本文選題：人牙釉質(zhì) + 酸蝕�。� 參考：《西南交通大學(xué)》2014年博士論文

【摘要】：人體天然牙是人體內(nèi)最重要的咀嚼器官,在人的一生中行使重要功能。牙冠最外層的牙釉質(zhì)是人體內(nèi)最硬的組織,對(duì)咀嚼磨耗具有較好的抵抗力。人體口腔環(huán)境十分復(fù)雜,除常見的因咀嚼需要產(chǎn)生的機(jī)械行為外,牙齒最外層的牙釉質(zhì)還經(jīng)常遭受內(nèi)源性或外源性酸性介質(zhì)引起的酸蝕,進(jìn)而誘發(fā)牙齒過度磨損。隨著酸性飲料攝入量的增大和人類平均壽命的提高,牙齒酸蝕問題日趨嚴(yán)重,酸蝕已經(jīng)成為現(xiàn)代社會(huì)人類牙齒過度磨損的主要誘因。因此,開展人牙釉質(zhì)酸蝕損傷及再礦化修復(fù)行為的系統(tǒng)研究具有非常重要的理論意義和現(xiàn)實(shí)意義。本文主要采用納米壓痕/劃痕儀,試驗(yàn)研究了體外再礦化對(duì)酸蝕人牙釉質(zhì)納米力學(xué)性能和微摩擦學(xué)行為的影響。使用掃描電子顯微鏡(SEM)、電子能譜儀(EDX)、表面輪廓儀、激光共聚焦掃描顯微鏡(LCSM)、X射線衍射分析(XRD)等材料表面分析測試設(shè)備,對(duì)牙釉質(zhì)的再礦化表面進(jìn)行了詳細(xì)的觀察和分析。所取得的主要結(jié)論如下：(1)在0.001 M檸檬酸介質(zhì)(pH=3.25)中,當(dāng)人牙釉質(zhì)的酸蝕時(shí)問不大于10 min時(shí),牙釉質(zhì)次表層由于酸蝕脫礦形成的軟化層厚度為微納米尺度,約為500～1000 nm。隨著酸蝕時(shí)間增加,酸蝕的軟化層厚度呈現(xiàn)非線性增大。軟化層的厚度與牙釉質(zhì)表面酸蝕損傷程度密切相關(guān),牙釉質(zhì)表面酸蝕損傷越嚴(yán)重,軟化層厚度越大。(2)在人工唾液介質(zhì)中經(jīng)過12h體外再礦化處理后,在酸蝕人牙釉質(zhì)試樣表面生成一層與原始人牙釉質(zhì)成分相似、但生長方向不同的礦化結(jié)晶,這層晶體在C軸無明顯生長取向。體外再礦化處理后,酸蝕人牙釉質(zhì)表面的納米力學(xué)性能和微觀摩擦學(xué)性能均有顯著提高,再礦化表面的納米壓痕硬度和彈性模量顯著高于酸蝕表面,磨損量明顯低于酸蝕表面。但是,再礦化表面的納米力學(xué)性能和微摩擦學(xué)性能仍明顯低于人牙釉質(zhì)原始表面,這說明在給定的實(shí)驗(yàn)條件下,人牙釉質(zhì)表面的酸蝕損傷可以通過再礦化得到一定程度的修復(fù),但無法完全修復(fù)。(3)CPP-ACP具有修復(fù)牙釉質(zhì)酸蝕損傷的能力。在2.0% w/v CPP-ACP溶液中經(jīng)12 h體外再礦化處理后,酸蝕人牙釉質(zhì)表面形成一層不均勻的無定型態(tài)礦物沉積,表面礦物質(zhì)含量、硬度及彈性模量均增大,表面摩擦力、摩擦系數(shù)和磨損量均下降。但是,再礦化表面的納米力學(xué)性能和耐磨性仍明顯低于牙釉質(zhì)原始表面。由于無定型態(tài)的再礦化沉積物和健康牙釉質(zhì)的HAP晶體之間存在明顯的結(jié)構(gòu)差異,因此,盡管CPP-ACP溶液中鈣、磷酸根離子濃度高于人工唾液,但是它對(duì)酸蝕人牙釉質(zhì)表面納米力學(xué)性能和微摩擦學(xué)行為的修復(fù)依然不徹底。(4)由于化學(xué)組成、微觀結(jié)構(gòu)和晶體特性的差異,乳牙釉質(zhì)的酸蝕耐受性較恒牙釉質(zhì)差,在相同的酸蝕條件下,乳牙釉質(zhì)的酸蝕損傷重于恒牙釉質(zhì)。在2.0% w/vCPP-ACP溶液中經(jīng)過12h的體外再礦化處理后,乳牙釉質(zhì)表面由于酸蝕導(dǎo)致的形貌損傷、納米力學(xué)性能下降和微摩擦學(xué)行為改變均得到了一定程度的修復(fù)。鑒于乳牙釉質(zhì)的酸蝕耐受性較恒牙釉質(zhì)差,相同酸蝕時(shí)間乳牙釉質(zhì)表面脫礦較恒牙釉質(zhì)嚴(yán)重,因此,乳牙釉質(zhì)的再礦化修復(fù)作用弱于恒牙釉質(zhì)。(5)酸蝕時(shí)間、再礦化工況和生命活性物質(zhì)均會(huì)影響牙釉質(zhì)表面酸蝕損傷的再礦化修復(fù)。酸蝕時(shí)間越短,牙釉質(zhì)的再礦化表面越平整,再礦化沉積物的分布越均勻致密。攪拌不利于酸蝕牙釉質(zhì)的再礦化修復(fù),和靜止工況相比,攪拌工況下酸蝕牙釉質(zhì)再礦化表面的再礦化沉積物排列疏松,分布不均勻,脫礦導(dǎo)致的蜂窩結(jié)構(gòu)仍然隱約可見。延長酸蝕人牙釉質(zhì)在人工唾液介質(zhì)中的再礦化處理時(shí)間,有助于再礦化表面生成排列有序的片狀HAP晶體。人體天然全唾液中的蛋白等有機(jī)物對(duì)酸蝕人牙釉質(zhì)的再礦化修復(fù)存在誘導(dǎo)和促進(jìn)作用,和人工唾液介質(zhì)相比,酸蝕牙釉質(zhì)在人體天然全唾液介質(zhì)中再礦化處理12h后,再礦化表面生成的晶體更均勻,排列更整齊,表面耐磨性較好。
[Abstract]:The human natural tooth is the most important masticatory organ in the human body and plays an important role in the human life. The enamel of the outer layer of the crown is the most hard tissue in the human body and has a good resistance to the chewing wear. The human oral environment is very complex, and the outer enamel of the teeth is also the most common in addition to the mechanical behavior required by chewing. Acid erosion caused by endogenous or exogenous acid medium is often caused by acid corrosion, which induces excessive wear of teeth. With the increase of acid drink intake and the increase of human life expectancy, the problem of dental erosion is becoming more and more serious. Acid erosion has become the main cause of excessive wear of human teeth in modern society. Therefore, the acid erosion damage of human tooth enamel is carried out. The systematic study of remineralization and remineralization is of great theoretical and practical significance. In this paper, the effects of remineralization on the mechanical properties and Microtribological behavior of acid etching human tooth enamel were studied by nano indentation / scratch tester. The scanning electron microscopy (SEM), EDX, surface profilometer were used to study the effect of remineralization in vitro. Laser confocal scanning microscope (LCSM), X ray diffraction analysis (XRD) and other materials surface analysis and testing equipment were used to observe and analyze the enamel remineralized surface in detail. The main conclusions are as follows: (1) the enamel subsurface in the 0.001 M citric acid medium (pH=3.25) when the acid etching of the human tooth enamel is not more than 10 min The thickness of the softening layer formed by the acid erosion demineralization is micro nano scale, which is about 500~1000 nm. with the increase of etching time, the thickness of the softening