發(fā)布時間：2018-05-01 08:11

  本文選題：牙菌斑生物膜 + 牙釉質(zhì)��； 參考：《河北大學(xué)》2016年碩士論文

【摘要】：齲病作為一種多發(fā)性的細菌性疾病,如不能得到及時的治療會導(dǎo)致繼發(fā)性根尖周炎、牙髓炎,甚至牙冠的完全損壞,嚴重影響人類的身心健康和生活質(zhì)量。如何實現(xiàn)齲病的高效、精準(zhǔn)的早期診斷并制定相應(yīng)的防治方案,是目前牙科醫(yī)學(xué)領(lǐng)域一大熱點問題。近年來,納米生物材料因其獨特的尺寸效應(yīng)和易于修飾的特點已被廣泛應(yīng)用于牙科醫(yī)學(xué)研究中,尤其是在齲病的預(yù)防和治療方面表現(xiàn)出潛在的應(yīng)用和轉(zhuǎn)化前景。本研究利用納米合成技術(shù),開發(fā)了一種基于兩親性材料TR4的納米藥物,并將其成功應(yīng)用于齲齒的早期預(yù)防和可視化評價。該納米藥物在齲齒的防治方面具有如下特點和優(yōu)勢:(1)顯著的齲齒預(yù)防作用;(2)可視化的預(yù)防和評價能力;(3)穩(wěn)定的納米結(jié)構(gòu)。本研究主要包括以下研究內(nèi)容:1、聚集誘導(dǎo)發(fā)光性質(zhì)的TR4分子的合成及表征。應(yīng)用Fmoc固相多肽合成法合成TR4多肽部分,并將棕櫚酸(PA)偶聯(lián)到多肽上,將羧基化的四苯乙烯偶聯(lián)到賴氨酸的氨基上得到TR4分子。結(jié)果表明,合成的TR4分子質(zhì)量為1395.73,分子濃度超過一定值后可以自組裝成納米粒子,并且在聚集狀態(tài)下誘導(dǎo)TPE發(fā)出熒光。2、聚集誘導(dǎo)發(fā)光性質(zhì)的TR4分子對齲病防治作用的研究。以牙菌斑生物膜和牙釉質(zhì)為研究模型,針對TR4分子對齲病的預(yù)防效果進行研究。研究結(jié)果表明,TR4分子可以和牙菌斑生物膜結(jié)合形成納米粒子,并被產(chǎn)氨菌屬利用產(chǎn)氨,中和產(chǎn)酸菌屬代謝產(chǎn)生的乳酸,將牙菌斑生物膜環(huán)境維持在脫礦值(pH 5.5)以上。同時TR4分子可以和牙釉質(zhì)結(jié)合,抑制牙釉質(zhì)的脫礦。通過共聚焦顯微鏡技術(shù)觀察TR4同牙菌斑生物膜孵育不同時間后的熒光強度變化,發(fā)現(xiàn)牙菌斑生物膜中的產(chǎn)氨菌屬可以利用TR4中的精氨酸。3、聚集誘導(dǎo)發(fā)光性質(zhì)的TR4分子對齲病評價作用的研究。將TR4分子和牙菌斑生物膜共孵育后,TR4分子吸附在牙菌斑生物膜和牙釉質(zhì)表面形成納米聚集態(tài),并發(fā)出熒光。通過熒光分光光度計技術(shù)驗證了TR4熒光強度同牙菌斑生物膜的量有著良好的線性關(guān)系,并且當(dāng)牙釉質(zhì)脫礦后,脫礦部位熒光強度變?nèi)跎踔料�。因�?通過熒光強度的變化可以實現(xiàn)判斷牙菌斑生物膜的覆蓋程度及牙齒脫礦情況,從而達到評價齲病發(fā)生的幾率和治療效果的目的。
[Abstract]:As a multiple bacterial disease, caries can not be treated in a timely manner, which can lead to secondary periapical periodontitis, pulpitis, and even the complete crown of the teeth, which seriously affect the physical and mental health and quality of life of human beings. How to achieve high efficiency of dental caries, accurate early diagnosis and the formulation of corresponding prevention and control schemes are the leading dentistry In recent years, nanoscale biomaterials have been widely used in dental research because of their unique size effects and easy modification, especially in the prevention and treatment of dental caries. This study has developed a kind of two parent material, TR4, by using nanotechnology. The nanoscale drug has been successfully applied to the early prevention and visual evaluation of dental caries. The nanometers have the following characteristics and advantages in the prevention and control of dental caries: (1) significant dental caries prevention; (2) visual prevention and evaluation ability; (3) stable nanostructures. This study mainly includes the following research content: 1, aggregation induced hair The synthesis and characterization of the TR4 molecules of light properties. The synthesis of TR4 polypeptide by Fmoc solid phase polypeptide synthesis, and the coupling of palmitic acid (PA) to the polypeptide, the carboxylation of four styrene coupling to the amino group of lysine, the TR4 molecule is obtained. The results show that the synthesized TR4 molecular mass is 1395.73, and the molecular concentration exceeds a certain value and can be self assembled. A study of the effect of the TR4 molecule on the prevention and control of caries by inducing TPE to emit fluorescence.2 in the aggregation state and to induce the luminescent properties of TR4 molecules. The study model of dental plaque biofilm and dental enamel is used to study the preventive effect of TR4 molecules on caries. The results show that TR4 molecules can be formed in combination with dental plaque biofilm. Nanoparticles, which are used in ammonia production, and lactic acid producing acid producing lactic acid, maintain the plaque biofilm environment above the demineralization value (pH 5.5). At the same time, the TR4 molecules can combine with the enamel to inhibit the demineralization of the enamel. The fluorescence of the TR4 and the plaque biofilm at different time is observed by confocal microscopy. It is found that the genus aspartate in the biofilm of dental plaque can use the arginine.3 in TR4 to study the evaluation of caries by the aggregation induced TR4 molecules of luminescent properties. After incubating the TR4 molecules and dental plaque biofilms, TR4 molecules adsorb on the plaque biofilm and the enamel surface to form nanoscale aggregation states and emit fluorescence. The fluorescence spectrophotometer proved that the fluorescence intensity of TR4 has a good linear relationship with the amount of dental plaque biofilm, and the fluorescence intensity of the demineralized part becomes weaker or even disappeared when the enamel is demineralized. Therefore, the coverage of the plaque and the demineralization of the dental plaque can be judged by the change of fluorescence intensity, thus the evaluation of the tooth demineralization can be achieved. The probability of the occurrence of the caries and the purpose of the therapeutic effect.

【學(xué)位授予單位】：河北大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R781.1
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本文編號：1828464