本文選題：轉染時機　切入點：電穿孔　出處：《重慶醫(yī)學》2017年20期

【摘要】：目的通過觀察不同時機轉染基因?qū)ο骂M骨牽引區(qū)細胞周期蛋白表達的影響,探討基因治療促進牽引成骨的分子機制及最佳時機。方法 48只新西蘭大白兔建立雙側下頜骨牽引成骨模型后均分為A、B、C、D組。A、B、C組分別于術后即刻、術后第4天、術后第14天在牽引區(qū)轉染重組質(zhì)粒pIRES-hBMP2-hVEGF165,并局部給予電穿孔刺激。4組動物均在術后第4天,以1mm/d的速度持續(xù)牽引10d后進入固定期。于固定期第7、14、28、56天各組分別處死動物3只,通過免疫組織化學染色檢查牽引區(qū)新生骨組織cyclin A、D1、E的表達。結果在固定期第7、14天時,A、B、C組牽引間隙cyclin A、D1、E的表達較強,在第7天時表達最強烈,并且A、B、C組均較D組表達強;第28、56天時各組表達均較弱。在第7天時,B組表達較A、C、D組強(P0.05),A、C組間表達差異無統(tǒng)計學意義(P0.05),但均較D組強(P0.05);在第14天時,B、C組表達較A、D組強(P0.05),B、C組間及A、D組間表達差異無統(tǒng)計學意義(P0.05)。結論 cyclin A、D1、E的高表達能促進牽引區(qū)細胞分裂、增殖和分化,進而加快牽引區(qū)新骨形成,牽引期是基因治療干預下頜骨牽引成骨的最佳時機。
[Abstract]:Objective to investigate the molecular mechanism and optimal time of gene therapy for promoting distraction osteogenesis by observing the effect of gene transfection on the expression of cyclin in mandibular traction region.Methods Forty-eight New Zealand white rabbits were divided into two groups after bilateral mandibular distraction osteogenesis.The recombinant plasmid pIRES-hBMP2-hVEGF165was transfected into the traction region on the 14th day after operation, and the animals in the group of local electroporation were treated with electroporation stimulation on the 4th day after operation. The animals in the control group were treated with 1mm/d at the rate of 1mm/d for 10 days and then entered the fixed period.Three animals were killed in each group on day 7, 14, 28 and 56 of the fixed period, and the expression of cyclin Agna D _ (1) E was detected by immunohistochemical staining in the nascent bone tissue of the traction area.Results the expression of cyclin in the tractive space was stronger in group A than in group C on day 7 and day 7, and the expression in group C was stronger than that in group D on day 7, and the expression was weaker in group C on day 2856.Conclusion the high expression of cyclin Agna D1OE can promote the cell division, proliferation and differentiation in the traction area, and then accelerate the formation of new bone in the traction area. The distraction stage is the best time for gene therapy to interfere with mandibular distraction osteogenesis.
【作者單位】： 西南醫(yī)科大學附屬醫(yī)院外科;濟寧醫(yī)學院附屬醫(yī)院燒傷整形科;南京醫(yī)科大學附屬友誼整形外科醫(yī)院;
【基金】：國家自然科學基金資助項目(30600653) 四川省衛(wèi)生廳科研課題(12226)
【分類號】：R782

【相似文獻】

相關期刊論文 前6條

1 周峻,楊連甲,董紹忠,李媛;細胞周期蛋白A在舌癌中的表達及意義[J];浙江腫瘤;1999年04期

2 周峻,楊連甲,董紹忠,李媛;舌癌組織及細胞系中細胞周期蛋白D基因的轉錄與蛋白的合成[J];實用口腔醫(yī)學雜志;2000年01期

3 周峻,楊連甲,金巖,董紹忠,李媛;細胞周期蛋白A對舌鱗癌細胞周期和抗原含量的影響[J];細胞與分子免疫學雜志;2001年02期

4 周峻,楊連甲,何勇,董紹忠,李媛;細胞周期蛋白A正反義基因?qū)θ松喟┘毎鲋车挠绊慬J];中華口腔醫(yī)學雜志;2001年02期

5 劉正;;一項具有重要價值的研究[J];中華口腔醫(yī)學雜志;2006年11期

6 周曉健;余楊;潘紅芽;陳萬濤;張萍;張志愿;;細胞周期蛋白D1反義寡核苷酸調(diào)控聯(lián)合順鉑抑制口腔鱗癌耐藥細胞的研究[J];中華口腔醫(yī)學雜志;2006年06期

相關博士學位論文 前1條

1 周峻;細胞周期相關蛋白在舌癌中的表達及正反義cyclin A基因轉染對舌癌細胞Tca8113的生物學效應[D];第四軍醫(yī)大學;2000年

，

本文編號：1717634