layer of the acid corrosion increases nonlinear. The thickness of the softening layer is closely related to the degree of acid erosion damage on the enamel surface, the more serious the erosion of the enamel surface, the greater the thickness of the softening layer. (2) artificial saliva. After remineralization of 12h in vitro, a layer of mineralized crystallization similar to the original human tooth enamel was formed on the surface of the enamel on the surface of the acid etching human tooth enamel, with no obvious growth orientation on the C axis. After remineralization, the nanomechanical and Microtribological properties of the acid etching human enamel surface were both. The nano indentation hardness and modulus of elasticity of remineralized surface are significantly higher than that of the acid corrosion surface, and the wear amount is obviously lower than that of the etching surface. However, the nanomechanical properties and Microtribological properties of the remineralized surface are still lower than the original surface of the human tooth enamel, which shows the acid erosion of the enamel surface under the given experimental conditions. The injury can be remineralized to a certain extent, but it can not be repaired completely. (3) CPP-ACP has the ability to repair dental enamel corrosion damage. After remineralizing in 2% w/v CPP-ACP solution by 12 h in vitro, the acid etching enamel surface forms an inhomogeneous amorphous mineral deposit, surface mineral content, hardness and elasticity The modulus increased, the surface friction, the friction coefficient and the wear amount were all decreased. However, the nanomechanical properties and wear resistance of the remineralized surface were still significantly lower than the original surface of the enamel. There was a significant structural difference between the remineralized sediments and the HAP crystals of the healthy enamel. Therefore, the calcium and phosphorus in the CPP-ACP solution The concentration of acid root ion is higher than that of artificial saliva, but it is still not thorough to repair the nanomechanical properties and Microtribological behavior of the enamel surface. (4) the acid corrosion resistance of the deciduous enamel is worse than that of the permanent tooth enamel because of the chemical composition, microstructure and crystal characteristics, and the acid erosion of the enamel of the deciduous teeth under the same etching condition The injury was heavier than the enamel of permanent teeth. After 12h remineralization in 2% w/vCPP-ACP solution, the surface of the enamel surface was damaged by acid erosion, the decline of nanomechanical properties and the change of Microtribological behavior were repaired to a certain extent. The demineralization of enamel surface is more serious than that of the permanent tooth enamel. Therefore, the remineralization repair effect of the enamel of the deciduous teeth is weaker than that of the permanent enamel. (5) the time of etching, the remineralization condition and the life active substance will affect the remineralization of the enamel surface erosion damage. The shorter the time of the etching, the more smooth the remineralized surface of the enamel is, the distribution of the remineralized sediment The more uniform and compact, the agitation is not conducive to the remineralization of the enamel of acid etching. Compared with the static condition, the remineralized sediments on the enamel remineralized surface under the stirring condition are loosely arranged and the distribution is uneven, and the honeycomb structure caused by demineralization is still invisible. Inter mineralized surface contributes to the formation of ordered lamellar HAP crystals. The protein and other organic compounds in human natural whole saliva induce and promote the remineralization of the acid etching human enamel. Compared with the artificial saliva medium, the acid etching enamel remineralized 12h in the human natural whole saliva and remineralized the surface. The crystals are more uniform, arranged more orderly and have better surface wear resistance.

