本文選題：舌鱗癌　切入點：瘤內(nèi)淋巴管　出處：《天津醫(yī)科大學(xué)》2014年碩士論文

【摘要】：研究目的：1、以淋巴管特異性標(biāo)記物癌胚抗原相關(guān)細(xì)胞粘附因子1(CEACAM1)標(biāo)記人舌鱗癌組織中淋巴管,并計算淋巴管密度(LMVD)值,觀察CEACAM1在人舌鱗癌瘤內(nèi)及瘤周淋巴管中的表達(dá)情況,探討CEACAM1及其受體在舌癌發(fā)生、發(fā)展和淋巴轉(zhuǎn)移中的作用及意義。 2、將舌鱗癌細(xì)胞株正位植入裸鼠左側(cè)舌緣構(gòu)建舌鱗癌動物模型,從而模擬舌體內(nèi)環(huán)境,觀察舌癌細(xì)胞的生物學(xué)行為及舌鱗癌模型的淋巴結(jié)轉(zhuǎn)移情況,采用免疫組織化學(xué)染色的方法標(biāo)記舌鱗癌病灶及轉(zhuǎn)移淋巴結(jié)中的淋巴管,并觀察腫瘤淋巴管的情況及其與舌鱗癌淋巴道轉(zhuǎn)移的相關(guān)性。 研究方法：1、收集72例舌鱗癌病灶及轉(zhuǎn)移淋巴結(jié)的病理標(biāo)本,其中43例(男性24例,女性19例)人舌鱗癌頸淋巴結(jié)轉(zhuǎn)移陽性患者的組織和29例(男性17例,女性12例)人舌鱗癌頸淋巴結(jié)轉(zhuǎn)移陰性患者的組織,采用免疫組織化學(xué)染色方法檢測病理組織中的表達(dá),用兔抗人CEACAM1標(biāo)記淋巴管,并計數(shù)人舌鱗癌瘤內(nèi)與瘤周組織淋巴管生成的密度,并進(jìn)行統(tǒng)計學(xué)分析。2、將人舌鱗癌Tca83細(xì)胞懸液正位移植于裸鼠左側(cè)舌緣,隔日觀察并記錄裸鼠體重、舌體成瘤情況、病灶的大小,待裸鼠自然死亡或體質(zhì)量下降至原體質(zhì)量的25%時,切取舌部病灶及頸部淋巴結(jié),進(jìn)行HE染色和免疫組化檢測舌鱗癌組織的CEACAM1表達(dá),用兔抗人CEACAM1標(biāo)記淋巴管,并計數(shù)人舌鱗癌瘤內(nèi)與瘤周組織淋巴管生成的密度,并進(jìn)行統(tǒng)計學(xué)分析。 研究結(jié)果：1、人舌鱗癌病理標(biāo)本的癌灶內(nèi)可見散在的、孤立的新生不完整管狀結(jié)構(gòu),未與周圍宿主淋巴管相連通,這些管狀結(jié)構(gòu)內(nèi)可見較多的內(nèi)皮細(xì)胞間隙,管腔細(xì)窄；瘤周可見淋巴管數(shù)量增多、管腔擴(kuò)張、開放。通過淋巴管計數(shù),瘤周淋巴管密度(16.748±3.070)高于瘤內(nèi)淋巴管密度(8.662±3.214),差異有顯著性(P0.01)。 2、裸鼠在接種后第6-12天相繼出現(xiàn)舌部腫瘤,成瘤率為77.14%,通過HE染色及免疫組化染色檢測的淋巴結(jié)轉(zhuǎn)移陽性率為34.29%,鏡下可見舌鱗癌瘤內(nèi)和瘤周內(nèi)都有陽性表達(dá),表現(xiàn)為淋巴管形態(tài)不規(guī)則,管壁薄,可呈開放狀態(tài),腔內(nèi)不含紅細(xì)胞,與血管可明顯區(qū)別,有時有膠凍狀淋巴液；也可表現(xiàn)為管腔塌陷、閉鎖呈條索狀,甚至可見單個內(nèi)皮細(xì)胞圍成管腔。通過淋巴管計數(shù),瘤周淋巴管密度(5.488-2.114)高于瘤內(nèi)淋巴管密度(3.026±1.470),差異具有顯著性(P0.01)。 研究結(jié)論：1、(1) CEACAMl對淋巴管內(nèi)皮細(xì)胞具有很強的特異性與敏感性,可廣泛用于淋巴管的研究。(2)舌鱗癌的瘤內(nèi)和瘤周組織中,特別是瘤周組織存在淋巴管生成,增加了腫瘤細(xì)胞淋巴轉(zhuǎn)移的機會,在舌鱗癌的生長和轉(zhuǎn)移過程中發(fā)揮了重要作用。 2、成功建立裸鼠舌鱗癌正位模型,為研究在體內(nèi)環(huán)境中舌癌的生長、轉(zhuǎn)移機制提供了重要的實驗手段。通過比較裸鼠舌鱗癌瘤內(nèi)淋巴管密度與瘤周淋巴管密度,推斷淋巴管的生成可能對舌鱗癌淋巴道轉(zhuǎn)移有一定促進(jìn)作用。
[Abstract]:Objective to investigate the expression of CEACAM1 in human tongue squamous cell carcinoma (LMVD), and to investigate the expression of CEACAM1 in human tongue squamous cell carcinoma (LMVD), using lymphangio-specific marker carcinoembryonic antigen associated cell adhesion factor 1 CEACAM1, and to calculate the density of lymphatic vessels in human tongue squamous cell carcinoma (LMVD), and to observe the expression of CEACAM1 in human tongue squamous cell carcinoma and its surrounding lymphatic vessels. To investigate the role and significance of CEACAM1 and its receptors in the development, development and lymphatic metastasis of tongue cancer. 2. The tongue squamous cell carcinoma cell line was implanted into the left tongue margin of nude mice to construct the animal model of tongue squamous cell carcinoma, and the biological behavior of tongue cancer cell line and lymph node metastasis of tongue squamous cell carcinoma model were observed. The lymphatic vessels in squamous cell carcinoma of tongue and metastatic lymph nodes were labeled by immunohistochemical staining, and the correlation between lymphatic vessels and lymphatic metastasis in squamous cell carcinoma of tongue was observed. Methods 72 specimens of squamous cell carcinoma of tongue and metastatic lymph nodes were collected, including 43 cases (24 males, 19 females) and 29 cases (17 males) of cervical lymph node metastasis of tongue squamous cell carcinoma. The