸提液在高濃度(100%、50%)和低濃度(25%、12.5%)下的細胞存活率差異。 結(jié)果：MetaSEAL組細胞存活率均顯著低于RealSeal SE和AH Plus (p0.05).100%、50%、25%RealSeal SE浸提液的細胞存活率顯著低于AH Plus (PO.05),12.5%濃度下存活率與AH Plus相似。AH Plus的細胞存活率在100%、50%濃度時與陰性對照組無差異,在25%、12.5%濃度下均顯著高于陰性對照組(P0.05)。RealSeal SE低濃度組的細胞存活率顯著高于高濃度組(P0.05),MetaSEAL和AH Plus組的細胞存活率在高低濃度組間無顯著差異。 結(jié)論：在本實驗條件下,MetaSEAL對成骨細胞表現(xiàn)出嚴重的細胞毒性；RealSealSE在釋放物濃度高時毒性強,濃度低時無毒性；而AH Plus基本無毒性。 第二部分MetaSEAL和RealSeal SE與牙根牙本質(zhì)的推出粘結(jié)強度 目的：評價兩種新型自粘結(jié)樹脂根管封閉劑(MetaSEAL, RealSeal SE)與牙根牙本質(zhì)的粘結(jié)強度。 方法：單根管牙36顆,沿頰舌向縱剖為厚度為0.9mm的牙片,在根中部牙本質(zhì)內(nèi)制備體積基本相同的模擬根管,5.25%次氯酸鈉液、17%EDTA溶液超聲沖洗。牙片隨機分三組,每組12顆,于模擬根管內(nèi)分別充填MetaSEAL, RealSeal SE和AH Plus,固化一周。每組取10個牙片20個孔(n=20)進行薄片推出試驗。采用Kruskal-Wallis analysis檢驗和Mann-Whitney檢驗各組的粘結(jié)強度,體視顯微鏡下觀察粘結(jié)界面斷裂類型和微觀形態(tài)。剩余2個牙片部分脫礦,用于掃描電鏡(SEM)下觀察樹脂突微觀形態(tài)。 結(jié)果： 1, MetaSEAL的粘結(jié)強度(12.01+4.66MPa)顯著高于其他組(P0.05); RealSeal SE(5.43±3.68MPa)與AH Plus (7.34±2.83MPa)的粘結(jié)強度無顯著性差異,MetaSEAL和AH Plus粘結(jié)界面斷裂的主要類型為混合失敗,而RealSeal SE為粘結(jié)失敗。 2、SEM鏡下可見各組樣本牙本質(zhì)壁上均有數(shù)量不等的根管封閉劑殘留和牙本質(zhì)小管開口,有的小管內(nèi)見折斷的樹脂突。以MetaSEAL組殘留的封閉劑和折斷的樹脂突最多,RealSeal SE組最少。各組牙本質(zhì)--樹脂粘結(jié)界面均有裂隙和樹脂突形成。MetaSEAL組可見封閉劑大量滲入牙本質(zhì)小管,形成連續(xù)的、長而密集的樹脂突；AH Plus組樹脂突也較多、較長但不連續(xù)；RealSeal SE樹脂突最短且最少見。 結(jié)論：在完全去除玷污層的情況下,MetaSEAL與牙根牙本質(zhì)的粘結(jié)能力優(yōu)越,RealSeal SE與AH Plus粘結(jié)力相近,在臨床可接受范圍內(nèi)。從材料學(xué)的角度來說,自粘結(jié)樹脂根管封閉劑與牙根牙本質(zhì)的真實粘結(jié)性能良好。
[Abstract]:Part one: cytotoxicity of MetaSEAL, RealSeal SE on MC3T3-E1 osteoblasts
Objective: To evaluate the cytotoxicity of two new self bonded resin root canal sealers MetaSEAL and RealSeal SE on MC3T3-E1 osteoblasts and to compare with the traditional epoxy resin root canal sealant AH plus.
Methods: the root canal sealants were injected into the Teflon tube (diameter 4.8mm, high 2mm), and then added to the medium for 7 days. The extract was diluted to 4 concentrations with medium double dilution: 100%, 50%, 25%, 12.5%, and MC3T3-E1 cells were cultured after 24h and 72h respectively. The cell proliferation activity was detected by CCK-8 method and the cell survival rate was calculated. The cell survival rates of different root canal sealants were compared with the single factor ANOVA and Bonferroni multiple methods, and the cell survival rates of the same extract were compared under high concentration (100%, 50%) and low concentration (25%, 12.5%) by t- test.
Results: the cell survival rate of MetaSEAL group was significantly lower than that of RealSeal SE and AH Plus (P0.05).100%, 50%, the cell survival rate of 25%RealSeal SE leaching solution was significantly lower than AH Plus (PO.05). The survival rate of the cells was similar to that of the negative control group at the concentration of 100% and 50%. The survival rate was not different from that of the negative control group at the concentration of 25% and 12.5%. The cell survival rate in the low concentration group of the P0.05.RealSeal SE group was significantly higher than that in the high concentration group (P0.05). There was no significant difference in the cell survival rate between the MetaSEAL and the AH Plus groups in the high and low concentration groups.
Conclusion: in this experimental condition, MetaSEAL showed severe cytotoxicity to osteoblasts, and RealSealSE was toxic when the concentration of the release was high and the concentration was low, while AH Plus was nontoxic.
The second part is the bond strength between MetaSEAL and RealSeal SE and root dentin.
Objective: To evaluate the bond strength between two new self adhesive resin MetaSEAL (RealSeal SE) and root dentin.
Methods: 36 teeth with single root canal, along the cheek and tongue to the thickness of 0.9mm, were divided into 5.25% groups, 5.25% sodium hypochlorite liquid, and 17%EDTA solution ultrasonic irrigation. The teeth were randomly divided into three groups, each group was filled with MetaSEAL, RealSeal SE and AH Plus in each group. Each group was solidified for one week. 10 dental flakes were taken with 20 holes (n=20) to carry out a thin slice test. The bond strength of each group was examined by Kruskal-Wallis analysis test and Mann-Whitney. The fracture type and Micromorphology of the bonding interface were observed under stereoscopic microscope. The remaining 2 teeth were partially demineralized, and the microstructure of the resin process was observed under the scanning electron microscope (SEM).
Result錛，

本文編號：1845406