發(fā)布時(shí)間：2018-04-24 06:31

  本文選題：肥胖 + 牙周炎　； 參考：《南方醫(yī)科大學(xué)》2017年碩士論文

【摘要】：動(dòng)物研究發(fā)現(xiàn),B-1a細(xì)胞能在感染性炎癥或慢性炎癥疾病的固有免疫機(jī)制中起到保護(hù)作用,它能在病毒或細(xì)菌入侵的早期快速清除感染源。牙周炎與肥胖,可分別引起局部感染性炎癥與全身慢性炎癥狀態(tài),二者相關(guān)聯(lián)的流行病學(xué)報(bào)道很多,但是它們之間的免疫機(jī)制還尚不明了。本研究建立肥胖復(fù)合牙周炎小鼠模型,基于B-1a細(xì)胞存在牙周病損局部和肥胖與牙周炎相關(guān)聯(lián)的理論基礎(chǔ),對(duì)B-1a細(xì)胞在肥胖和牙周炎兩種炎癥狀態(tài)中發(fā)揮的病理機(jī)制進(jìn)行探索。第一章肥胖狀態(tài)下牙周感染對(duì)小鼠牙周組織中B-1a細(xì)胞的影響1.目的本章主要探討肥胖狀態(tài)下牙周感染對(duì)小鼠牙周組織中B-1a細(xì)胞的表達(dá)水平及功能的影響,分析肥胖伴牙周感染小鼠牙周組織中B-1a細(xì)胞膜表面特異性蛋白CD5,及B-1a細(xì)胞分泌的白細(xì)胞介素-10(IL-10)炎癥因子和抗Ⅰ膠原抗體(抗COLⅠ抗體)的蛋白和mRNA表達(dá)變化。2.方法動(dòng)物模型分組:將24只小鼠隨機(jī)分成兩組,每組各12只。其中一組喂以高脂飼料,為肥胖組(high fat diet,HF),另一組喂以低脂飼料,為正常體重組(low fat diet,LF)。飼料誘導(dǎo)30周后,分別從肥胖組和正常體重組中隨機(jī)抽取6只做牙周真性結(jié)扎處理(periodontitis,P),每組剩余的6只做假性結(jié)扎處理(control,C),結(jié)扎處理5天,隨即產(chǎn)生牙周炎組(LFP組)、正常體重對(duì)照組(LFC組)、肥胖組(HFC組)、肥胖復(fù)合牙周炎組(HFP組)。免疫組化染色定量分析和實(shí)時(shí)定量PCR基因水平檢測(cè):通過實(shí)時(shí)定量PCR和免疫組化染色方法對(duì)牙周組織中CD5、抗COL Ⅰ抗體、IL-10的蛋白和mRNA表達(dá)水平進(jìn)行定量檢測(cè)。牙周組織中CD5、抗COL Ⅰ抗體、IL-10的表達(dá)水平分別與體重和牙周組織浸潤的炎癥細(xì)胞數(shù)之間的相關(guān)性分析:小鼠稱重;牙周組織HE染色后對(duì)浸潤的炎癥細(xì)胞定量計(jì)數(shù);將體重、炎癥細(xì)胞數(shù)與三種蛋白之間進(jìn)行Pearson相關(guān)性分析。3.結(jié)果牙周組織中B-1a細(xì)胞的mRNA及蛋白的表達(dá)水平:B-1a細(xì)胞相關(guān)三種蛋白的牙周炎主效應(yīng)均有顯著性,牙周炎組中的IL-10、CD5、抗COLⅠ抗體的mRNA和蛋白表達(dá)水平均顯著高于對(duì)照組(P0.001)。肥胖和牙周炎對(duì)牙周組織中B-1a細(xì)胞功能的影響:CD5、IL-10、抗COLⅠ抗體的蛋白表達(dá)水平與炎癥細(xì)胞數(shù)之間均呈顯著正相關(guān)性(r0,P0.001);但三者與體重之間的相關(guān)性均不顯著(P0.05)。4.結(jié)論B-1a細(xì)胞在牙周炎癥早期表達(dá)上調(diào),所分泌的抗COL Ⅰ抗體可以抵御細(xì)菌初期對(duì)牙周組織的破壞。但肥胖并未對(duì)炎性牙周組織中B-1a細(xì)胞的表達(dá)水平及功能產(chǎn)生顯著性影響。第二章肥胖狀態(tài)下牙周感染對(duì)小鼠脾臟組織中B-1a細(xì)胞的影響1.目的本章主要探討肥胖狀態(tài)下牙周感染對(duì)小鼠脾臟組織中B-1a細(xì)胞表達(dá)水平及功能的影響,分析肥胖伴牙周感染小鼠脾臟組織中B-1a細(xì)胞膜表面特異性蛋白CD5,及B-1a細(xì)胞分泌的抗COL Ⅰ抗體和IL-10炎癥因子的蛋白表達(dá)變化。2.方法蛋白質(zhì)印跡定量分析:用蛋白質(zhì)印跡方法對(duì)脾臟組織中CD5、IL-10、抗COL Ⅰ抗體的蛋白表達(dá)水平進(jìn)行定量檢測(cè)。將脾臟組織中CD5、IL-10、抗COL Ⅰ抗體的表達(dá)水平分別與體重和牙周組織浸潤的炎癥細(xì)胞數(shù)之間進(jìn)行Pearson相關(guān)性分析。3.結(jié)果脾臟組織中B-1a細(xì)胞的表達(dá)水平:B-1a細(xì)胞相關(guān)三種蛋白的肥胖主效應(yīng)均有顯著性,肥胖組中的CD5、IL-10、抗COLⅠ抗體的蛋白表達(dá)水平均顯著高于正常體重組(P0.001)。肥胖和牙周炎對(duì)脾臟組織中B-1a細(xì)胞功能的影響:CD5、IL-10、抗COLⅠ抗體的蛋白表達(dá)水平與體重之間均呈顯著正相關(guān)性(r0,P0.001),但三者與牙齦炎癥細(xì)胞數(shù)之間的相關(guān)性均不顯著(P0.05)。4.結(jié)論B-1a細(xì)胞在肥胖機(jī)體內(nèi)表達(dá)上調(diào),但其發(fā)揮的功能尚不明確。牙周感染并未對(duì)肥胖機(jī)體脾臟組織中B-1a細(xì)胞的表達(dá)產(chǎn)生顯著性影響。
[Abstract]:Animal studies have found that B-1a cells can play a protective role in the inherent immune mechanism of infectious inflammation or chronic inflammatory diseases. It can quickly clear the source of infection in the early stages of virus or bacterial invasion. Periodontitis and obesity can cause local infection and systemic chronic inflammatory disease. The two related epidemiological reports are very important. Many, but the immune mechanism between them is still unknown. This study established a model of obese and complex periodontitis in mice. Based on the theoretical basis of the association between periodontal lesions and obesity and periodontitis, B-1a cells were explored in the two inflammatory states of B-1a cells in obesity and periodontitis. The first chapter was the obesity state. The effect of periodontal infection on B-1a cells in the periodontal tissue of mice 1. the purpose of this chapter is to investigate the effect of periodontal infection on the expression level and function of B-1a cells in the periodontal tissue of the mice, and to analyze the specific protein CD5 of the B-1a cell membrane in the periodontal tissues of the obese and periodontal infected mice, and the interleukocyte secreted by B-1a cells. -10 (IL-10) inflammatory factor and anti collagen antibody (anti COL I antibody) protein and mRNA expression change.2. method animal model group: 24 mice were randomly divided into two groups, 12 rats in each group. One group was fed with high fat diet, the obese group (high fat diet, HF), the other group fed with low fat diet, for the normal weight group (low