本文選題：牙周膜成纖維細(xì)胞 + 低氧　； 參考：《上海口腔醫(yī)學(xué)》2014年04期

【摘要】：目的:觀察低氧對體外培養(yǎng)的人牙周膜成纖維細(xì)胞(periodontal ligament cells,PDLCs)增殖與成骨分化的影響。方法:體外培養(yǎng)鑒定人牙周膜成纖維細(xì)胞,分別用濃度為0、100、200、400μmol/L的CoCl2作用于細(xì)胞,采用MTT法觀察CoCl2對PDLCs增殖的影響,運用實時定量PCR和Western免疫印跡技術(shù)檢測CoCl2模擬低氧對PDLCs成骨分化的影響。采用SPSS13.0軟件包對數(shù)據(jù)進(jìn)行統(tǒng)計學(xué)分析。結(jié)果:免疫組化染色結(jié)果證實,培養(yǎng)細(xì)胞為牙周膜成纖維細(xì)胞。200μmol/L及400μmol/L的CoCl2均能抑制PDLCs的增殖及堿性磷酸酶、RUNX2、Ⅰ型膠原的表達(dá),并呈劑量依賴性。結(jié)論:氯化鈷模擬的低氧環(huán)境對PDLCs增殖及成骨分化具有抑制作用。
[Abstract]:Objective: To observe the effects of hypoxia on the proliferation and osteogenic differentiation of human periodontal ligament fibroblasts (periodontal ligament cells (PDLCs)) in vitro. Methods: human periodontal ligament fibroblasts were cultured in vitro, and CoCl2 was used to observe the effect of CoCl2 on PDLCs proliferation by MTT method, and the effect of CoCl2 on PDLCs proliferation was observed by MTT method, and the effect of CoCl2 on PDLCs proliferation was observed by MTT method. Real-time quantitative PCR and Western immunoblotting were used to detect the effect of CoCl2 simulated hypoxia on the osteogenesis of PDLCs. The data were analyzed by SPSS13.0 software package. Results: the results of immunohistochemical staining showed that the proliferation and alkaline phosphorus of the cultured cells of the periodontal ligament fibroblasts.200 mu mol/L and 400 u mol/L could inhibit the proliferation and alkaline phosphorus of PDLCs. The expression of acid enzyme, RUNX2 and type I collagen was dose-dependent. Conclusion: the hypoxic environment simulated by cobalt chloride can inhibit the proliferation and osteogenic differentiation of PDLCs.

【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院·口腔醫(yī)學(xué)院牙周病科;上海市口腔醫(yī)學(xué)研究所 上海市口腔醫(yī)學(xué)重點實驗室;
【基金】：國家自然科學(xué)基金(81070838,81271156) 上海交通大學(xué)醫(yī)工交叉研究基金(YG2011MS31)~~
【分類號】：R781.4

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