本文選題：環(huán)孢素A　切入點：親環(huán)素A　出處：《第二軍醫(yī)大學(xué)》2014年碩士論文

【摘要】：研究目的 本實驗通過構(gòu)建CIGO動物模型，通過免疫組織化學(xué)和Western blot的方法檢測增生牙齦中CyPA、EMMPRIN（CD147）、MMP-2、MMP-9的表達(dá)，旨在探討CIGO的發(fā)病機(jī)制。 研究方法 1.選取48只SD雄性大鼠，按隨機(jī)數(shù)字表法分為CsA實驗組和對照組，依據(jù)每日測量實際體重實驗組給予CsA注射液腹部皮下注射，對照組給予適量蓖麻油皮下注射；從開始給藥起，每周觀察大鼠牙齦增生情況。 2.連續(xù)給藥4周后，處死全部動物，運(yùn)用影像學(xué)檢查大鼠牙槽骨變化情況。 3.分離磨牙牙齦，運(yùn)用組織學(xué)的方法觀察牙齦的變化。 4.運(yùn)用免疫組化的方法，檢測CyPA、CD147、MMP-2、MMP-9在CsA實驗組和對照組中的表達(dá)和定位。 5.運(yùn)用Western blot的方法，檢測CyPA、CD147、MMP-2、MMP-9四種蛋白在CsA實驗組和對照組的表達(dá)量。 結(jié)果 1.大體觀察結(jié)果：CsA實驗組大鼠在連續(xù)注射CsA2周后，牙齦組織開始出現(xiàn)增生；經(jīng)過CsA注射液的持續(xù)注射，4周后大鼠牙齦增生明顯，牙齦外形如球狀、結(jié)節(jié)狀，隆起于牙齒表面，相鄰牙齦乳頭向牙冠方向增生明顯，齦溝加深，，形成假性牙周袋，牙齦組織失去正常形態(tài)，牙周探診未見出血，無牙周附著喪失；對照組牙齦未見增生。建模成功。 2. Micro CT檢查結(jié)果：實驗組與對照組大鼠均無牙槽骨喪失，牙槽骨高度無變化。 3. Western blot結(jié)果：CsA實驗組中CyPA、MMP-2、MMP-9蛋白表達(dá)與對照組相比明顯降低，但CD147蛋白表達(dá)在兩組間無明顯差異。 4.免疫組化結(jié)果：與對照組相比，CsA實驗組中CyPA、MMP-2和MMP-9免疫組化陽性表達(dá)顯著降低，差異具有統(tǒng)計學(xué)意義(P0.01)；而CD147的陽性表達(dá)在兩組間無明顯變化，差異不具有統(tǒng)計學(xué)意義(P0.05)。 結(jié)論 CsA可能通過下調(diào)CyPA的表達(dá)，影響了CyPA與CD147的相互作用，使CyPA-CD147復(fù)合物介導(dǎo)的MMPs信號通路受到影響，下調(diào)了MMPs的表達(dá)，抑制了膠原的降解，引起膠原生成的相對增多，形成藥物性牙齦增生。
[Abstract]:Research purpose. In this study, CIGO animal model was established, and the expression of MMP-9 in proliferative gingiva was detected by immunohistochemistry and Western blot. The aim of this study was to explore the pathogenesis of CIGO. Research method. 1. Forty-eight male SD rats were randomly divided into two groups: CsA group and control group. The experimental group was subcutaneously injected with CsA injection and the control group was subcutaneously injected with castor oil according to daily weight measurement. Gingival hyperplasia was observed weekly in rats. 2. After 4 weeks of continuous administration, all the animals were killed and the alveolar bone was examined by imaging. 3. The gingiva of molars was separated and the changes of gingiva were observed by histological method. 4. Immunohistochemical method was used to detect the expression and localization of MMP-9 in CsA group and control group. 5. Western blot was used to detect the expression of MMP-9 in CsA group and control group. Results. 1. The results of gross observation showed that gingival tissue began to proliferate after continuous injection of CsA2 for weeks in CSA group, and gingival hyperplasia appeared in rats after 4 weeks of continuous injection of CsA injection, the gingival shape was spherical and nodular, and it was protruding on the surface of teeth. The adjacent gingival papilla accreases to the crown direction obviously, the gingival groove deepens, forms the false periodontal bag, the gingival tissue loses the normal form, the periodontal diagnosis does not see the bleeding, has no periodontal attachment loss; the control group does not see the gingival hyperplasia. 2. Results of Micro CT: there was no alveolar bone loss and no change in alveolar bone height in the experimental group and the control group. 3. The Western blot results showed that the expression of CyPA-MMP-2MMP-9 in the CSA group was significantly lower than that in the control group, but there was no significant difference in the expression of CD147 protein between the two groups. 4. Immunohistochemical results: compared with the control group, the positive expression of CyPAA MMP-2 and MMP-9 in CSA group was significantly decreased, the difference was statistically significant (P 0.01), but the positive expression of CD147 had no significant change between the two groups, but the difference was not statistically significant (P 0.05). Conclusion. CsA may affect the interaction between CyPA and CD147 by down-regulating the expression of CyPA, affect the MMPs signaling pathway mediated by CyPA-CD147 complex, down-regulate the expression of MMPs, inhibit the degradation of collagen, and cause the relative increase of collagen production. The formation of drug-induced gingival hyperplasia.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R781.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李吉;謝紅付;易梅;彭樂芳;雷丹;陳翔;簡丹;;親環(huán)素A和CD147在皮膚衰老過程中表達(dá)的研究(英文)[J];中南大學(xué)學(xué)報(醫(yī)學(xué)版);2011年03期

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