【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2014
【分類號(hào)】：R783

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉曉靜,鄭寶山,王濱濱,王明仕;茶防齲研究進(jìn)展[J];地球與環(huán)境;2005年02期

2 楊敏,鄭昌瓊,冉均國,鐘毓茂,張欣苑;鈦基-TiN-TiC系梯度薄膜材料的生物摩擦磨損特性研究[J];功能材料;2000年02期

3 劉敏,張加理;牙釉質(zhì)和羥基磷灰石晶體的體外合成[J];國外醫(yī)學(xué)(醫(yī)學(xué)地理分冊(cè));1998年02期

4 高晉華,杜民權(quán),臺(tái)保軍;牙齒酸蝕癥[J];國外醫(yī)學(xué).口腔醫(yī)學(xué)分冊(cè);2005年05期

5 楚金普;周學(xué)東;;牙齒硬組織脫礦與再礦化的研究方法[J];國外醫(yī)學(xué).口腔醫(yī)學(xué)分冊(cè);2006年01期

6 李文卿;趙瑋;;控制牙齒酸蝕癥的研究進(jìn)展[J];國際口腔醫(yī)學(xué)雜志;2008年04期

7 李利;周學(xué)東;;齲病相關(guān)細(xì)菌的細(xì)菌素[J];國際口腔醫(yī)學(xué)雜志;2009年01期

8 陳舟,羅宗蓮,楊松;含氟牙膏對(duì)釉質(zhì)脫礦影響的體外研究[J];華西口腔醫(yī)學(xué)雜志;2001年01期

9 施文元;周學(xué)東;;人體口腔微生物群的相互作用[J];華西口腔醫(yī)學(xué)雜志;2010年01期

10 薛晶;肖麗英;周學(xué)東;;人體口腔微生物組研究最新進(jìn)展[J];華西口腔醫(yī)學(xué)雜志;2010年01期

相關(guān)博士學(xué)位論文 前2條

1 鄭靖;牙齒的摩擦學(xué)特性研究[D];西南交通大學(xué);2004年

2 葛俊;牙釉質(zhì)和骨的分級(jí)結(jié)構(gòu)和納米力學(xué)性能研究[D];清華大學(xué);2005年

相關(guān)碩士學(xué)位論文 前4條

1 黃毅;人牙釉質(zhì)的微結(jié)構(gòu)納米力學(xué)性能及微摩擦磨損行為研究[D];西南交通大學(xué);2009年

2 肖楓;人牙釉質(zhì)的酸蝕特性研究[D];西南交通大學(xué);2009年

3 黃歡;人體天然牙在檸檬酸介質(zhì)中的腐蝕磨損特性研究[D];西南交通大學(xué);2010年

4 石茂渝;氟/激光處理對(duì)人牙釉質(zhì)酸蝕和耐磨性能的影響[D];西南交通大學(xué);2013年

，

本文編號(hào)：1794311