本文選題：季銨鹽　切入點(diǎn)：銀離子　出處：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：[目的]評(píng)估季銨鹽包裹溴化銀納米復(fù)合物(AgBr/NPVP)對(duì)聚甲基丙烯酸甲酯基樹脂(Poly-methyl methacrylate(PMMA)-based dental resins,下文簡(jiǎn)稱:PMMA樹脂)抗菌性能、細(xì)胞毒性及機(jī)械性能的影響。[方法]通過原位沉淀法合成AgBr/NPVP,采用傅立葉紅外光譜(FTIR)及1H核磁共振(1HNMR)對(duì)新合成的NPVP進(jìn)行表征,X線衍射(XRD)及透射電子顯微鏡(TEM)對(duì)新合成的AgBr/NPVP進(jìn)行表征,利用激光粒度分析儀測(cè)定AgBr/NPVP的粒徑大小;運(yùn)用液體稀釋法測(cè)定AgBr/NPVP對(duì)白色念珠菌(C.albicans)ATCC90028的最小抑菌濃度(MIC)、最小殺菌濃度(MFC);場(chǎng)發(fā)射掃描電鏡(Fe-SEM)觀察 C.albicans ATCC90028 與 1× MFC AgBr/NPVP 混懸液接觸24 h后的細(xì)胞形態(tài)變化;制備含不同質(zhì)量分?jǐn)?shù)AgBr/NPVP的PMMA樹脂試件,運(yùn)用直接接觸法、死/活菌染色法評(píng)估改性PMMA樹脂的抗菌性能,通過WST-8試驗(yàn)及FTIR分別對(duì)改性PMMA樹脂細(xì)胞毒性及轉(zhuǎn)化率(DC)進(jìn)行檢測(cè);樹脂撓曲強(qiáng)度(FS)、撓曲模量(FM)及維氏顯微硬度分別采用萬能試驗(yàn)機(jī)及維氏硬度計(jì)進(jìn)行檢測(cè)。[結(jié)果]通過原位沉淀法成功合成了 AgBr/NPVP,其粒徑大小為(30.86±1.46)nm。AgBr/NPVP 對(duì) C.albicans ATCC90028 的 MIC 及 MFC 值均為250 μg/ml。Fe-SEM 示 C.albicans ATCC90028 與 1× MFC AgBr/NPVP 混懸液接觸24 h后,正常細(xì)胞形態(tài)喪失,細(xì)胞完整性破壞,胞體皺縮、內(nèi)陷,部分細(xì)胞裂解成碎片。PMMA樹脂隨AgBr/NPVP添加量的增加,抗菌活性不斷增強(qiáng),老化2周后,各組抗菌率均呈穩(wěn)定趨勢(shì),0.3%組抗菌率達(dá)80%以上。激光共聚焦顯微鏡(CLSM)觀察顯示,改性PMMA樹脂表面粘附及存活的細(xì)菌均較空白組少。根據(jù)ISO10993-5標(biāo)準(zhǔn),改性PMMA樹脂對(duì)人牙髓細(xì)胞(HDPCs)毒性較低。改性實(shí)驗(yàn)各組PMMA樹脂聚合24 h后,DC均達(dá)70%以上。FS及FM值實(shí)驗(yàn)各組與對(duì)照組間無統(tǒng)計(jì)學(xué)差異(p0.05),0.1%及0.2%組維氏硬度值與對(duì)照組間無顯著差異(p0.05),但當(dāng)AgBr/NPVP添加量為0.3%時(shí),其維氏顯微硬度值有所下降,且差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。[結(jié)論]經(jīng)適量AgBr/NPVP改性的聚甲基丙烯酸甲酯基樹脂對(duì)白色念珠菌ATCC90028具有一定抗菌作用,且對(duì)其細(xì)胞毒性及理化性能無顯著影響。
[Abstract]:[objective] to evaluate the effects of quaternary ammonium salt encapsulated silver bromide nanocomposite (AgBr-NPVP) on the antibacterial properties, cytotoxicity and mechanical properties of Poly-methyl methacrylate(PMMA)-based dental resinses.[methods] AgBr-r / NPVP was synthesized by in situ precipitation method. The newly synthesized NPVP was characterized by FTIR and 1H NMR). The newly synthesized AgBr/NPVP was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM).The particle size of AgBr/NPVP was measured by laser particle size analyzer.The minimal inhibitory concentration and minimal bactericidal concentration of AgBr/NPVP against C. albicansa ATCC90028 were determined by liquid dilution method, and the morphological changes of C.albicans ATCC90028 and 1 脳 MFC AgBr/NPVP suspension were observed 24 h after contact with C.albicans ATCC90028 and 1 脳 MFC AgBr/NPVP suspension.PMMA resin samples with different mass fraction of AgBr/NPVP were prepared. The antibacterial properties of modified PMMA resin were evaluated by direct contact method and death / live bacteria staining method. The cytotoxicity and conversion rate of modified PMMA resin were detected by WST-8 test and FTIR respectively.The flexural strength, modulus of flexure and Vickers microhardness of resin were tested by universal testing machine and Vickers hardness tester respectively.[results] AgBr-P / NPVP was successfully synthesized by in situ precipitation method. The particle size of AgBr-P / NPVP was 30.86 鹵1.46)nm.AgBr/NPVP and the MIC and MFC values of C.albicans ATCC90028 were 250 渭 g/ml.Fe-SEM respectively. The results showed that the normal cell morphology was lost and the cell integrity was destroyed after C.albicans ATCC90028 was exposed to 1 脳 MFC AgBr/NPVP suspension for 24 h.With the increase of AgBr/NPVP content, the antibacterial activity of some cells crumpled and trapped. After 2 weeks of aging, the antimicrobial rate of each group showed a stable trend of 80% or more.The observation of laser confocal microscope showed that the surface adhesion and survival of the modified PMMA resin were less than that of the blank group.According to ISO10993-5 standard, the modified PMMA resin is less toxic to human pulp cells.The Vickers microhardness decreased and the difference was statistically significant (P 0.05).[conclusion] AgBr/NPVP modified polymethyl methacrylate resin has a certain antibacterial effect on Candida albicans ATCC90028, and has no significant effect on its cytotoxicity and physical and chemical properties.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R783.1

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