expression of lymphatic vessels was detected by immunohistochemical staining in 12 cases of human tongue squamous cell carcinoma with negative cervical lymph node metastasis. Rabbit anti-human CEACAM1 was used to label lymphatic vessels. The density of lymphangiogenesis in and around human tongue squamous cell carcinoma was counted, and the density of lymphangiogenesis was analyzed statistically. The Tca83 cell suspension of human tongue squamous cell carcinoma was transplanted into the left lingual margin of nude mice. The body weight and the tumorigenesis of tongue were observed and recorded every other day. When the size of the lesion was reduced to 25% of the mycoplasma mass in nude mice, the tongue lesion and cervical lymph nodes were removed, and the expression of CEACAM1 in tongue squamous cell carcinoma was detected by HE staining and immunohistochemistry. The lymphatic vessels were labeled with rabbit anti-human CEACAM1. The density of lymphatic vessels in and around human tongue squamous cell carcinoma was counted and analyzed statistically. Results: 1. In the pathological specimens of human tongue squamous cell carcinoma, scattered and isolated new incomplete tubular structures were found, which were not connected with the lymphatic vessels of the surrounding host. There were more endothelial cell interspaces and narrow lumen in these tubular structures. The number of lymphatic vessels increased, the lumen dilated and opened around the tumor, and the density of lymphatic vessels around the tumor was 16.748 鹵3.070), which was higher than that of the intratumoral lymphatic vessels (8.662 鹵3.214), and the difference was significant (P 0.01). 2. Tongue tumors appeared in nude mice 6-12 days after inoculation, the tumorigenesis rate was 77.14 and the positive rate of lymph node metastasis detected by HE staining and immunohistochemical staining was 34.29. The lymphatic vessels are characterized by irregular morphology, thin wall, open state, no red blood cells in the lumen, which can be distinguished from the blood vessels, sometimes with gelatinous lymphatic fluid; they can also be shown as cavities collapsing, atresia being striped, The density of lymphatic vessels around the tumor (5.488-2.114) was higher than that of the intratumoral lymphatic vessels (3.026 鹵1.470), and the difference was significant (P 0.01). Conclusion CEACAMl has strong specificity and sensitivity to lymphatic endothelial cells, and can be widely used in the study of lymphatic vessels. 2) lymphangiogenesis exists in intratumor and perineoplastic tissues of tongue squamous cell carcinoma, especially in surrounding tissues. It increases the chance of lymphatic metastasis of tumor cells and plays an important role in the growth and metastasis of tongue squamous cell carcinoma. 2. The positive position model of tongue squamous cell carcinoma in nude mice was successfully established, which provided an important experimental means for studying the growth and metastasis mechanism of tongue carcinoma in vivo. It is concluded that lymphangiogenesis may promote lymphatic metastasis in squamous cell carcinoma of tongue.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.86

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