本文選題：牙骨質(zhì) + 牙周組織��； 參考：《山東醫(yī)藥》2017年26期

【摘要】：目的觀察保留健康牙骨質(zhì)對人牙周韌帶細(xì)胞分化的影響。方法取正畸患者拔除的健康前磨牙,從牙根的近中和遠(yuǎn)中面釉牙骨質(zhì)界下2 mm制備對稱的5 mm×4 mm的牙片43對,隨機分成觀察組(保留牙骨質(zhì))和對照組(去除牙骨質(zhì))各43張。刮取牙周膜組織,按組織塊貼壁法培養(yǎng)牙周韌帶細(xì)胞,分離培養(yǎng)后,取兩組各23張牙片,將細(xì)胞接種到牙片上共培養(yǎng),制成PDLC-牙片復(fù)合體。7 d后刮除并收集PDLC-牙片復(fù)合體上的細(xì)胞,采用RT-PCR方法檢測成牙骨質(zhì)標(biāo)記物牙骨質(zhì)附著蛋白(CAP)和牙骨質(zhì)蛋白23(CP-23)表達(dá)。取兩組剩余的20張牙片制成細(xì)胞-牙片復(fù)合體放入半透明膜中,植入裸鼠體內(nèi),8周后比較兩組牙片表面新形成的硬組織情況,并采用免疫組化方法觀察骨橋蛋白(OPN)和骨唾液蛋白(BSP)表達(dá)。結(jié)果觀察組CAP和CP-23表達(dá)較對照組升高(P均0.01)。植入裸鼠體內(nèi),8周后觀察組14張牙片顯示原有牙骨質(zhì)上有牙骨質(zhì)樣基質(zhì)形成;新舊牙骨質(zhì)間并未觀察到裂隙。對照組17張牙片在牙根表面有纖維組織形成,并且新纖維組織和牙本質(zhì)表面間均可觀察到不同寬度的裂隙。新形成的牙骨質(zhì)樣基質(zhì)中,骨橋蛋白和骨唾液蛋白均呈陽性。結(jié)論保留健康牙根表面的牙骨質(zhì)可能促進人牙周韌帶細(xì)胞向成牙骨質(zhì)細(xì)胞分化,有利于牙周組織愈合。
[Abstract]:Objective to observe the effect of preserving healthy cementum on the differentiation of human periodontal ligament cells. Methods healthy premolars extracted from orthodontic patients were randomly divided into two groups: the control group and the control group, and 43 pairs of symmetrical 5 mm 脳 4 mm dental slices were prepared from the proximal and distal enamel bone boundary 2 mm below the bone boundary of the tooth root, and were randomly divided into two groups: the observation group (reserved cementum) and the control group (removing cementum). Periodontal ligament cells were cultured according to the tissue block adherence method. After isolation and culture, 23 pieces of teeth were taken from each group, and the cells were co-cultured on the dental slices. The cells of PDLC- dentin complex were scraped and collected after 7. 7 d. The expression of cemental attachment protein (CAP) and cemental protein 23 (CP-23) were detected by RT-PCR. The cell-dental complex was made from the remaining 20 teeth of the two groups and put into the translucent membrane. After 8 weeks of implantation in nude mice, the newly formed hard tissues on the surface of the teeth were compared between the two groups. The expression of osteopontin (OPN) and bone saliva protein (BSPs) were observed by immunohistochemistry. Results the expression of CAP and CP-23 in the observation group was higher than that in the control group (P < 0.01). After implanted into nude mice for 8 weeks, 14 dental slices in the observation group showed cement-like matrix formation on the original cementum, but no fissure was observed between the new and old cementum. In the control group, there was fibrous tissue formation on the root surface of 17 teeth, and cracks of different widths could be observed between the new fibrous tissue and the dentin surface. Osteopontin and bone saliva were positive in newly formed cementoid matrix. Conclusion the preservation of cementum on the surface of healthy root may promote the differentiation of human periodontal ligament cells into cementoblasts, which is beneficial to the healing of periodontal tissue.
【作者單位】： 濟南市口腔醫(yī)院;
【基金】：山東省優(yōu)秀中青年科學(xué)家科研獎勵基金(BS2013YY056)
【分類號】：R781.4

【相似文獻(xiàn)】

相關(guān)期刊論文 前5條

1 苗蘭卿;人牙骨質(zhì)表層微細(xì)構(gòu)造變化的電鏡觀察——關(guān)于夏皮氏纖維的變化[J];國外醫(yī)學(xué).口腔醫(yī)學(xué)分冊;1982年03期

2 王曉儀;;根折后牙骨質(zhì)生長修復(fù)[J];國外醫(yī)學(xué)參考資料.口腔醫(yī)學(xué)分冊;1974年02期

3 張淑琴;趙小芳;;牙骨質(zhì)及其組織工程的研究進展[J];甘肅醫(yī)藥;2011年02期

4 陳明,劉宏偉;兔骨髓基質(zhì)細(xì)胞與自體牙骨質(zhì)片的體外附著生長[J];第四軍醫(yī)大學(xué)學(xué)報;2004年05期

5 ;[J];;年期

相關(guān)博士學(xué)位論文 前2條

1 高永波;人類牙骨質(zhì)的納米結(jié)構(gòu)表征[D];吉林大學(xué);2012年

2 孫銘;EMD、PTH對人PDL細(xì)胞成骨/成牙骨質(zhì)表型調(diào)控機制的研究[D];吉林大學(xué);2005年

相關(guān)碩士學(xué)位論文 前4條

1 周麗淑;miR-10a-5p調(diào)控人牙周膜干細(xì)胞向成骨/成牙骨質(zhì)方向分化的研究[D];福建醫(yī)科大學(xué);2015年

2 任肖華;骨髓基質(zhì)細(xì)胞與牙骨質(zhì)生成關(guān)系的體外實驗研究[D];中國人民解放軍軍醫(yī)進修學(xué)院;2002年

3 陳明;兔骨髓基質(zhì)細(xì)胞附著于自體牙骨質(zhì)片生長的體外研究[D];第一軍醫(yī)大學(xué);2004年

4 呂影濤;牙根損傷修復(fù)動物模型的建立及其修復(fù)機制初探[D];南方醫(yī)科大學(xué);2011年

，

本文編號：1814407