fat diet, low). After 30 weeks of induction, 6 patients were randomly selected from the obese group and the normal weight group for periodontitis (P). The remaining 6 of each group were treated with pseudoligature (control, C) and ligation for 5 days. Then the periodontitis group (group LFP), the normal weight control group (group LFC), the obese group (group HFC), and the obesity complex periodontitis group (group HFP). Quantitative analysis of immunohistochemical staining and real-time quantitative PCR gene level detection: quantitative detection of CD5, anti COL I antibody, IL-10 protein and mRNA expression level in periodontal tissues by real-time quantitative PCR and immunohistochemical staining. The expression of CD5, anti COL I antibody, and IL-10 expression level in periodontal tissues with body weight and periodontal tissue infiltration respectively Correlation analysis between the number of inflammatory cells: weighing in mice; quantitative counting of infiltrating inflammatory cells after HE staining in periodontal tissue; Pearson correlation analysis between body weight, number of inflammatory cells and three kinds of proteins; the mRNA and protein of B-1a cells in periodontal tissues were reached: the periodontitis owner of three kinds of protein related proteins in the periodontal tissue. The effect of IL-10, CD5, anti COL I antibody in the periodontitis group was significantly higher than that of the control group (P0.001). The effect of obesity and periodontitis on the function of B-1a cells in periodontal tissue: the protein expression level of CD5, IL-10, anti COL I antibody and the number of inflammatory cells was significantly positive correlation (R0, P0.001). The correlation between the three and the weight was not significant (P0.05).4. conclusion the expression of B-1a cells was up-regulated in the early periodontitis, and the secreted anti COL I antibody could resist the early destruction of periodontal tissue. But obesity did not have a significant effect on the expression level and function of B-1a cells in the inflammatory periodontitis. The second chapter of obesity was in the state of obesity. The effect of periodontal infection on the B-1a cells in the spleen tissue of mice 1. the purpose of this chapter is to investigate the effect of periodontal infection on the expression and function of B-1a cells in the spleen tissues of the mice, and to analyze the specific protein CD5 of the B-1a cell membrane in the spleen tissues of the obese and periodontal infected mice, and the anti COL I anti B-1a cells secreted by B-1a cells. Protein expression changes in body and IL-10 inflammatory factors.2. method of Western blot quantitative analysis: the protein expression level of CD5, IL-10, anti COL I antibody in spleen tissues was quantified by Western blot. The expression of CD5, IL-10, anti COL I antibody in the spleen tissues was divided with body weight and infiltration of periodontitis. Pearson correlation analysis between the numbers of.3. results in the expression level of B-1a cells in the spleen tissue: the main effect of obesity on three proteins related to B-1a cells was significant. The protein expression level of CD5, IL-10, anti COL I antibody in the obese group was significantly higher than that of the normal weight group (P0.001). The B-1a cell work in the spleen tissues of obesity and periodontitis Effect: the protein expression level of CD5, IL-10, anti COL I antibody showed significant positive correlation with body weight (R0, P0.001), but the correlation between the three and the number of gingivitis cells was not significant (P0.05).4. conclusion B-1a cells were up regulated in the body of obesity, but the function of the cells was not clear. Periodontal infection was not on the obese body. The expression of B-1a cells in spleen tissue has a significant effect.

【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781.4

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