本文選題：成釉細胞瘤　切入點：侵襲性　出處：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:檢測腫瘤相關(guān)巨噬細胞(tumor-associated macrophages,TAMs)和基質(zhì)金屬蛋白酶-2(matrix metalloproteinases-2,MMP-2)在成釉細胞瘤中的表達情況,探討二者相關(guān)性及與成釉細胞瘤中微血管密度(microvessel density,MVD)的關(guān)系,初步探討二者在成釉細胞瘤侵襲性生長中的作用機制。方法:收集成釉細胞瘤病理石蠟組織塊43例作為研究對象,另收集正常口腔黏膜組織10例作為對照,采用免疫組織化學(xué)(Immunohistochemistry,IHC)通用二步法分別檢測兩組中CD68標(biāo)記的巨噬細胞、MMP-2和CD34標(biāo)記的微血管密度的表達情況,結(jié)果判定后統(tǒng)計學(xué)處理分析,進一步討論。結(jié)果:1、TAMs在ABs中的計數(shù)為27.48±17.64,主要表達于腫瘤基質(zhì);MMP-2在所有ABs中均有不同程度表達,主要表達于腫瘤實質(zhì);MVD在ABs中的計數(shù)為21.84±8.20。2、TAMs、MMP-2和MVD在ABs中的表達均高于正常黏膜組織,差異有統(tǒng)計學(xué)意義(P0.01)。3、在ABs中,MMP-2表達和TAMs計數(shù)呈正相關(guān)關(guān)系(rs=0.350,P0.05),MVD計數(shù)隨著TAMs和MMP-2的表達強度增加而增加,均成正相關(guān)關(guān)系(r=0.454,P0.01;rs=0.459,P0.01)。結(jié)論:1、TAMs和MMP-2在ABs中均有顯著表達,參與ABs的發(fā)生發(fā)展;2、TAMs和MMP-2在ABs中的表達具有相關(guān)性,可能發(fā)揮協(xié)同作用;3、ABs中MVD較正常黏膜組織高,微血管生成參與腫瘤進展,TAMs和MMP-2可能發(fā)揮誘導(dǎo)血管生成作用促進ABs的侵襲性生長。
[Abstract]:Objective: to investigate the expression of tumor-associated macrophage tumor-associated macrophages (TAMs) and matrix metalloproteinases-2m-2 (MMP-2) in ameloblastoma, and to explore the relationship between them and microvessel density (MVD) in ameloblastoma.To explore the mechanism of the two in the invasive growth of ameloblastoma.Methods: 43 cases of ameloblastoma and 10 cases of normal oral mucosa were collected.The expression of MMP-2 and CD34 labeled microvessel density in macrophages labeled with CD68 in two groups were detected by Immunohistochemistry-IHC general two-step method. The results were analyzed by statistical analysis and discussed further.Results the count of Tams in ABs was 27.48 鹵17.64, which was mainly expressed in the tumor matrix, MMP-2, in all ABs. The expression of MMP-2 and MVD in ABs were 21.84 鹵8.20.2t in ABs. The expression of MMP-2 and MVD in ABs was higher than that in normal mucosa.There was a positive correlation between the expression of MMP-2 and the count of TAMs in ABs. The number of MVD increased with the increase of TAMs and MMP-2 expression, and the positive correlation was found between the expression of MMP-2 and the count of TAMs.Conclusion both Tams and MMP-2 were significantly expressed in ABs. The expression of Tams and MMP-2 in ABs was correlated with the occurrence and development of ABs, and the MVD expression in Tams was higher than that in normal mucosa.Microangiogenesis involved in tumor progression Tams and MMP-2 may play a role in angiogenesis and promote the invasive growth of ABs.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.8

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