本文選題：Kv. + 人牙周膜成纖維細胞��； 參考：《安徽醫(yī)科大學學報》2017年06期

【摘要】：目的探討Kv1.3在人牙周膜成纖維細胞(h PDLFs)增殖和分化成骨能力中的作用。方法通過體外分離并培養(yǎng)健康人和牙周炎患者牙周膜成纖維細胞,應用細胞免疫熒光法、Q-PCR和Western blot檢測Kv1.3在牙周炎h PDLFs中表達變化;并通過si RNA沉默抑制Kv1.3表達,使用MTT和流式細胞儀觀察對h PDLFs的增殖和分化成骨能力的作用。結(jié)果細胞免疫熒光結(jié)果顯示與健康組比較,牙周炎組h PDLFs中Kv1.3顯著低表達(P0.05);Western blot結(jié)果顯示Kv1.3-si RNA能夠明顯抑制細胞周期蛋白D1(Cyclin D1)和C-myc蛋白表達(P0.05);流式細胞儀檢測顯示Kv1.3-si RNA能夠顯著抑制細胞G2/M期;同時,Western blot結(jié)果顯示Kv1.3-si RNA明顯降低堿性磷脂酶(ALP)和骨鈣素(OCN)蛋白表達。結(jié)論抑制Kv1.3表達能夠明顯抑制h PDLFs的增殖和分化成骨能力。
[Abstract]:Objective to investigate the role of Kv1.3 in the proliferation and differentiation of human periodontal ligament fibroblasts (PDLFs). Methods periodontal ligament fibroblasts were isolated and cultured from healthy subjects and patients with periodontitis in vitro. The expression of Kv1.3 in PDLFs of periodontitis was detected by Q-PCR and Western blot, and the expression of Kv1.3 was inhibited by si RNA silencing. The effects of MTT and flow cytometry on the proliferation and differentiation of h PDLFs were observed. Results the results of cellular immunofluorescence showed that compared with the healthy group, In the periodontitis group, the expression of Kv1.3 in PDLFs was significantly lower than that in the control group. The results of Western blot showed that Kv1.3-si RNA could significantly inhibit the expression of cyclin D1(Cyclin D1) and C-myc protein, and the flow cytometry showed that Kv1.3-si RNA could significantly inhibit the G 2 / M phase of the cells. At the same time, the results of Western blot showed that Kv1.3-si RNA significantly decreased the expression of alkaline phospholipase (ALP) and osteocalcin (OCN) protein. Conclusion inhibition of Kv1.3 expression can significantly inhibit the proliferation and differentiation of h PDLFs.
【作者單位】： 中國人民解放軍第105醫(yī)院口腔科;中國人民解放軍第105醫(yī)院檢驗科;安徽醫(yī)科大學口腔醫(yī)學院安徽醫(yī)科大學附屬口腔醫(yī)院安徽省口腔疾病研究中心實驗室;安徽醫(yī)科大學第四附屬醫(yī)院口腔科;
【基金】：安徽省科技計劃項目(編號:12070403062)
【分類號】：R781.4

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